We narrowed to 8,358 results for: BLI

1. Hs.KRAS4b G12V

   Plasmid

   #83132

   Purpose

   Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12V mutation

   Depositor

   Dominic Esposito

   Insert

   KRAS (KRAS Human)
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   G12V

   Promoter

   Available since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pQTEV-CIP29

   Plasmid

   #34822

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

   Insert

   cytokine induced protein 29 kDa (SARNP Human)
   

   Use

   Tags

   His and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. CD44Z-c011

   Plasmid

   #175455

   Purpose

   Protein expression/secretion in mammalian cells. CD44s (standard form; exons 2-5, 15-16), A20-E649.

   Depositor

   Opher Gileadi

   Insert

   CD44 (CD44 Human)
   

   Use

   Tags

   His6

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. qMaLioffG

   Plasmid

   #212825

   Purpose

   green fluorescent FLIM indicator for endogenous ATP imaging

   Depositor

   Tetsuya Kitaguchi

   Article

   Arai et al bioRxiv 2023.08.29.555275

   Insert

   qMaLioffG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQTEV-PSMD11

   Plasmid

   #31333

   Depositor

   Konrad Buessow

   Article

   Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

   Insert

   proteasome (prosome, macropain) 26S subunit, non-ATPase, 11 (PSMD11 Human)
   

   Use

   Tags

   His and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. p5'Entry-lck-5.5kb

   Plasmid

   #58890

   Purpose

   provides zebrafish lck promoter (5.5 kb) for GateWay construction of Tol2 transgenes

   Depositor

   Jeffrey Yoder

   Insert

   zebrafish lymphocyte-specific protein tyrosine kinase (lck) promoter, 5.5 kb
   

   Use

   Gateway 5' entry clone

   Tags

   Expression

   Mutation

   mutated single nucleotide (T-->C, 13th nucleot…

   Promoter

   lymphocyte-specific protein tyrosine kinase (lck)…

   Available since

   Aug. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV-T7-SpCas9-BPNLS(SV40)-3xFLAG-P2A-mScarlet (MNW001)

   Plasmid

   #174091

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpCas9 with a C-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized SpCas9 with BPNLS-3xFLAG-P2A-mScarlet
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   pCMV and T7

   Available since

   Sept. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBAD_IPF2.0

   Plasmid

   #163124

   Purpose

   A plasmid with IFP2.0 coding sequence coded into it. Expresses IFP2.0 with N-term His-tag.

   Depositor

   Julie Biteen

   Article

   Chia et al Curr Res Microb Sci. 2020 Sep;1:1-6. doi

   Insert

   IFP2.0
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTRE-T2-IRES-His6-Ubiquitin

   Plasmid

   #107107

   Purpose

   Designed to co-express target gene upstream of the IRES element at the multiple cloning site with downstream His6-tagged ubiquitin expressed after IRES.

   Depositor

   Michael E. Wright

   Insert

   human ubiquitin UBB (UBB Human)
   

   Use

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   Tet

   Available since

   Aug. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. saposin B^W

    Plasmid

    #161759

    Purpose

    Saposin B (Homo Sapien) containing a single mutation, arginine 38 to tryptophan

    Depositor

    Sam Gellman

    Article

    Kurgan et al Biochemistry. 2021 Apr 13;60(14):1108-11

    Insert

    saposin B R38_W
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    changed Arginine 38 to Tryptophan

    Promoter

    T7

    Available since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. psd44-G13QL

    Plasmid

    #46828

    Purpose

    expression of mutant G protein alpha 13 in mammalian cells

    Depositor

    Agnese Mariotti

    Insert

    GNA13 (GNA13 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Q226L (The mutation reduces GTPase activity resul…

    Promoter

    PGK promoter

    Available since

    July 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pScDD2_ERdd

    Plasmid

    #109047

    Purpose

    destabilizing domains for yeast (S. cerevisiae) derived from the human estrogen receptor ligand binding domain. This ERdd can be stabilized with 10 uM 4-hydroxytamoxifen (4-OHT, OHTAM).

    Depositor

    Thomas Wandless

    Insert

    ER50 (ESR1 H. sapiens gene with yeast enhanced codon)
    

    Use

    Tags

    yeast enhanced GFP with 8 amino acids TSGRGEGQ li…

    Expression

    Yeast

    Mutation

    3 mutations : E380G, R434G, N532S

    Promoter

    SOD1

    Available since

    April 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAG426-GAL-Alpha-synuclein-A53T

    Plasmid

    #181722

    Purpose

    Galactose inducible expression of Alpha-synuclein-A53T

    Depositor

    Alejandro Chavez

    Insert

    Alpha-synuclein (SNCA Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    A53T

    Promoter

    Available since

    July 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. ACE2 g1 + Cas9

    Plasmid

    #153011

    Purpose

    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2

    Depositor

    Jason Sheltzer

    Insert

    Cas9 + ACE2 gRNA (ACE2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    TagBFP2

    Expression

    Mutation

    Promoter

    Available since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-UbC-eGFP-F

    Plasmid

    #71545

    Purpose

    Expresses a membrane bound eGFP, under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV or β-actin-CMV promoters.

    Depositor

    Pantelis Tsoulfas

    Insert

    eGFP-F
    

    Use

    AAV

    Tags

    contains 20- amino-acid farnesylation signal from…

    Expression

    Mammalian

    Mutation

    Promoter

    UbC

    Available since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLKO.1-blast shKGA

    Plasmid

    #110420

    Purpose

    shKGA, silence glutaminase KGA isoform, blasticidin selection.

    Depositor

    Sandra Martha Gomes Dias

    Insert

    GLS glutaminase (GLS Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 (RNA Pol III)

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. Lamin A (R377H)-mRFP

    Plasmid

    #124270

    Purpose

    Lamin A mutant (R377H), C-terminally tagged with mRFP. Mutation associated with EDMD myopathy.

    Depositor

    Eric Schirmer

    Insert

    LMNA (R377H) (LMNA Human)
    

    Use

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    LMNA (R377H)

    Promoter

    Available since

    July 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. Hs.KRAS4b S17N

    Plasmid

    #83156

    Purpose

    Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), S17N mutation

    Depositor

    Dominic Esposito

    Insert

    KRAS (KRAS Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    S17N

    Promoter

    Available since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pALS3-Ma-SUMO-sfGFP-WT

    Plasmid

    #212122

    Purpose

    Fluorescence control plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses SUMO-sfGFP-WT and M. alvus Pyl-tRNA(6). p15a origin. Tetracycline resistance.

    Depositor

    Ryan Mehl

    Inserts

    SUMO-sfGFP-WT
    M. alvus Pyl-tRNA(6)
    

    Use

    Tags

    His6 and SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    araC and lpp

    Available since

    Sept. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBACgus N-His/GST/LRRK2 1867-2186

    Plasmid

    #40403

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Baculovirus

    Tags

    GST-Tev and HisTev

    Expression

    Mutation

    WT aa1867-2186

    Promoter

    polH

    Available since

    Oct. 1, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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