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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 146,921 results for: ins;

Showing: 6341 - 6360 of 146921 results

  2. 1a. pETM11-SUMO-SNCA-GFP

    Plasmid
    #107292
    Purpose
    Plasmid for the expression of the construct alpha-Synuclein-GFP, which contains N-terminal tags, which can be cleaved later: His-tag for purification and SUMO-tag for better solubility
    Depositor
    Dmytro Yushchenko
    Article
    Afitska et al Biophys J. 2017 Nov 21;113(10):2182-2191
    Insert
    SNCA-GFP (SNCA Human)
    Use
    Tags
    His-tag and SUMO-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    May 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    107292-plasmid-map-sequence-id-209472

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  3. AAV-PR rep/cap

    Plasmid
    #197565
    Purpose
    encodes AAV-PR capsid that transduces pericytes and smooth muscle cells in mice after systemic delivery
    Depositor
    Casey Maguire
    Article
    Ramirez et al Hum Gene Ther. 2023 Jun 27. doi: 10.1089
    Inserts
    AAV Rep genes
    AAV9 VP1 Cap gene with PR insert
    Use
    AAV
    Tags
    Expression
    Mutation
    contains 7mer insert PRPPSTH between amino acids …
    Promoter
    Available Since
    June 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    197565-plasmid-map-sequence-id-388046

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  6. pRRL.SIN.EF1A.CD19-FMC63.218.CAR-3G

    Plasmid
    #194458
    Purpose
    CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); CD28, 4-1BB & CD3ζ (signaling domains); anti-CD19 from FMC63 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.
    Depositor
    Ana Sofia Coroadinha
    Article
    Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:
    Insert
    Anti-CD19 CAR
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    194458-plasmid-map-sequence-id-384169

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  8. pEC2935 (p7INT-P23_mNeongreen~ssrA (LDD variant))

    Plasmid
    #218508
    Purpose
    integrative plasmid enabling constitutive expression of a destabilized mNeongreen reporter in Streptococcus pyogenes, targets the attP site of phage T12 in several S. pyogenes strains via an integrase
    Depositor
    Emmanuelle Charpentier
    Article
    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1
    Insert
    mNeongreen
    Use
    Tags
    modified ssrA degradation tag
    Expression
    Bacterial
    Mutation
    deletion of MCS containing Plac_lacZa, introducti…
    Promoter
    P23
    Available Since
    July 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    218508-plasmid-map-sequence-id-438746

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  9. pCAG-CBE4max-SpG-P2A-EGFP (RTW4552)

    Plasmid
    #139998
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized CBE4max SpCas9 variant named SpG with P2A-EGFP
    Use
    CRISPR
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…
    Promoter
    CAG
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139998-plasmid-map-sequence-id-394975

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  11. pNCST-AvicFP4

    Plasmid
    #129507
    Purpose
    Expresses AvicFP4 constitutively in E. coli (most strains)
    Depositor
    Nathan Shaner
    Article
    Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d
    Insert
    AvicFP4
    Use
    Tags
    Expression
    Bacterial
    Mutation
    One of two variants of AvicFP4 tested with essent…
    Promoter
    synthetic constitutive (stationary phase) promote…
    Available Since
    Feb. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    129507-plasmid-map-sequence-id-271859

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  12. pTarget

    Plasmid
    #214052
    Purpose
    Expresses target sequence for Haliangium type III CRISPR-Cas complex; identical to pNon-target (Addgene plasmid # 214053) but contains a protospacer.
    Depositor
    Raymond Staals
    Article
    Steens et al Science. 2024 Feb 2;383(6682):512-519. d
    Insert
    Target sequence
    Use
    Target rna plasmid
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    trc promoter
    Available Since
    June 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    214052-plasmid-map-sequence-id-428822

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  13. pHochTypeIII

    Plasmid
    #214039
    Purpose
    Bacterial expression plasmid for Haliangium ochraceum type III CRISPR-Cas complex; contains csb2, a minimal CRISPR array with a pTarget spacer, cmr1-6
    Depositor
    Raymond Staals
    Article
    Steens et al Science. 2024 Feb 2;383(6682):512-519. d
    Insert
    Cas10
    Use
    CRISPR
    Tags
    Expression
    Mutation
    WT
    Promoter
    T7 promoter
    Available Since
    June 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    214039-plasmid-map-sequence-id-424943

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  19. pRRL.SIN.EF1A.CD19-FMC63.218.CAR-2G

    Plasmid
    #194457
    Purpose
    CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ζ (signaling domains); anti-CD19 from FMC63 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.
    Depositor
    Ana Sofia Coroadinha
    Article
    Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:
    Insert
    Anti-CD19 CAR
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    March 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    194457-plasmid-map-sequence-id-384274

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Showing: 6341 - 6360 of 146921 results