Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 12,157 results for: pcdna

Showing: 6261 - 6280 of 12157 results

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pcDNA3.1-SLC25A46[∆2-83]-P2A-Electra1 (pAH14)

Plasmid

#227619

Purpose

Overexpression of SLC25A46 lacking its N-terminal region in mammalian cell lines

Depositor

Luke Chao

Article

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

SLC25A46 (SLC25A46 Human)

Tags

P2A-mElectra1

Expression

Mammalian

Mutation

deleted aa 2-83

Promoter

CMV, T7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA4TO-SCN8A-Variant-3-IRES-mScarlet

Plasmid

#209411

Purpose

Expression of wild-type adult isoform of human SCN8A.

Depositor

Al George

Article

Vanoye et al JCI Insight. 2024 May 21;9(12):e177530.

Insert

SCN8A Variant 3, stabilized (SCN8A Human)

Expression

Mammalian

Mutation

Modifed human HSPA5 intron 4 inserted after bp237…

Promoter

CMV

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-FLAG-Regnase-3-HA_full-length

Plasmid

#222656

Purpose

Mammalian expression vector to express full-length Regnase-3 tagged with FLAG at N-term and HA at C-term

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12c (Zc3h12c Mouse)

Tags

FLAG and HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-FLAG-Regnase-3_1-547 aa

Plasmid

#222657

Purpose

Mammalian expression vector to express 1-547 aa of Regnase-3 tagged with FLAG at N-term

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12c 1-547 aa (Zc3h12c Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

mutated histidine 548 to a stop codon

Promoter

CMV

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-FLAG-Regnase-3_1-409 aa

Plasmid

#222658

Purpose

Mammalian expression vector to express 1-409 aa of Regnase-3 tagged with FLAG at N-term

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12c 1-409 aa (Zc3h12c Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

mutated proline 410 to a stop codon

Promoter

CMV

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-FLAG-Regnase-3_1-244 aa

Plasmid

#222659

Purpose

Mammalian expression vector to express 1-244 aa of Regnase-3 tagged with FLAG at N-term

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12c 1-244 aa (Zc3h12c Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

mutated asparagine 245 to a stop codon

Promoter

CMV

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-SV40NLS-OgeuIscBE193A-3XHA

Plasmid

#222861

Purpose

This plasmid codes for the OgeuIscB nickase

Depositor

Vincent Dion

Article

Mangin et al bioRxiv 2023.10.24.563270

Insert

OgeuIscB Nickase

Use

CRISPR

Tags

3X HA and Nucleoplasmin NLS and ITR2

Expression

Mammalian

Mutation

E193A for nickase mutation

Promoter

CMV

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc-GFP-TNRC6A-SD-CIM1-WT

Plasmid

#215898

Purpose

Expression of TNRC6A-CIM1 domain

Depositor

Kumiko Ui-Tei

Article

Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

Insert

TNRC6A (TNRC6A Human)

Tags

myc-GFP

Expression

Mammalian

Mutation

contain aa1224-1528

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc-GFP-TNRC6A-SD-CIM2-WT

Plasmid

#215899

Purpose

Expression of TNRC6A-CIM2 domain

Depositor

Kumiko Ui-Tei

Article

Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

Insert

TNRC6A (TNRC6A Human)

Tags

myc-GFP

Expression

Mammalian

Mutation

contain aa1529-1709

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-mGFP-FXR1a-CC1-CC1

Plasmid

#225458

Purpose

Express EGFP-fusion protein of FXR1 isoform a (CC1-CC1)

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

FXR1 isoform a (FXR1 Human)

Tags

EGFP

Expression

Mammalian

Mutation

CC2 replaced with a second copy of CC1

Promoter

CMV

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-mGFP-FXR1a-CC2mut (V361P)

Plasmid

#225456

Purpose

Express mEGFP-fusion protein of FXR1 isoform a (V361P)

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

FXR1 isoform a (FXR1 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

V361P

Promoter

CMV

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-mGFP-FMR1iso1-CC2mut (V427P)

Plasmid

#225466

Purpose

Express mEGFP-fusion protein of FMR1 isoform 1 (V427P)

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

FMR1 isoform 1 (FMR1 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

V427P

Promoter

CMV

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-mGFP-FXR1a-CC2-CC2

Plasmid

#225459

Purpose

Express mEGFP-fusion protein of FXR1 isoform a (CC2-CC2)

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

FXR1 isoform a (FXR1 Human)

Tags

mEGFP

Expression

Mammalian

Mutation

CC1 replaced with a second copy of CC2

Promoter

CMV

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3puro-EGFP-GAPDH-Tudor-R rich

Plasmid

#225478

Purpose

Express mEGFP-GAPDH-Tudor-R fusion

Depositor

Christine Mayr

Article

Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

Insert

GAPDH fused with Tudor and R-rich regions of TDRD3 (GAPDH Human)

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) EGFP-Rac1(G12C, K96H)

Plasmid

#224281

Purpose

Cell transfection and expression of Rac1 (G12C, K96H)

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

Rac1 (RAC1 Human)

Expression

Mammalian

Mutation

G12C, K96H

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) EGFP-Rap1A(G12C, L96F)

Plasmid

#224277

Purpose

Cell transfection and expression of Rap1A (G12C, L96F)

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

Rap1A (RAP1A Human)

Expression

Mammalian

Mutation

G12C, L96F

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Myc-BirA*-RNF8-C403S

Plasmid

#221685

Purpose

Mammalian expression of Myc-tagged BirA*-RNF8 (C403S) fusion protein for BioID

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

RNF8 (RNF8 Human)

Expression

Mammalian

Mutation

C403S

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Myc-BirA*-RNF8-R42A

Plasmid

#221684

Purpose

Mammalian expression of Myc-tagged BirA*-RNF8 (R42A) fusion protein for BioID

Depositor

Derrick Sek Tong Ong

Article

Chuah et al Cell Death Differ. 2023 Aug;30(8):1973-1

Insert

RNF8 (RNF8 Human)

Expression

Mammalian

Mutation

R42A

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-TRBP-L326A/V336A/H338A

Plasmid

#215894

Purpose

Expression of TRBP-L326A/V336A/H338A

Depositor

Kumiko Ui-Tei

Article

Komori et al Biol Open. 2020 Feb 25;9(2):bio050435. d

Insert

TRBP-L326A/V336A/H338A (TARBP2 Human)

Expression

Mammalian

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-TRBP-V336A/H338A/Y358A

Plasmid

#215893

Purpose

Expression of TRBP-V336A/H338A/Y358A

Depositor

Kumiko Ui-Tei

Article

Komori et al Biol Open. 2020 Feb 25;9(2):bio050435. d

Insert

TRBP-V336A/H338A/Y358A (TARBP2 Human)

Expression

Mammalian

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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Showing: 6261 - 6280 of 12157 results

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