19,658 results

1. pRK-flag-USP19

   Plasmid

   #78597

   Purpose

   Express flag-USP19 in mammalian cells

   Depositor

   Yihong Ye

   Article

   Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

   Insert

   flag-USP19 (USP19 Human)
   

   Tags

   flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMXs-3XFLAG-EGFP-OMP25

   Plasmid

   #83354

   Purpose

   For tagging mitochondria with Sigma FLAG epitopes

   Depositor

   David Sabatini

   Article

   Chen et al Cell. 2016 Aug 25;166(5):1324-1337.e11.

   Insert

   3XFLAG-EGFP-OMP25
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Sept. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-EF1a-BbChT

   Plasmid

   #45186

   Depositor

   Dawen Cai, Joshua Sanes

   Article

   Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

   Has Service

   AAV9

   Insert

   pAAV-Ef1a-Brainbow/mCherry/mTFP-WPRE
   

   Use

   AAV; Adeno-associated viral

   Expression

   Mammalian

   Promoter

   human EF1a

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-Sport6-CD63-pHuji

   Plasmid

   #130902

   Purpose

   Expression of CD63-pHuji for visualization of multivesicular body-plasma membrane fusion.

   Depositor

   DM Pegtel

   Article

   Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

   Insert

   CD63-pHuji (CD63 Human, synthetic)
   

   Tags

   pHuji

   Expression

   Mammalian

   Mutation

   pHuji inserted between Gln-36 and Leu-37 in extra…

   Promoter

   CMV

   Available Since

   Oct. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX037

   Plasmid

   #167156

   Purpose

   MoClo-compatible Level2 vector for autonomous bioluminescence in plants encoding kanamycin resistance cassette, nnHispS, nnH3H, nnLuz and nnCPH. Does not contain NpgA.

   Depositor

   Karen Sarkisyan

   Article

   Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103

   Insert

   caffeic acid cycle genes
   

   Expression

   Plant

   Promoter

   pNOS, p35s

   Available Since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV:cTNT::Luciferase

   Plasmid

   #69915

   Purpose

   Used to package AAV9:cTNT::luciferase. Specifically transduce cardiomyocytes.

   Depositor

   William Pu

   Article

   Lin et al Circ Res. 2014 Jul 18;115(3):354-63. doi

   Insert

   Firefly luciferase
   

   Use

   AAV

   Promoter

   Chicken cardiac troponin T

   Available Since

   Nov. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pR-spondin 1

   Plasmid

   #128844

   Purpose

   bacterial expression of pR-spondin 1

   Depositor

   Marc de la Roche

   Article

   Urbischek et al Sci Rep. 2019 Apr 17;9(1):6193. doi: 10.

   Insert

   R-spondin 1 (RSPO1 Human)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-hSyn-nucGFP

   Plasmid

   #140190

   Purpose

   Overexpression of GFP with a nuclear localization sequence, under the human synapsin promoter

   Depositor

   Lorenz Studer

   Article

   Miller et al Cell Stem Cell. 2013 Dec 5;13(6):691-705

   Insert

   GFP-NLS
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-TREX1(D18N)

   Plasmid

   #27220

   Depositor

   Judy Lieberman

   Article

   Yan et al Nat Immunol. 2010 Nov . 11(11):1005-13.

   Insert

   TREX1 (TREX1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   D18N sequence 52-GAC changed to AAC, resulting i…

   Available Since

   Jan. 26, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. MSCV-Cre

    Plasmid

    #24064

    Purpose

    Retroviral coexpression of Cre and EGFP

    Depositor

    Dan Littman

    Insert

    Cre
    

    Use

    Cre/Lox and Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    MESV 5' LTR

    Available Since

    Oct. 27, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. mTFP1-pBAD

    Plasmid

    #54553

    Purpose

    Localization: Protein Expression Vector , Excitation: 462, Emission: 492

    Depositor

    Robert Campbell, Michael Davidson

    Article

    Ai et al Biochem J. 2006 Dec 15. 400(3):531-40.

    Type

    Empty backbone

    Tags

    6xHis and mTFP1

    Expression

    Bacterial

    Available Since

    July 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSLTS

    Plasmid

    #59386

    Purpose

    Expresses the lambda Red recombinase under control of the arabinose-inducible P(araB) promoter and I-SceI under control of the anhydrotetracycline-inducible P(tetA) promoter.

    Depositor

    Shelley Copley

    Article

    Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

    Type

    Empty backbone

    Use

    Genome engineering

    Expression

    Bacterial

    Available Since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. p7SL-neo-TET

    Plasmid

    #51285

    Purpose

    expresses human 7SL RNA, using its own polIII enhancer, and tagged with the self splicing intron neo cassette

    Depositor

    Astrid Roy-Engel

    Article

    Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

    Insert

    7SL RNA (RN7SL2 Human)
    

    Tags

    neoTET cassette with a tetrahymena self-splicing …

    Expression

    Mammalian

    Promoter

    7SL upstream pollII enhancer

    Available Since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. HIP-flash (HOP-flash mutant)

    Plasmid

    #83466

    Purpose

    Negative control for HOP-flash. 7xmut TEAD binding sites with minimal promoter plus luciferase reporter gene

    Depositor

    Barry Gumbiner

    Article

    Kim et al J Cell Biol. 2015 Aug 3;210(3):503-15. d

    Insert

    Multimerized mutated TEAD binding sites in the promoter of CTGF with minimal promoter (CCN2 Human)
    

    Use

    Luciferase

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHAGE-TO-dCas9-GFP

    Plasmid

    #64106

    Purpose

    Expression of Sp dCas9-GFP in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    Sp dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    sfGFP

    Expression

    Mammalian

    Promoter

    CMV-TO

    Available Since

    May 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pT7-VP1SA11

    Plasmid

    #89162

    Purpose

    Express Rotavirus SA11 VP1 in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Rotavirus SA11 VP1
    

    Expression

    Mammalian

    Promoter

    T7 promotor

    Available Since

    April 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLV-mTurquoise2-Caspase-1-mNeonGreen

    Plasmid

    #166784

    Purpose

    FRET analysis (pro caspase-1 cleavage) in living cells

    Depositor

    Hao Wu

    Article

    Magupalli et al Science. 2020 Sep 18;369(6510). pii: 369

    Insert

    mTurquoise2-caspase-1-mNeonGreen (CASP1 Human)
    

    Use

    Lentiviral

    Tags

    mNeonGreen and mTurquoise2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHAGE2 mCherry-Rtn4a

    Plasmid

    #86683

    Purpose

    Lentivirus to stably express fluorescent human protein in mammalian cells

    Depositor

    Tom Rapoport

    Article

    Wang et al Elife. 2016 Sep 13;5. pii: e18605. doi:

    Insert

    Rtn4a (RTN4 Human)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. Dronpa3-C1

    Plasmid

    #54655

    Purpose

    Localization: C1 Cloning Vector, Excitation: 503, Emission: 518

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Tags

    Dronpa3

    Expression

    Mammalian

    Available Since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

20. mEmerald-FilaminA-N-9

    Plasmid

    #54098

    Purpose

    Localization: Actin/Focal Adhesions, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    FilaminA (FLNA Human)
    

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    D2626H

    Promoter

    CMV

    Available Since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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