Addgene

Showing: 6241 - 6260 of 10571 results

Results per page:

Sort results by:

PX458-3xHA-en15FnCas9

Plasmid

#201948

Purpose

Mammalian expression plasmid of enhanced activity FnCas9 variant en15FnCas9 with T2A-EGFP and cloning backbone for sgRNA

Depositor

Debojyoti Chakraborty

Article

Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

Insert

3xHA-NLS-en15FnCas9-T2A-EGFP

Use

CRISPR

Tags

3xHA, NLS, and T2A-EGFP

Expression

Mammalian

Mutation

E1603H on FnCas9

Promoter

Cbh

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-ESR1-Y537S

Plasmid

#116374

Purpose

Lentiviral expression of ESR1 Y537S

Depositor

Kenneth Scott, Gordon Mills

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ESR1 (ESR1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Y537S

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pPtGE35

Plasmid

#107999

Purpose

Expresses TevCas9 in Phaeodactylum tricornutum / Encodes elements required for conjugation

Depositor

David Edgell

Article

Slattery et al ACS Synth Biol. 2018 Jan 8. doi: 10.1021

Inserts

OriT
40SRPS8 Promoter
ShBle
40SRPS8 Terminator
Cen6-ArsH4-His3
I-TevI nuclease and partial linker domain

Use

CRISPR and Synthetic Biology; Episomal vector for…

Tags

Cas9

Expression

Mutation

Promoter

Available since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q48

Plasmid

#111747

Purpose

Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

Depositor

Cheryl Arrowsmith

Article

Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

Insert

HTT (HTT Human)

Use

Baculovirus expression

Tags

FLAG

Expression

Mammalian

Mutation

Q48 (polyQ repeat)

Promoter

CMV and P10

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-mFgf21pro3-luc

Plasmid

#101799

Purpose

Reporter for mouse Fgf21 promoter activity

Depositor

Seiichi Oyadomari

Article

Miyake et al FASEB J. 2016 Feb;30(2):798-812. doi: 10

Insert

Fibroblast growth factor 21 promoter (Fgf21 Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q73

Plasmid

#111728

Purpose

Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells

Depositor

Cheryl Arrowsmith

Article

Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70

Insert

HTT (HTT Human)

Use

Baculovirus expression

Tags

FLAG

Expression

Mammalian

Mutation

Q73 (polyQ repeat)

Promoter

CMV and P10

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-EGFR-G719A

Plasmid

#116253

Purpose

Lentiviral expression of EGFR G719A

Depositor

Kenneth Scott, Gordon Mills

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EGFR (EGFR Human)

Use

Lentiviral

Tags

Expression

Mutation

G719A

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpp1-is3

Plasmid

#58247

Purpose

Expression vector producing isoform 3 derived mouse osteopontin (EGFP tagged)

Depositor

Georgios Stathopoulos

Article

Giannou et al J Clin Invest. 2015 Jun;125(6):2317-34.

Insert

Spp1 isoform 3 (Spp1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 30, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-PDGFRA-Y288C

Plasmid

#116453

Purpose

Lentiviral expression of PDGFRA Y288C

Depositor

Kenneth Scott, Gordon Mills

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

PDGFRA (PDGFRA Human)

Use

Lentiviral

Tags

Expression

Mutation

Y288C

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-SMO

Plasmid

#116792

Purpose

Lentiviral expression of SMO

Depositor

Kenneth Scott, Gordon Mills

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

SMO (SMO Human)

Use

Lentiviral

Tags

Expression

Mutation

WT

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
NGFR P210

Plasmid

#27486

Depositor

Warren Pear

Article

He et al Blood. 2002 Apr 15;99(8):2957-68.

Insert

bcr/abl (P210) (ABL1, BCR Human)

Use

Retroviral

Tags

IRES and NFGR

Expression

Mammalian

Mutation

Contains the "complete" bcr/abl fusion

Promoter

Available since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-AKT1

Plasmid

#116710

Purpose

Lentiviral expression of AKT1

Depositor

Kenneth Scott, Gordon Mills

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

AKT1 (AKT1 Human)

Use

Lentiviral

Tags

Expression

Mutation

WT

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-QKI-6 [N182/17R-2b]

Plasmid

#206661

Purpose

Mammalian Expression Plasmid of anti-QKI-6 (Human). Derived from hybridoma N182/17-2b.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-QKI-6 (Homo sapiens) recombinant Mouse monoclonal antibody (QKI Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-NFE2L2

Plasmid

#116765

Purpose

Lentiviral expression of NFE2L2

Depositor

Kenneth Scott, Gordon Mills

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

NFE2L2 (NFE2L2 Human)

Use

Lentiviral

Tags

Expression

Mutation

WT

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-TrpC6 [N490/24R]

Plasmid

#211590

Purpose

Mammalian Expression Plasmid of anti-TrpC6 (Human) IgG2a R-mAb. Derived from hybridoma N490/24.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-TrpC6 (Homo sapiens) recombinant mouse monoclonal antibody. (TRPC6 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-EGFR-A763_Y764insFQEA

Plasmid

#116233

Purpose

Lentiviral expression of EGFR A763_Y764insFQEA

Depositor

Kenneth Scott, Gordon Mills

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EGFR (EGFR Human)

Use

Lentiviral

Tags

Expression

Mutation

A763_Y764insFQEA

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CAG-FRT-Rev-3xGFP

Plasmid

#191204

Purpose

FlpO dependent GFP expression

Depositor

Janelia Viral Tools

Article

Winnubst et al Cell. 2019 Sep 19;179(1):268-281.e13. do

Insert

3X GFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-Bassoon [L124/59R]

Plasmid

#177470

Purpose

Mammalian Expression Plasmid of anti-Bassoon (Rat). Derived from hybridoma L124/59.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Bassoon (Rattus norvegicus) recombinant mouse monoclonal antibody (Bsn Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
TRIPZ-bioGEF-SUMO1nc-PURO

Plasmid

#208042

Purpose

Enables inducible expression of low-affinity Avitag-SUMO1nc; to perform bioE3; nc = non-cleavable mutation; selection with puromycin

Depositor

Rosa Barrio, James Sutherland

Article

Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:

Insert

SUMO1nc (SUMO1 Human)

Use

Lentiviral

Tags

Low-affinity Avitag

Expression

Mutation

Q94P Mutation near C-terminus suppresses cleavage…

Promoter

tetO/UBC

Available since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Anti-Beta4-spectrin [N393/2R]

Plasmid

#177541

Purpose

Mammalian Expression Plasmid of anti-Beta4-spectrin (Human). Derived from hybridoma N393/2.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Beta4-spectrin (Homo sapiens) recombinant mouse monoclonal antibody (SPTBN4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge