We narrowed to 11,748 results for: pcdna

1. pcDNA3.1(+)/CUG-nLuc-3XFLAG-PEST

   Plasmid

   #127313

   Purpose

   Express 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from CUG start codon

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Use

   Tags

   3xFLAG and PEST

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3.1(+)/GGG-stop-nLuc-3XFLAG

   Plasmid

   #127311

   Purpose

   Express 3xFLAG tagged nanoLuciferase (nLuc) from negative control GGG start codon, but translation terminated immediately by stop codon

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1(+)/AAA-stop-nLuc-3XFLAG

   Plasmid

   #127310

   Purpose

   Express 3xFLAG tagged nanoLuciferase (nLuc) from negative control AAA start codon, but translation terminated immediately by stop codon

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3-Flag-RNF125 Ring Mutant

   Plasmid

   #122046

   Purpose

   express RNF125 RM

   Depositor

   Ze'ev Ronai

   Insert

   RNF125 RM (RNF125 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Ring mutant C37A, C40A

   Promoter

   Available since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. ELK1 A box-NLS pcDNA3.1/HisC

   Plasmid

   #121096

   Purpose

   Expression of the A box fragment of ELK1

   Depositor

   Paul Herring

   Article

   Zhou et al Mol Cell Biol. 2005 Nov . 25(22):9874-85

   Insert

   ELK1 (Elk1 Mouse)
   

   Use

   Tags

   6XHis-Omni-xpress

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. ELK1 B box-NLS pcDNA3.1/HisC

   Plasmid

   #121095

   Purpose

   Expression of the B box fragment of ELK1

   Depositor

   Paul Herring

   Article

   Zhou et al Mol Cell Biol. 2005 Nov . 25(22):9874-85

   Insert

   ELK1 (Elk1 Mouse)
   

   Use

   Tags

   6XHis-Omni-xpress

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA5-puro-eGFP-HEC1-d80

   Plasmid

   #114025

   Purpose

   Inducible expression of eGFP-tagged HEC1-d80 in FLPin Trex cell lines

   Depositor

   Geert Kops

   Article

   Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

   Insert

   eGFP-d80-HEC1 (NDC80) (NDC80 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Hec1 - 1 to 80 deleted

   Promoter

   Available since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA5-hygro-eGFP-HEC1-d80

   Plasmid

   #114030

   Purpose

   Inducible expression of eGFP-tagged HEC1-d80 in FLPin Trex cell lines

   Depositor

   Geert Kops

   Article

   Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

   Insert

   eGFP-d80-HEC1 (NDC80) (NDC80 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Hec1 - 1 to 80 deleted

   Promoter

   Available since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA5-hygro-eGFP-HEC1-8A/S15D

   Plasmid

   #114031

   Purpose

   Inducible expression of eGFP-tagged HEC1-8A/S15D in FLPin Trex cell lines

   Depositor

   Geert Kops

   Article

   Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

   Insert

   eGFP-HEC1-8A/S15D (NDC80) (NDC80 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Hec1 - 8A/S15D

   Promoter

   Available since

   Oct. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CoxVIIIx2-CeNL(Ca2+)_19μ-pcDNA3

    Plasmid

    #111923

    Purpose

    Cyan color luminescent indicator for calcium signaling.Mitochondrial targeting signals of subunit VIII of human cytochrome c oxidase (CoxVIII) were fused at the N-terminus of CeNL(Ca2+)_19μ

    Depositor

    Takeharu Nagai

    Article

    Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

    Insert

    CeNL(Ca2+)_19μ
    

    Use

    Tags

    CoxVIIIx2

    Expression

    Mammalian

    Mutation

    E67D, F92W, E104D, D133E at CaM

    Promoter

    CMV

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA5-hygro-eGFP-HEC1-d207

    Plasmid

    #114051

    Purpose

    Expression of exogenous eGFP HEC1 delta 207

    Depositor

    Geert Kops

    Article

    Nijenhuis et al J Cell Biol. 2013 Apr 15;201(2):217-31.

    Insert

    eGFP-HEC1-d207 (NDC80 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HEC1 delta 207

    Promoter

    Available since

    Sept. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA5-hygro-eGFP-STOP-HEC1

    Plasmid

    #114026

    Purpose

    Inducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines. Due to insertion of a STOP-codon after the eGFP-tag, this plasmid only expressed eGFP.

    Depositor

    Geert Kops

    Article

    Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

    Insert

    eGFP-STOP-HEC1 (NDC80 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Start codon of Hec1 is mutated to stop codon

    Promoter

    Available since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CoxVIIIx2-CeNL(Ca2+)_1.2μ-pcDNA3

    Plasmid

    #111922

    Purpose

    Cyan color luminescent indicator for calcium signaling.Mitochondrial targeting signals of subunit VIII of human cytochrome c oxidase (CoxVIII) were fused at the N-terminus of CeNL(Ca2+)_1.2μ

    Depositor

    Takeharu Nagai

    Article

    Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

    Insert

    CeNL(Ca2+)_1.2μ
    

    Use

    Tags

    CoxVIIIx2

    Expression

    Mammalian

    Mutation

    E67D, E104D, D133E at CaM

    Promoter

    CMV

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA5-FRT-TetO-Snu114-fSNAPc

    Plasmid

    #106989

    Purpose

    open reading frame of Snu114 inserted into pcDNA5-FRT-TetO-fSNAPc using the KpnI and NotI restriction sites

    Depositor

    Melissa Moore

    Article

    Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

    Insert

    Snu114
    

    Use

    Tags

    fSNAP

    Expression

    Mutation

    Promoter

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA-Flag-Sesn-C mSesn2 (delAB)

    Plasmid

    #111799

    Purpose

    Mammalian Expression of mSesn2

    Depositor

    Jun Hee Lee

    Article

    Ro et al FEBS J. 2014 Sep;281(17):3816-27. doi: 1

    Insert

    mSesn2 (Sesn2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    AB domain truncation

    Promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA-Flag-Sesn-B+C mSesn2 (delA)

    Plasmid

    #111798

    Purpose

    Mammalian Expression of mSesn2

    Depositor

    Jun Hee Lee

    Article

    Ro et al FEBS J. 2014 Sep;281(17):3816-27. doi: 1

    Insert

    mSesn2 (Sesn2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    A domain truncation

    Promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA-Flag-Sesn-A mSesn2 (delBC)

    Plasmid

    #111797

    Purpose

    Mammalian Expression of mSesn2

    Depositor

    Jun Hee Lee

    Article

    Ro et al FEBS J. 2014 Sep;281(17):3816-27. doi: 1

    Insert

    mSesn2 (Sesn2 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    BC domain truncation

    Promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PCDNA3-N3FLAG-TAF12-HFD(50-130)

    Plasmid

    #105615

    Purpose

    transient express TAF12 HFD with 3*FLAG tag at N terminal in HEK 293T cells

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    TAF12-HFD amino acid 50-130 with FLAG tag (Taf12 Mouse)
    

    Use

    Tags

    3*FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PCDNA3-TADA1-HFD(140-230)

    Plasmid

    #105984

    Purpose

    transient express TADA1-HFD fragment in HEK 293T cells

    Depositor

    Christopher Vakoc

    Article

    Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

    Insert

    TADA1-HFD (140-230) (Tada1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-HA-REDD1 N181-211 (#595)

    Plasmid

    #99939

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

    Insert

    REDD1 (DDIT4 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    delAA181-211insNAAIRS linker

    Promoter

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge