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pDN-G1Tt

Plasmid

#44509

Depositor

Gabor Balazsi

Article

Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

Inserts

PGal1-D12 promoter (GAL1 Budding Yeast, Synthetic)
tetR

Use

Synthetic Biology; Expression regulator/reporter

Expression

Yeast

Mutation

Two tandem tet operators (2XtetO2) downstream of …

Promoter

PGal1-D12 promoter

Available Since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX-2TK cyclin T1 (1-250) (P#467)

Plasmid

#14606

Depositor

Matija Peterlin

Article

Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 9

Insert

cyclin T1 (1-250) (CCNT1 Human)

Tags

GST

Expression

Bacterial

Mutation

amino acids 1-250

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

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pETDuet-1-6xHis-TEV-Nsp8

Plasmid

#201022

Purpose

Bacterial expression of codon optimized SARS-CoV-2 nsp8 tagged with an N-terminus His-tag followed by a TEV protease cleavage site.

Depositor

Sharon Rozovsky

Article

Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

Insert

non-structural protein 8 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)

Tags

6xHis-TEV

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-AIRAPL-Strep-HA

Plasmid

#113489

Purpose

Expression of human AIRAPL with C-terminal strep-HA tag

Depositor

Hemmo Meyer

Article

Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

Insert

AIRAPL (ZFAND2B Human)

Tags

2xstrep-HA

Expression

Mammalian

Mutation

wildtype without stop codon

Promoter

CMV

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-MKL1 D301-380

Plasmid

#19852

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

Insert

MKL1 (MRTFA Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

deleted amino acids 301 to 380

Available Since

Dec. 16, 2008

Availability

Academic Institutions and Nonprofits only
p3xFLAG-CMV-RNF8-N-terminus(1-140)

Plasmid

#64675

Purpose

expresses only N terminus of RNF8, amino acids 1 to 140

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

Ring finger protein 8 (RNF8 Human)

Tags

FLAG

Expression

Mammalian

Mutation

only N terminus amino acids 1-140

Promoter

CMV

Available Since

May 20, 2015

Availability

Academic Institutions and Nonprofits only
pDEST27-GST-ATXN1 FL(2Q)

Plasmid

#21752

Depositor

Huda Zoghbi

Article

Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

Insert

Ataxin-1 (ATXN1 Human)

Tags

GST

Expression

Mammalian

Mutation

insert contains 2Q (2 glutamines) and there isno …

Promoter

CMV

Available Since

April 17, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3 Flag MFF iso1 WT

Plasmid

#74388

Purpose

Flag tagged human MFF isoform 1

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

Flag-MFF (MFF Human)

Tags

Flag

Expression

Mammalian

Mutation

delta exon 1 (1-26)

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only
pTRIP-SFFV-GFP-RELA K5R

Plasmid

#196631

Purpose

Express GFP-RELA K5R

Depositor

Nicolas Manel

Article

Jeremiah et al J Exp Med. 2023 May 1;220(5):e20220666.

Insert

GFP-RELA K5R (RELA Human)

Use

Lentiviral

Tags

GFP

Mutation

K122R, K123R, K310R, K314R, K315R

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-WRAP53beta-DC-mutant-G435R

Plasmid

#64684

Purpose

expresses single mutated WRAP53beta at G435R, found in Dyskeratosis congenita patients

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

WD40-encoding RNA antisense to P53 (WRAP53 Human)

Tags

GFP

Expression

Mammalian

Mutation

mutation at G435R

Promoter

CMV

Available Since

May 21, 2015

Availability

Academic Institutions and Nonprofits only
pEGFP-WRAP53beta-DC-mutant-R398W

Plasmid

#64683

Purpose

expresses single mutated WRAP53beta at R398W, found in Dyskeratosis congenita patients

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

WD40-encoding RNA antisense to P53 (WRAP53 Human)

Tags

GFP

Expression

Mammalian

Mutation

mutation at R398W

Promoter

CMV

Available Since

May 21, 2015

Availability

Academic Institutions and Nonprofits only
pEE038

Plasmid

#176795

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

407

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEE048

Plasmid

#176805

Purpose

Encodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expression

Depositor

Gregg Beckham

Article

Erickson et al Nat Commun. 2022 Dec 21;13(1):7850. doi:

Insert

504

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pGL2 p15 4xSBR2 Mut 3' box

Plasmid

#15708

Depositor

Joan Massague

Article

Gomis et al Cancer Cell. 2006 Sep . 10(3):203-14.

Insert

p15 4xSBR2 (CDKN2B Human)

Use

Luciferase

Expression

Mammalian

Mutation

Mutant 3' box. The 3' box contains the …

Available Since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA-p160(400-580)

Plasmid

#45

Depositor

Bruce Spiegelman

Article

Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

Insert

p160 myb binding protein (Mybbp1a Mouse)

Tags

myc

Expression

Mammalian

Mutation

expresses only aa400-580

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA-p160(200-400)

Plasmid

#44

Depositor

Bruce Spiegelman

Article

Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

Insert

p160 myb binding protein (Mybbp1a Mouse)

Tags

myc

Expression

Mammalian

Mutation

Expresses only aa200-400

Available Since

July 21, 2005

Availability

Academic Institutions and Nonprofits only
pcDNA3 Flag MFF iso4 WT

Plasmid

#74390

Purpose

Flag tagged human MFF isoform 4

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

Flag-MFF (MFF Human)

Tags

Flag

Expression

Mammalian

Available Since

April 13, 2016

Availability

Academic Institutions and Nonprofits only
pDual-eGFP(Stop66)

Plasmid

#63218

Purpose

Expression of a non-sense mutant version of eGFP (dark) in bacteria and in mammalian cells. Could be used as a reporter for gene targeting and recombineering in mammalian cells.

Depositor

Richard S Myers

Article

Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1

Insert

His-T7-eGFP(Stop66)

Use

Lentiviral

Tags

T7 and his6

Expression

Bacterial and Mammalian

Mutation

Changed tyrosine at position 66 with respect to t…

Promoter

CMV-EF1α hybrid (CEF)

Available Since

April 27, 2015

Availability

Academic Institutions and Nonprofits only
pGL3b(1454/3172)P2P-wt

Plasmid

#21404

Depositor

Eric Metzen

Article

Metzen et al Biochem J. 2005 May 1. 387(Pt 3):711-7.

Insert

EGLN1 (EGLN1 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Sept. 9, 2009

Availability

Academic Institutions and Nonprofits only
pCin4-3xFLAG-MKL1 D444-500

Plasmid

#19839

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

Insert

MKL1 D444-500 (MRTFA Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

deleted amino acids 444-500

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only