We narrowed to 6,946 results for: crispr cas9 plasmids

1. px330-CD4

   Plasmid

   #136938

   Purpose

   NHEJ assay. sgRNA/Cas9 plasmid. Target DSB at human CD4; induce CD4+ deletion rearrangement by pairing w/ px330-GAPDH

   Depositor

   Jeremy Stark

   Article

   Bhargava et al J Biol Chem. 2019 Nov 21. pii: RA119.010

   Insert

   sgRNA targeting CD4 (CD4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJT131_GalL_cCDA1-NGBE3

   Plasmid

   #145095

   Purpose

   Expressing base editor cCDA1-NGBE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

   Insert

   cCDA1-NGBE3
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   spCas9(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1…

   Promoter

   GalL

   Available since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJT174_GalL_A3A-Y130F-BE3

   Plasmid

   #145123

   Purpose

   Expressing base editor A3A-Y130F-BE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

   Insert

   A3A(Y130F)-BE3
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   A3A(Y130F); spCas9(D10A)

   Promoter

   GalL

   Available since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJT173_GalL_A3A-R128A-BE3

   Plasmid

   #145122

   Purpose

   Expressing base editor A3A-R128A-BE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

   Insert

   A3A(R128A)-BE3
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   A3A(R128A); spCas9(D10A)

   Promoter

   GalL

   Available since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJT113_GalL_cCDA1-xBE3

   Plasmid

   #145079

   Purpose

   Expressing base editor cCDA1-xBE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

   Insert

   cCDA1-xBE3
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   spCas9(D10A, A262T, R324L, S409I, E480K, E543D, M…

   Promoter

   GalL

   Available since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRN3P_T3_ABE8e_IVT

   Plasmid

   #201676

   Purpose

   Plasmid to be used as DNA template for in vitro RNA transcription of the ABE8e base editor (A to G) by T3 RNA polymerase. The plasmid contains optimised 5'UTR and 3'UTR to improve protein expression.

   Depositor

   Timo Otonkoski

   Article

   Jalil et al Am J Hum Genet. 2024 Apr 4;111(4):714-72

   Insert

   ecTadA(8e)-nSpCas9
   

   Use

   CRISPR; Vector for in-vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   T3 promoter

   Available since

   April 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pV1524

   Plasmid

   #111431

   Purpose

   CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Mal2-FLP)

   Depositor

   Gerald Fink

   Article

   Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

   Insert

   CaCas9/sgRNA-BsmBI stuffer
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pV1393

   Plasmid

   #111430

   Purpose

   CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Sap2-FLP)

   Depositor

   Gerald Fink

   Article

   Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

   Insert

   CaCas9/sgRNA-BsmBI stuffer
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEG302_22aa_SunTag_MQ1(Q147L)_g4+g10+g18

   Plasmid

   #172318

   Purpose

   CRISPR dCas9 SunTag system to target a variant of bacterial DNA methyltransferase MQ1 to install CG specific methylation to the FWA locus with three guide RNAs

   Depositor

   Steven Jacobsen

   Article

   Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

   Insert

   g18_U6_g10_U6_g4_U6_NOS_MQ1(Q147L)_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYPQ166-SpRY

    Plasmid

    #161520

    Purpose

    Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zSpRYCas9-NLS without promoter

    Depositor

    Yiping Qi

    Article

    Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41

    Insert

    zSpRYCas9
    

    Use

    CRISPR; Gateway compatible zsprycas9 entry clone

    Tags

    3X FLAG, NLS and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. PLJR965_Rv3297

    Plasmid

    #241235

    Purpose

    CRISPRi gene silencing plasmid with sgRNA (Rv3297) dCas9 TetR and KanR L5 Int attP for M. tuberculosis

    Depositor

    Kyu Rhee

    Insert

    Rv3297 sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    Sept. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PLJR965_Rv2976c

    Plasmid

    #241234

    Purpose

    CRISPRi gene silencing plasmid with sgRNA (Rv2976c) dCas9 TetR and KanR L5 Int attP for M. tuberculosis

    Depositor

    Kyu Rhee

    Insert

    Rv2976c sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    Sept. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. PLJR965_Rv2464c

    Plasmid

    #241232

    Purpose

    CRISPRi gene silencing plasmid with sgRNA (Rv2464c) dCas9 TetR and KanR L5 Int attP for M. tuberculosis

    Depositor

    Kyu Rhee

    Insert

    Rv2464c sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    Sept. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PLJR965_Rv1638

    Plasmid

    #241231

    Purpose

    CRISPRi gene silencing plasmid with sgRNA (Rv1638) dCas9 TetR and KanR L5 Int attP for M. tuberculosis

    Depositor

    Kyu Rhee

    Insert

    Rv1638 sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    Sept. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PLJR965_Rv1259

    Plasmid

    #241228

    Purpose

    CRISPRi gene silencing plasmid with sgRNA (Rv1259) dCas9 TetR and KanR L5 Int attP for M. tuberculosis

    Depositor

    Kyu Rhee

    Insert

    Rv1259 sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    Sept. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PLJR965_Rv1020

    Plasmid

    #241227

    Purpose

    CRISPRi gene silencing plasmid with sgRNA (Rv1020) dCas9 TetR and KanR L5 Int attP for M. tuberculosis

    Depositor

    Kyu Rhee

    Insert

    Rv1020 sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    Sept. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PLJR965_Rv0949

    Plasmid

    #241226

    Purpose

    CRISPRi gene silencing plasmid with sgRNA (Rv0949) dCas9 TetR and KanR L5 Int attP for M. tuberculosis

    Depositor

    Kyu Rhee

    Insert

    Rv0949 sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    Sept. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PLJR965_Rv1633

    Plasmid

    #241230

    Purpose

    CRISPRi gene silencing plasmid with sgRNA (Rv1633) dCas9 TetR and KanR L5 Int attP for M. tuberculosis

    Depositor

    Kyu Rhee

    Insert

    Rv1633 sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    Aug. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PLJR965_Rv3589

    Plasmid

    #241236

    Purpose

    CRISPRi gene silencing plasmid with sgRNA (Rv3589) dCas9 TetR and KanR L5 Int attP for M. tuberculosis

    Depositor

    Kyu Rhee

    Insert

    Rv3589 sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    Aug. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PLJR965_Rv2924c

    Plasmid

    #241233

    Purpose

    CRISPRi gene silencing plasmid with sgRNA (Rv2924c) dCas9 TetR and KanR L5 Int attP for M. tuberculosis

    Depositor

    Kyu Rhee

    Insert

    Rv2924c sgRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    Available since

    Aug. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge