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171,784 results

Showing: 6301 - 6320 of 171784 results

  6. 14UAS PSD95:GFP 5UAS DSRedExpress

    Plasmid
    #74315
    Purpose
    Encodes a PSD95:GFP fusion protein and DsRedExpress from separate UAS cassettes; for long-term two-photon time-lapse imaging of dendrite growth and synaptogenesis in zebrafish
    Depositor
    Martin Meyer, Stephen Smith
    Article
    Niell et al Nat Neurosci. 2004 Mar;7(3):254-60. Epub
    Inserts
    zebrafish PSD95
    DsRedExpress
    Tags
    EGFP
    Mutation
    Y599H and I793S (please see depositor comment bel…
    Available Since
    May 19, 2016
    Availability
    Academic Institutions and Nonprofits only

  8. TFORF0983

    Plasmid
    #143739
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    KLF5 (KLF5 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Sept. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    143739-plasmid-map-sequence-id-407888

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  10. pCAS

    Plasmid
    #60847
    Purpose
    Expresses S. pyogenes Cas9 plus an HDV ribozyme-sgRNA for genome editing in yeast
    Depositor
    Jamie Cate
    Article
    Ryan et al Elife. 2014 Aug 19;3. doi: 10.7554/eLife
    Inserts
    S. pyogenese Cas9
    RNA pol III promoter (tRNA-Tyr)
    hepatitis delta virus ribozyme, genomic
    sgRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    NLS/His8 and TTT 3' extension prior to sgRNA
    Expression
    Bacterial and Yeast
    Mutation
    L4 is UUCG tetraloop and guide targets LYP1 (CATA…
    Available Since
    Jan. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    60847-plasmid-map-sequence-id-197511

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  15. MADR pDonor-H3F3A-G34R-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

    Plasmid
    #131463
    Purpose
    MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic H3F3A G34R, PDGFRA D842V, and TRP53
    Depositor
    Joshua Breunig
    Article
    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do
    Insert
    H3F3A-G34R-EGFP AU1 pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
    Use
    Cre/Lox and Synthetic Biology; Mosaic analysis fo…
    Tags
    Trp53-V5, H3F3A-EGFP-AU1
    Expression
    Mammalian
    Mutation
    H3F3A G34R, Pdgfra D842V
    Promoter
    none (4x polyA to mitigate episomal expression)
    Available Since
    July 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    131463-plasmid-map-sequence-id-305001

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  17. p_m16S

    Plasmid
    #160549
    Purpose
    Used for mycoplasma detection by qPCR. 16S insert truncated internally from the strain Mycoplasma capricolum subsp. capricolum strain California Kid. ATB: Carbenicilline
    Depositor
    Denis Gerlier
    Article
    Jean et al PLoS One. 2017 Feb 22;12(2):e0172358. do
    Insert
    16S rDNA fragment from M. capricolum subsp. capricolum strain California Kid (gi_83283139)
    Use
    Unspecified
    Available Since
    Dec. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    160549-plasmid-map-sequence-id-319414

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  18. AICSDP-42:AAVS1-mTagRFPT-CAAX

    Plasmid
    #107580
    Purpose
    Homology arms, promoter and linker-mTagRFPT-CAAX sequence for internal insertion of CAGGS-driven mTagRFPT-CAAX at the AAVS1 safe harbor location in human cells
    Depositor
    Allen Institute for Cell Science
    Insert
    AAVS1 Homology Arms with CAGGS-driven mTagRFPT-CAAX (AAVS1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    May 9, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    107580-plasmid-map-sequence-id-208159

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  20. P362-FBP_12

    Plasmid
    #139703
    Purpose
    T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway
    Depositor
    Daniel Voytas
    Article
    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1
    Insert
    pSlH4:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pAtuMas:NhH3H:tAtuMas, p35S:NnHisps:t35S, p35S:NnLuz:AtuOcs
    Use
    Luciferase
    Expression
    Plant
    Promoter
    various
    Available Since
    May 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    139703-plasmid-map-sequence-id-296521

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Showing: 6301 - 6320 of 171784 results