We narrowed to 21,063 results for: ACE
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Plasmid#188511PurposeExpresses FLAG tagged SpRY dCas9 VPRDepositorInsertdCas9
UseCRISPR and LentiviralExpressionMammalianMutationD10A/A61R/H840A/L1111R/D1135L/S1136W/G1218K/E1219…PromoterEF1aAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEasyG3_nat
Plasmid#184918PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_nat recombines in vivo with a PCR product from pEasyG3_mic.DepositorInsertgRNA scaffold
UseCRISPRExpressionBacterialAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEasyG3_mic
Plasmid#184920PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_mic recombines in vivo with a PCR product from pEasyG3_zeo/nat/hph.DepositorInsertgRNA scaffold
UseCRISPRExpressionBacterialAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST exorh:mGreenLantern
Plasmid#195988PurposeTransgenesis backboneDepositorTypeEmpty backboneUseOtherAvailable SinceFeb. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CBA-DIO-GCaMP6s-P2A-mBeRFP-WPRE-pA
Plasmid#175177PurposeCre-dependent GCaMP6s and mBeRFP expressionDepositorInsertGCaMP6s
UseAAVTagsP2A-mBeRFPPromoterCAGAvailable SinceDec. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC-fFuCas9-HTBNLS-hph
Plasmid#121092PurposeMultigene editing in the Fusarium fujikuroi genome using the CRISPR-Cas9 systemDepositorInsertCas9 and hygromycin resist gene
UseCRISPRExpressionBacterialPromotergpdA;trpCAvailable SinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
amilCP chromoprotein promoter-less
Plasmid#118008PurposeFor course in "Synthetic Biology: A Lab Manual"DepositorInsertRBS, amilCP
UseSynthetic Biology; Escherichia coliAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pre_IV_CS10_1545_v1
Plasmid#126861Purpose4x 1545 b long scaffolds divided by Zn-dependent self-cleaving DNAzyme cassettes, from compatibility group 10, made by method IV, yields linear 1033 b long scaffolds after Zn incubationDepositorInsertIV_CS10_1545_v1
UseSynthetic BiologyAvailable SinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK1_008
Plasmid#123671PurposeEncodes ConLS and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK systemDepositorInsertConnector LS-2xHS4
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK1_009
Plasmid#123672PurposeEncodes ConL1 and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK systemDepositorInsertConnector L1-2xHS4
ExpressionMammalianAvailable SinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
Carl-CeNL(Ca2+)_110μ-KDEL-pcDNA3
Plasmid#111925PurposeCyan color luminescent Ca2+ indicator targeted to endoplasmic reticulum. Calreticulin and a KDEL signal for ER retention located at the N-terminus and C-terminus of CeNL(Ca2+)_110μDepositorInsertCeNL(Ca2+)_110μ
TagsCalreticulin and KDELExpressionMammalianMutationE31D, F92W, E104D. D133E at CaMPromoterCMVAvailable SinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSin_EF1a_tagBFP_P2A_NLS_pMag_CreC60_IRES_PuroR
Plasmid#176311PurposeExpresses NLS-pMag-CreC60 with tagBFP markerDepositorInserttagBFP_P2A_NLS_pMag_CreC60_IRES_PuroR
UseLentiviral and Synthetic BiologyTagstagBFPExpressionMammalianPromoterhuman EF1alphaAvailable SinceFeb. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEX-SaCas9-U6-sgGrin2b
Plasmid#124851PurposeMutagenesis of Grin2bDepositorInsertGrin2b (Grin2b Mouse)
UseAAV, CRISPR, and Mouse TargetingAvailable SinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEXfrt-SaCas9-U6-sgGrin2b
Plasmid#124864PurposeMutagenesis of Grin2bDepositorInsertGrin2b (Grin2b Mouse)
UseAAV, CRISPR, and Mouse TargetingAvailable SinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
HiBiT-FZD1
Plasmid#195839PurposeExpresses human FZD1 with an N-terminal HiBiTDepositorAvailable SinceMarch 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
MTK1_013
Plasmid#123676PurposeEncodes ConL5 and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK systemDepositorInsertConnector L5-2xHS4
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
cyt-GafD-mTurboID-V5
Plasmid#184640PurposeExpresses V5-tagged GlycoID construct: GafD_short-miniTurboID in mammalian cytosol for O-GlcNAc interactome taggingDepositorInsertcyt-GlycoID-V5
TagsNuclear export sequence and V5 tagExpressionMammalianMutationGafD -> expressed aa22-178 ; BirA* -> expre…PromoterCMVAvailable SinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
WT C-His Ana GvpC in pET28a
Plasmid#85732PurposeExpresses His-tagged GvpC from Anabaena flos-aquae in E.coliDepositorInsertGas vesicle protein C (his-tagged)
TagsHis-tagExpressionBacterialMutationAdded an extra glycine after the methionine at th…PromoterT7Available SinceMarch 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pre_IV_CS10_1033_v1
Plasmid#126860Purpose4x 1033 b long scaffolds divided by Zn-dependent self-cleaving DNAzyme cassettes, from compatibility group 10, made by method IV, yields linear 1033 b long scaffolds after Zn incubationDepositorInsertIV_CS10_1033_v1
UseSynthetic BiologyAvailable SinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMTKM30
Plasmid#233502PurposeLevel 0 plasmid for MoClo, encodes inducible TDH3-tetO2p for K. marxianusDepositorInsertTDH3-tetO2p
UseSynthetic BiologyExpressionYeastMutationaddition of two tetracycline operator sequenecesAvailable SinceJuly 2, 2025AvailabilityAcademic Institutions and Nonprofits only