We narrowed to 14,023 results for: egf

1. pBa-eGFP-flag-BicD2 594-FKBP

   Plasmid

   #63569

   Purpose

   Bicaudal D protein aa1-594 (excluding start Met) tagged with N-terminal eGFP and flag and a C-terminal FKBP

   Depositor

   Gary Banker, Marvin Bentley

   Article

   Bentley et al J Cell Biol. 2015 Feb 2;208(3):273-281.

   Insert

   Bicaudal D protein (Bicd2 Mouse)
   

   Use

   Tags

   FKBP, eGFP, and flag

   Expression

   Mammalian

   Mutation

   no start methionine, aa1-594

   Promoter

   chicken Beta Actin

   Available Since

   April 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGADT7-ADH700-yeCherry-YAP1-yeGFP-DHFR

   Plasmid

   #24549

   Depositor

   Thomas Wandless

   Article

   Edwards et al Yeast. 2010 Apr;27(4):229-36. doi: 10.10

   Insert

   pADH1 yeCherry::YAP1 yeGFP-DHFR (YAP1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   1. Truncated version of ADH700 promoter (SbfI/Spe…

   Promoter

   Available Since

   May 3, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDisplay-GRAB-rDA2h-IRES-EGFP-CAAX

   Plasmid

   #208695

   Purpose

   Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA2h and a membrane-localized EGFP in mammalian cells

   Depositor

   Yulong Li

   Article

   Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

   Insert

   GPCR activation based dopamine (DA) sensor GRAB_rDA2h
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. JDW 742 (pDONR221-EGFP-KRAS4B-G12V)

   Plasmid

   #156403

   Purpose

   Gateway middle entry clone encoding EGFP-fused to human KRAS4B with G12V mutation, includes a stop codon

   Depositor

   Joshua Wythe

   Article

   Fish et al Circ Res. 2020 Jun 17. doi: 10.1161/CIRC

   Insert

   human mutant KRAS4B-G12V (KRAS Human)
   

   Use

   Gateway cloning

   Tags

   EGFP

   Expression

   Mutation

   mutant KRAS4B-G12V

   Promoter

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYR69 (X-4:4xUAS-SSA1p-365:yEGFP3-CYC1t)

   Plasmid

   #167355

   Purpose

   Integrative plasmid for promoter-reporter 4xUAS-SSA1p-365:yEGFP3-CYC1t

   Depositor

   Michael Jensen

   Article

   Romero-Suarez et al ACS Synth Biol. 2021 Feb 12. doi: 10.102

   Insert

   4xUAS-SSA1p-365:yEGFP3-CYC1t
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-EF1a-DIO-APEX.NES-P2A-EGFP

   Plasmid

   #182824

   Purpose

   Cre-dependent expression of cytosolic APEX

   Depositor

   Yevgenia Kozorovitskiy

   Article

   Dumrongprechachan et al Nat Commun. 2021 Aug 11;12(1):4855. doi:

   Insert

   APEX.NES-P2A-EGFP
   

   Use

   AAV

   Tags

   EGFP reporter (P2A)

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. EK0294 SFFV-mCherry-SG(s70587-W)SG-EGFP (FLP-IN)

   Plasmid

   #191141

   Purpose

   Positive control for Sensor 1 (EK0285) where the TAG stop codon is replaced with TGG tryptophan codon

   Depositor

   Xiaojing Gao

   Article

   Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

   Insert

   mCherry:s70587-W:EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   UAG>UGG (stop>Trp)

   Promoter

   SFFV

   Available Since

   Oct. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pEGFP-C1-GFP-SF3B6-Delete-NLS-FLAG

   Plasmid

   #86871

   Purpose

   To express SF3B6 delete NLS signal with a GFP and FLAG tags at N- and C-terminus, respectively.

   Depositor

   Yihong Ye

   Article

   Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

   Insert

   SF3B6 (SF3B6 Human)
   

   Use

   Tags

   EGFP and FLAG

   Expression

   Mammalian

   Mutation

   amino acids 7-29 deletion

   Promoter

   CMV

   Available Since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAY19 (OsTIR1(F74A) mAID-EGFP-NLS)

   Plasmid

   #140535

   Purpose

   OsTIR1(F74A)-P2A-mAID-EGFP-NLS TOL2

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   mAID-EGFP-NLS TOL2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. NSK024 SFFV-mCherry-SG(p53R248Q.s2)SG-EGFP (FLP-IN)

    Plasmid

    #191169

    Purpose

    Expression of a sensor for p53 mutant R248Q in mammalian cells; mCherry marker, EGFP output

    Depositor

    Xiaojing Gao

    Article

    Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

    Insert

    mCherry:p53(R248Q).s2:EGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. CAG-ABEmax-P2A-EGFP-ires-puro

    Plasmid

    #121170

    Purpose

    CAG promoter driven expression of ABEmax base editor, EGFP and Puromycin resistance.

    Depositor

    Timo Otonkoski

    Insert

    ABEmax-P2A-EGFP-ires-puro
    

    Use

    CRISPR

    Tags

    P2A-EGFP-ires-puro

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pD649-HAsp-EGFL7-Fc(DAPA)-AviTag-6xHis

    Plasmid

    #156702

    Purpose

    Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    EGFL7 (EGFL7 Human)
    

    Use

    Tags

    Fc(DAPA)-AviTag-6xHis

    Expression

    Mammalian

    Mutation

    Fc region of human IgG contains D265A and P329A m…

    Promoter

    CMV/SP6

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSbi-pur-KSR1-CA3-EGFP (C-KSR)

    Plasmid

    #217768

    Purpose

    Fluorescent reporter for ceramide (for stable mammalian expression)

    Depositor

    Paula Nunes-Hasler

    Article

    Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

    Insert

    Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
    

    Use

    Sleeping beauty transposase-compatible genome ins…

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    CA3 domain aa 317-400 plus vector-based linker LE…

    Promoter

    EF-1alpha promoter

    Available Since

    June 10, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pAAV-CAG-EGFP barcode-5-SV40 polyA

    Plasmid

    #190869

    Purpose

    For barcoded retrograde labeling

    Depositor

    Yuejun Chen

    Article

    Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

    Insert

    EGFP-barcode5
    

    Use

    AAV

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available Since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. phage - ubc - nls - 2xmcp - egfp - BirA*

    Plasmid

    #131132

    Purpose

    BirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.

    Depositor

    Ralf Jansen

    Article

    Mukherjee et al Proc Natl Acad Sci U S A. 2019 Jun 25;11

    Insert

    NLS-HA-2xMCP-EGFP-BirA*
    

    Use

    Lentiviral

    Tags

    NLS, HA

    Expression

    Mammalian

    Mutation

    BirA* = BIrA >R118G

    Promoter

    human ubiquitin C (UBC)

    Available Since

    Aug. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-TRE-Gluc-RMA-IRES-EGFP

    Plasmid

    #189628

    Purpose

    Expresses Gluc-RMA and EGFP driven by the Tet Response Element (TRE). Plasmid 2 for recording Fos expression.

    Depositor

    Jerzy Szablowski

    Article

    Lee et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Inserts

    Gaussia luciferase fused to Fc
    IRES-EGFP
    

    Use

    AAV and Luciferase

    Tags

    Gluc and IgG1 Fc

    Expression

    Mammalian

    Mutation

    Promoter

    IRES and TRE

    Available Since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKS031 - pCAGGS-3XFLAG-BORIS-eGFP-bpA

    Plasmid

    #156443

    Purpose

    For transient expression of mouse BORIS (CTCFL) tagged with eGFP

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    mouse BORIS (Ctcfl Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available Since

    Sept. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV-CMV-EGFP-PolB-PAMmut-Hygro

    Plasmid

    #176086

    Purpose

    EGFP fused to the N-terminus of a POLB containing a mutation in the PAM site used by POLBKOg1 & a hygromycin resistance cassette

    Depositor

    Robert Sobol

    Article

    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

    Insert

    PolB (POLB Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Mutation in the PAM site used by PolBKOg1

    Promoter

    CMV

    Available Since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLVX-EF1α-ZC3H12D-D95N-IRES-EGFP

    Plasmid

    #153071

    Purpose

    Lentiviral vector expressing human ZC3H12D mutated (D95N)

    Depositor

    Silvia Monticelli

    Article

    Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

    Insert

    Mutated ZC3H12D (D95N) (ZC3H12D Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Changed D95 to N, known to abrogate the RNase enz…

    Promoter

    EF1α

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pME-Lifeact-EGFP-3xHA (JDW 1322)

    Plasmid

    #224494

    Purpose

    Gateway compatible middle entry clone containing Lifeact-EGFP (EGFP F-actin reporter)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    Liefact-EGFP-3xHA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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