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pET15b-Fab1 (Francisella tularensis)

Plasmid

#61690

Purpose

Inducible expression of the FabI enzyme (enoyl reductase) from Francisella tularensis

Depositor

Michael Johnson

Article

Hevener et al J Med Chem. 2012 Jan 12;55(1):268-79. do

Insert

enoyl-[acyl-carrier-protein] reductase (NADH)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 10, 2015

Availability

Academic Institutions and Nonprofits only
3294_pETcon-SARS2-RBD_Omicron-BA1

Plasmid

#184408

Purpose

yeast surface display of the SARS-CoV-2 Omicron BA.1 variant RBD

Depositor

Jesse Bloom

Article

Starr et al Science. 2022 Jun 28:eabo7896. doi: 10.1

Insert

SARS-CoV-2 Omicron BA.1 Spike receptor binding domain (RBD) (S SARS-CoV-2 virus)

Use

Tags

HA and c-Myc

Expression

Yeast

Mutation

G339D+S371L+S373P+S375F+K417N+N440K+G446S+S477N+T…

Promoter

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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Pet22b-mCherry-TEAD4-IDR

Plasmid

#166442

Purpose

Bacterial expression of 6x His tagged mCherry-TEAD4 -IDR fusion protein

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mCherry, TEAD4-IDR (TEAD4 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 7, 2021

Availability

Academic Institutions and Nonprofits only

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pET-CH2 (1192-1318)

Plasmid

#99338

Purpose

Bacterial expression of mouse CBP CH2 domain

Depositor

Peter Wright

Article

Park et al FEBS Lett. 2013 Aug 19;587(16):2506-11.

Insert

CBP CH2 (1192-1318) (Crebbp Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pET15b/GgVcl V1-851 (aka Vh)

Plasmid

#46172

Depositor

Susan Craig

Article

Cohen et al J Biol Chem. 2005 Apr 29;280(17):17109-1

Insert

Vcl 1-851 (VCL Chicken)

Use

Tags

His

Expression

Bacterial

Mutation

aa 1-851 (aka Vh) head domain

Promoter

T7

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pET11a-DAAT-V33G/T242G

Plasmid

#159278

Purpose

Aminotransferase variant with near-native catalytic efficiency toward D-tryptophan

Depositor

Roberto Chica

Article

Parmeggiani et al ACS Catalysis 9, 3482–3486.

Insert

Engineered d-alanine aminotransferase

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Valine 33 and threonine 242 have been mutated to …

Promoter

T7

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pETM6-At4CL-PhCHS-MsCHI

Plasmid

#73392

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

At4CL
PhCHS
MsCHI

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

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pET28A-p53 (1-73) (P12A P13A)

Plasmid

#62066

Purpose

Expression of human p53 (residues 1-73) (P12A, P13A) in e. coli

Depositor

Gary Daughdrill

Article

Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

Insert

p53 (TP53 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Contains TAD residues 1-73; P12A, P13A

Promoter

T7

Available since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only
pET21a-mAIM2-deltaPYD-GST

Plasmid

#51534

Purpose

Express GST-tagged HIN domain of mAIM2 in bacteria

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

Aim2 (Aim2 Mouse)

Use

Tags

GST and T7

Expression

Bacterial

Mutation

Promoter

lac operator

Available since

March 11, 2014

Availability

Academic Institutions and Nonprofits only

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pETM6-Vv4CL-CmCHS-MsCHI

Plasmid

#73402

Purpose

Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

Depositor

Mattheos Koffas

Article

Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

Inserts

Vv4CL
CmCHS
MsCHI

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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