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pHIS3p:mRuby2-Tub1+3'UTR::LEU2

Plasmid

#50645

Depositor

Wei-Lih Lee

Article

Markus et al Traffic. 2015 Jul;16(7):773-86. doi: 10.

Insert

mRuby2-Tub1

Use

Yeast expression

Tags

mRuby2

Promoter

HIS3p

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW ymYPET

Plasmid

#118455

Purpose

Overexpression of ymYPET

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

ymYPET

Expression

Yeast

Mutation

YPET A206K, F208S, E232L, N235D

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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GPI-Gamillus

Plasmid

#197914

Purpose

Monomer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Petrich et al PLoS One. 2023 Aug 3;18(8):e0285486. doi

Insert

glycosylphosphatidylinositol-Gamillus

Expression

Mammalian

Mutation

none

Available Since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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LSSmKate2-N1

Plasmid

#54796

Purpose

Localization: N1 Cloning Vector, Excitation: 460, Emission: 605

Depositor

Michael Davidson, Vladislav Verkhusha

Article

Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Type

Empty backbone

Tags

LSSmKate2

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pBSV2_2

Plasmid

#118226

Purpose

Empty E. coli - B. burgdorferi shuttle vector; kanamycin resistant

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pEB1-Sapphire

Plasmid

#103976

Purpose

Plasmid encoding Sapphire

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

Sapphire

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (S72A, Y145F,…

Promoter

proC

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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p{Mhc-F4-678-DsRed::GW,attB}

Plasmid

#182171

Purpose

fluorescent reporter gene with intron containing Gateway cloning cassette

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

DsRed

Expression

Insect

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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pPEPX

Plasmid

#122631

Purpose

BglFusion compatible integration vector for Streptococcus pneumoniae

Depositor

Jan-Willem Veening

Article

Keller et al Genes (Basel). 2019 May 22;10(5). pii: g

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pEB2-2ndVal mKate2

Plasmid

#104030

Purpose

Plasmid encoding 2ndVal mKate2

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

2ndVal mKate2

Use

Low copy

Expression

Bacterial

Mutation

mKate2 with valine inserted in 2nd position

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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LIIbeta F 1-2 RNAi

Plasmid

#54403

Depositor

Martin Parniske

Article

Binder et al PLoS One. 2014 Feb 13;9(2):e88218. doi:

Type

Empty backbone

Use

Plant expression

Available Since

Dec. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW yosfGFP-T2A-mCherry

Plasmid

#118448

Purpose

Produces equimolar expression of yeast codon-optimized sfGFP and mCherry

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

yosfGFP-T2A-mCherry

Expression

Yeast

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA4TO-sfGFP-24xGCN4_v1

Plasmid

#61056

Purpose

A direct fusion of sfGFP to the 24xGCN4_v1 peptide array

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

sfGFP-24xGCN4_v1 peptide array

Expression

Mammalian

Available Since

Nov. 17, 2014

Availability

Academic Institutions and Nonprofits only
p{Mhc-F4-678-eGFP::FRT,attB}

Plasmid

#182185

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

EGFP

Expression

Insect

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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p{ie1-DsRed::FRT-RC,attB}

Plasmid

#182191

Purpose

fluorescent reporter gene with yeast recombination site within intron

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

Insert

DsRed

Expression

Insect

Available Since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pSPB845

Plasmid

#128296

Purpose

asymmetric plasmid partitioning E. coli - regulatory plasmid

Depositor

Matthew Bennett

Article

Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

Insert

PLlacO1 - lp-cfp-parB, BBa_J23110 - lacI

Expression

Bacterial

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pLRD04

Plasmid

#208258

Purpose

Production of ent-kaurene in E. coli MEV20

Depositor

Christopher Voigt

Article

Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

Inserts

tHMGR
ptmT2
blr2150

Expression

Bacterial

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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mTagRFP-T2-Farnesyl-5

Plasmid

#58037

Purpose

Localization: Plasma Membrane, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Insert

Farnesyl (HRAS Human)

Tags

mTagRFP

Expression

Mammalian

Mutation

encodes final 20 aa of NM_001130442.1

Promoter

CMV

Available Since

Nov. 4, 2014

Availability

Industry, Academic Institutions, and Nonprofits
SJZ7-KAN

Plasmid

#228260

Purpose

Template plasmid for yeast gene knockout, confers antibiotic resistance to G418

Depositor

Zhiping Xie

Article

Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

Insert

kan

Expression

Yeast

Promoter

GPM1 S.c.

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pDRESS_mTurquoise2_spatial-linker-P2A_mScarletI

Plasmid

#130510

Purpose

expresses mScarlet1

Depositor

Dorus Gadella

Article

Bindels et al Nat Protoc. 2020 Feb;15(2):450-478. doi:

Insert

mScarlet1

Expression

Mammalian

Available Since

Nov. 7, 2019

Availability

Academic Institutions and Nonprofits only

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CYPHR

Plasmid

#14864

Depositor

Alexandra Newton

Article

Violin et al J Cell Biol. 2003 Jun 9. 161(5):899-909.

Insert

cyan/yellow PH domain reporter (Plcd1 )

Tags

CFP and YFP

Expression

Mammalian

Available Since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only