162,717 results

1. CRE recombinase

   Plasmid

   #62730

   Purpose

   Expresses Cre recombinase protein in bacterial cells

   Depositor

   Niels Geijsen

   Article

   D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1

   Insert

   Cre recombinase
   

   Use

   Cre/Lox

   Tags

   His tag and NLS

   Expression

   Bacterial

   Mutation

   Codon optimazed for e.coli

   Promoter

   T7

   Available since

   April 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTipi2.1_Anti-Strep [IPI-C23.21] Mouse/Rabbit HC

   Plasmid

   #221355

   Purpose

   Mammalian expression of Anti-Strep [IPI-C23.21] Mouse/Rabbit HC; to be used with Anti-Strep [IPI-C23.21] light chain (Plasmid 221356) to make the antibody.

   Depositor

   Institute for Protein Innovation (IPI)

   Insert

   Anti-Strep [IPI-C23.21] Mouse/Rabbit HC
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pTipi2.1_Anti-Strep [IPI-C23.21] Mouse/Rabbit LC kappa

   Plasmid

   #221356

   Purpose

   Mammalian expression of Anti-Strep [IPI-C23.21] Mouse/Rabbit LC kappa; to be used with Anti-Strep [IPI-C23.21] heavy chain (Plasmid 221355) to make the antibody.

   Depositor

   Institute for Protein Innovation (IPI)

   Insert

   Anti-Strep [IPI-C23.21] Mouse/Rabbit LC kappa
   

   Use

   Affinity Reagent/ Antibody

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 1, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pLVX-UbC-rtTA-Ngn2:2A:Ascl1

   Plasmid

   #127289

   Purpose

   all-in-one inducible LV expression construct for Ngn2:2A:Ascl1 (aka UNA; for direct neuronal conversion)

   Depositor

   Fred Gage

   Article

   Herdy et al Elife. 2019 May 17;8. pii: 41356. doi: 1

   Inserts

   Ngn2:2A:Ascl1 (ASCL1, NEUROG2 Human)
   Ngn2:2A:Ascl1 (ASCL1, NEUROG2 Human)
   

   Use

   Lentiviral

   Tags

   Ngn2 and Ascl1 linked via 2A

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. FLAG-SKP2

   Plasmid

   #215092

   Purpose

   Expresses FLAG-SKP2 in mammalian cells.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   SKP2 (SKP2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pWZL-Blast FAPwt

   Plasmid

   #210643

   Purpose

   Expresses the FAP (Fibroblast Activation Protein) protein.

   Depositor

   Jing Yang

   Article

   Bukhari et al Cell Rep. 2023 Oct 31;42(10):113302. doi

   Insert

   Fibroblast Activation Protein Alpha (FAP Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   none

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. FLT3-ITD-MSCV-IRES-GFP

   Plasmid

   #184779

   Purpose

   Expresses FLT3-ITD (internal tandem duplication) constitutively active mutant in mammalian cells

   Depositor

   Timothy Ley

   Insert

   FLT3 (FLT3 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Activating internal tandem duplication (ITD) adde…

   Promoter

   MSCV

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Nuclear PancACe

   Plasmid

   #215706

   Purpose

   Expresses acetyl-CoA biosensor in the nucleus

   Depositor

   Katharine Diehl

   Article

   Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

   Insert

   PancACe
   

   Use

   Lentiviral

   Tags

   FLAG, HA, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET-pAG-Hia5

   Plasmid

   #174373

   Purpose

   Plasmid for IPTG-inducible expression and purification of protein A/G-Hia5-6xHis (pAG-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)

   Depositor

   Aaron Straight

   Article

   Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi

   Insert

   protein AG - hia5
   

   Use

   Tags

   6xHis and Protein A/G

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. LAMP1-mGFP

    Plasmid

    #34831

    Depositor

    Esteban Dell'Angelica

    Article

    Falcon-Perez et al J Cell Sci. 2005 Nov 15;118(Pt 22):5243-

    Insert

    LAMP1 (LAMP1 Human)
    

    Use

    Tags

    GSTGSTGSTGA linker, Kozak, and mGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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