We narrowed to 11,278 results for: Vars;

1. pDEST14-DoubleCatcher-⍺-Lock

   Plasmid

   #216286

   Purpose

   Expresses DoubleCatcher-⍺-Lock in bacterial cells. DoubleCatcher-⍺-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   DoubleCatcher ⍺-Lock
   

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDEST14-DoubleCatcher-β-Lock

   Plasmid

   #216287

   Purpose

   Expresses DoubleCatcher-β-Lock in bacterial cells. DoubleCatcher-β-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   DoubleCatcher β-Lock
   

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDEST14-DoubleCatcher-γ-Lock

   Plasmid

   #216288

   Purpose

   Expresses DoubleCatcher-γ-Lock in bacterial cells. DoubleCatcher-γ-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   DoubleCatcher γ-Lock
   

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDEST14-DoubleCatcher-δ-Lock

   Plasmid

   #216289

   Purpose

   Expresses DoubleCatcher-δ-Lock in bacterial cells. DoubleCatcher-δ-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   DoubleCatcher δ-Lock
   

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST14-DoubleCatcher-ε-Lock

   Plasmid

   #216290

   Purpose

   Expresses DoubleCatcher-ε-Lock in bacterial cells. DoubleCatcher-ε-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   DoubleCatcher ε-Lock
   

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL2_167

   Plasmid

   #199099

   Purpose

   Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

   Depositor

   Charlie Gilbert, Nili Ostrov, Cultivarium Tools

   Article

   Gilbert et al bioRxiv 2023.05.19.541510

   Insert

   pBBR1 ORI plus NGS barcode 13, gentamicin resistance cassette, mScarlet-I cassette, oriT
   

   Use

   Synthetic Biology

   Available Since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL2_170

   Plasmid

   #199102

   Purpose

   Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

   Depositor

   Charlie Gilbert, Nili Ostrov, Cultivarium Tools

   Article

   Gilbert et al bioRxiv 2023.05.19.541510

   Insert

   pSa ORI plus NGS barcode 16, gentamicin resistance cassette, mScarlet-I cassette, oriT
   

   Use

   Synthetic Biology

   Available Since

   Sept. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTlpA-Wasabi-NF

   Plasmid

   #86100

   Purpose

   Nonfluorescent variant of mWasabi reporter gene gated by wild type TlpA

   Depositor

   Mikhail Shapiro

   Article

   Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

   Inserts

   TlpA
   mWasabi
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   D341A and S71T, G73A

   Promoter

   TlpA and TlpA, LacI

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. LENG

   Plasmid

   #111162

   Purpose

   miR-E (miR-30 variant)-based RNAi with GFP reporter

   Depositor

   Johannes Zuber

   Article

   Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   GFP

   Available Since

   May 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. eNme2C-CBE6a

    Plasmid

    #215829

    Purpose

    Express CBE6a (with eNme2-C variant) in mammalian cells

    Depositor

    David Liu

    Article

    Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

    Insert

    CBE6a-eNme2C-2xUGI
    

    Expression

    Mammalian

    Available Since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDB007ns2a

    Plasmid

    #99214

    Purpose

    Accessory plasmid for PACE of PylRS variants; negative selection

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Ppsp [SD8] gIII; PproK tyrT(Opt,CUA); Ptet [SD4] T7RNAP(S12*,S203*)
    

    Mutation

    amber codons at positions 12 and 203 of T7 RNA po…

    Promoter

    Ppsp, PproK, and Ptet

    Available Since

    Oct. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDB038a

    Plasmid

    #99211

    Purpose

    Accessory plasmid for PACE of PylRS variants

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Ppsp [SD8] gIII(P29*,Y184*), luxAB; PproK pylT
    

    Expression

    Bacterial

    Mutation

    amber codons at positions 29 and 184 of gIII

    Promoter

    Ppsp and PproK

    Available Since

    Oct. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. GFP-Paxillin DN

    Plasmid

    #186153

    Purpose

    For expressing paxillin variants in mammalian cells

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    PXN (PXN Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    Paxilln residues 1-321 added to N-terminus of WT …

    Promoter

    cmv

    Available Since

    July 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDisplay-Gncp-iGluSnFR

    Plasmid

    #107337

    Purpose

    Noncircularly permutated (ncp) variant of iGluSnFR. Membrane-displayed.

    Depositor

    Robert Campbell

    Article

    Wu et al ACS Chem Biol. 2018 Jan 22. doi: 10.1021

    Insert

    GltI based and noncircularly permutated glutamate biosensor
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYC56

    Plasmid

    #63910

    Purpose

    Recipient integrative vector to construct mCherry tagged fusions

    Depositor

    Irene Castano Navarro

    Article

    Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

    Type

    Empty backbone

    Tags

    mCherry

    Expression

    Yeast

    Available Since

    April 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDB038b

    Plasmid

    #99212

    Purpose

    Accessory plasmid for PACE of PylRS variants

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Ppsp [SD8] gIII(P29*,P83*,Y184*), luxAB; PproK pylT
    

    Expression

    Bacterial

    Mutation

    amber codons at positions 29, 83, and 184 of gIII

    Promoter

    Ppsp and PproK

    Available Since

    Oct. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGL2_176

    Plasmid

    #199108

    Purpose

    Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

    Depositor

    Charlie Gilbert, Nili Ostrov, Cultivarium Tools

    Article

    Gilbert et al bioRxiv 2023.05.19.541510

    Insert

    p15A ORI plus NGS barcode 24, gentamicin resistance cassette, mScarlet-I cassette, oriT
    

    Use

    Synthetic Biology

    Available Since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGL2_169

    Plasmid

    #199101

    Purpose

    Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

    Depositor

    Charlie Gilbert, Nili Ostrov, Cultivarium Tools

    Article

    Gilbert et al bioRxiv 2023.05.19.541510

    Insert

    pIJ101 ORI plus NGS barcode 15, gentamicin resistance cassette, mScarlet-I cassette, oriT
    

    Use

    Synthetic Biology

    Available Since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. CMV-mito-CitroffRH

    Plasmid

    #158548

    Purpose

    Mitochondria-targeted nonbinding variant of inverse-response citrate sensor

    Depositor

    Robert Campbell

    Article

    Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

    Insert

    CitroffRH
    

    Expression

    Mammalian

    Promoter

    cmv

    Available Since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. mFGF8a

    Plasmid

    #22088

    Depositor

    Gail Martin

    Article

    Crossley et al Development. 1995 Feb . 121(2):439-51.

    Insert

    FGF8 (Fgf8 Mouse)
    

    Use

    Bacterial

    Available Since

    Sept. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

21. pSC101-GFPmut2-mScarlet-I TGATC

    Plasmid

    #208190

    Purpose

    nonsense mistranslation, TGA stop variant 2

    Depositor

    Tanel Tenson

    Article

    Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

    Inserts

    GFPmut2
    mScarlet-I
    

    Use

    Reporter

    Expression

    Bacterial

    Mutation

    Insertion 6TGA*(stop) 7TCT(Ser)

    Promoter

    Ptet+dnaK P1

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. pGL2_173

    Plasmid

    #199105

    Purpose

    Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

    Depositor

    Charlie Gilbert, Nili Ostrov, Cultivarium Tools

    Article

    Gilbert et al bioRxiv 2023.05.19.541510

    Insert

    pBBR1-UP ORI plus NGS barcode 21, gentamicin resistance cassette, mScarlet-I cassette, oriT
    

    Use

    Synthetic Biology

    Available Since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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23. pET30b_T7Sav

    Plasmid

    #138588

    Purpose

    T7-tagged streptavidin with native C-terminus controlled by PT7 promoter; KanR, pBR322 ori

    Depositor

    Markus Jeschek

    Article

    Jeschek et al Metab Eng. 2017 Mar;40:33-40. doi: 10.10

    Insert

    T7-tagged streptavidin variant with native C-terminus
    

    Use

    Synthetic Biology

    Tags

    T7-tag

    Expression

    Bacterial

    Promoter

    PT7

    Available Since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. peCALWY-NB

    Plasmid

    #22239

    Depositor

    Maarten Merkx

    Article

    Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

    Insert

    eCALWY non-binding variant
    

    Expression

    Mammalian

    Mutation

    C284S, C287S, C413S and C416S mutations linker c…

    Available Since

    Jan. 11, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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25. pTrans_9xD3B_Cluc_Norm

    Plasmid

    #68415

    Purpose

    CRISPR-Display Cypridina Luciferase-2A-Venus YFP "normalizer," transient format.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    Cypridina Luciferase-2A-Venus YFP
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    YFP: M153T,V163A,S175G ("Venus") YFP Va…

    Promoter

    minimalCMV

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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26. pCAG-GFPmG1

    Plasmid

    #50444

    Purpose

    Expression of a EGFP variant in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

    Insert

    GFPmG1
    

    Expression

    Mammalian

    Mutation

    E143K, N147Q

    Promoter

    CAG

    Available Since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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27. pEYFP-N1-HsSmg7_H

    Plasmid

    #146476

    Purpose

    Mammalian Expression of HsSmg7

    Depositor

    Elisa Izaurralde

    Article

    Unterholzner et al Mol Cell. 2004 Nov 19. 16(4):587-96.

    Insert

    HsSmg7 (SMG7 Human)
    

    Expression

    Mammalian

    Mutation

    one non silent mutation G2560A (V854I) and three …

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGL2_168

    Plasmid

    #199100

    Purpose

    Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

    Depositor

    Charlie Gilbert, Nili Ostrov, Cultivarium Tools

    Article

    Gilbert et al bioRxiv 2023.05.19.541510

    Insert

    RSF1010 ORI plus NGS barcode 14, gentamicin resistance cassette, mScarlet-I cassette, oriT
    

    Use

    Synthetic Biology

    Available Since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGBT-RP2-1

    Plasmid

    #31828

    Depositor

    Stephen Ekker

    Article

    Clark et al Nat Methods. 2011 Jun;8(6):506-15. Epub

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    GFP GM2 variant and mRFP

    Promoter

    beta Actin

    Available Since

    Aug. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. cpLOV2-V5

    Plasmid

    #168991

    Purpose

    circularly permuted LOV2 variant-V5

    Depositor

    Yubin Zhou

    Article

    He et al Nat Chem Biol. 2021 Aug;17(8):915-923. d

    Insert

    cpLOV2-V5
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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