Addgene

We narrowed to 15,745 results for: form

Showing: 931 - 960 of 15745 results

Results per page:

Sort results by:

pMSCV-GSDMD_NoCys-Myc

Plasmid

#201169

Purpose

Expression of GSDMD gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

Insert

Gasdermin D (Gsdmd Mouse)

Use

Retroviral

Tags

Myc

Expression

Mutation

C39A, C57A, C77A, C122A, C265A, C192A

Promoter

MESV

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-GSDMD_C192A-Myc

Plasmid

#201168

Purpose

Expression of GSDMD gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

Insert

Gasdermin D (Gsdmd Mouse)

Use

Retroviral

Tags

Myc

Expression

Mutation

C192A

Promoter

MESV

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pST_DHN_AtCor47 (pBS0784)

Plasmid

#185254

Purpose

For the mammalian expression of the Arabidopsis protein DHN_AtCor47. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

DHN_AtCor47

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMM2-rPMCA3b

Plasmid

#47763

Purpose

mammalian expression of rat PMCA3b

Depositor

Emanuel Strehler, John Penniston

Article

Enyedi et al J Biol Chem. 1997 Oct 31;272(44):27525-8

Insert

PMCA3b (Atp2b3 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Sept. 3, 2013

Availability

Academic Institutions and Nonprofits only
pFastBac1-OSF-TRIM5alpha(human)(R332P)

Plasmid

#79037

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5alpha(human R332P) (TRIM5 Human)

Use

Tags

OneSTrEP-FLAG

Expression

Insect

Mutation

Changed Arg 332 to Pro

Promoter

polyhedrin

Available since

March 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEYFP-N1-hRIP3-FL V460P

Plasmid

#61379

Purpose

Expresses Human full length RIPK3 with V460P mutation in the mammalian cells

Depositor

Francis Chan

Article

Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

Insert

Full length human RIP3 with V460Pmutation (RIPK3 Human)

Use

Tags

EYFP

Expression

Mammalian

Mutation

changed V460 to P

Promoter

Available since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only
pEYFP-N1-hRIP3-FL V458P

Plasmid

#61377

Purpose

Expresses Human full length RIPK3 with V458P mutation in the mammalian cells

Depositor

Francis Chan

Article

Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

Insert

Full length human RIP3 with V458Pmutation (RIPK3 Human)

Use

Tags

EYFP

Expression

Mammalian

Mutation

changed V458 to P

Promoter

Available since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only
pFastBac1-OSF-TRIM5alpha(African green monkey pygerythrus)

Plasmid

#79031

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5alpha(African green monkey pygerythrus)

Use

Tags

OneSTrEP-FLAG

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

March 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5 FRT TO myc Separase delta 55/S1126A

Plasmid

#59825

Purpose

Allows the integration of myc Separase delta 55/S1126A in the genome and Tet-inducible expression.

Depositor

Stephen Taylor

Article

Holland et al J Biol Chem. 2007 Aug 24;282(34):24623-3

Insert

Separase (ESPL1 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

delta 55/S1126A

Promoter

hybrid human cytomegalovirus (CMV)/TetO2 promoter

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
CSII-IDR2-AGMpygTRIM5-FOS

Plasmid

#79064

Purpose

Express African green monkey pygerythrus TRIM5alpha in mammalan cells

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

TRIM5alpha(African green monkey pygerythrus)-FOS

Use

Tags

OneSTrEP-FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac1-OSF-TRIMCyp (owl monkey)(K283D, Q287D)

Plasmid

#79036

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5Cyp (owl monkey) (K283D, Q287D)

Use

Tags

OneSTrEP-FLAG

Expression

Insect

Mutation

Changed Lys 283 to Asp and Gln 287 to Asp

Promoter

polyhedrin

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEYFP-N1-hRIP3-FL G457D

Plasmid

#61367

Purpose

Expresses Human full length RIPK3 with G457Dmutation in the mammalian cells

Depositor

Francis Chan

Article

Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

Insert

Full length human RIP3 with G457D mutation (RIPK3 Human)

Use

Tags

EYFP

Expression

Mammalian

Mutation

changed G457 to D

Promoter

Available since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only
pEYFP-N1-hRIP3-FL N464D

Plasmid

#61369

Purpose

Expresses Human full length RIPK3 with N464D mutation in the mammalian cells

Depositor

Francis Chan

Article

Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

Insert

Full length human RIP3 with N464D mutation (RIPK3 Human)

Use

Tags

EYFP

Expression

Mammalian

Mutation

changed N464to D

Promoter

Available since

Jan. 15, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-CHRFAM7A-YFP

Plasmid

#62634

Purpose

mammalian expression of human duplicated Alpha7 (CHRFAM7A) with YFP fused into M3-M4 loop

Depositor

Henry Lester

Article

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

CHRFAM7A (CHRFAM7A Human)

Use

Tags

Expression

Mammalian

Mutation

YFP inserted in M3-M4 loop

Promoter

CMV

Available since

March 10, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pMRX-IBU-pSu9-HaloTag7-SNAP

Plasmid

#217319

Purpose

Expresses the presequence of Neurospora crassa Fo-ATPase subunit 9 (residues 1–69) with HaloTag and SNAP tag for measuring mitophagy activity by Halo processing assay.

Depositor

Noboru Mizushima

Article

Yang et al EMBO J. 2024 Nov;43(22):5613-5634. doi:

Insert

Neurospora crassa Fo-ATPase subunit 9 (residues 1–69) with HaloTag and SNAP tag

Use

Retroviral

Tags

HaloTag and SNAP

Expression

Mutation

Promoter

CMV

Available since

Nov. 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMx/ATP9A(E195Q)-HA

Plasmid

#209256

Purpose

Mammalian expression of ATP9A mutant E195Q

Depositor

Hye-Won Shin

Article

Yagi et al Life Sci Alliance. 2025 Apr 15;8(7):e202

Insert

ATP9A (ATP9A Human)

Use

Retroviral

Tags

HA

Expression

Mutation

E195Q

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMx/ATP9B(E272Q)-HA

Plasmid

#209257

Purpose

Mammalian expression of ATP9B mutant E272Q

Depositor

Hye-Won Shin

Article

Yagi et al Life Sci Alliance. 2025 Apr 15;8(7):e202

Insert

ATP9B (ATP9B Human)

Use

Retroviral

Tags

HA

Expression

Mutation

E272Q

Promoter

Available since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHis-WzzE

Plasmid

#196661

Purpose

Expression of N-terminally His6-tagged WzzE from Pectobacterium atrosepticum in E.coli

Depositor

James Naismith

Article

Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

Insert

WzzE

Use

Tags

TEV-cleavable His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pGST-WzzE

Plasmid

#196662

Purpose

Expression of N-terminally GST-tagged WzzE from Pectobacterium atrosepticum in E.coli

Depositor

James Naismith

Article

Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

Insert

WzzE

Use

Tags

TEV-cleavable GST

Expression

Bacterial

Mutation

Valine insertion at position 2.

Promoter

T7

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pHis-mCherry-WzzE

Plasmid

#196663

Purpose

Expression of N-terminally His6-mCherry-tagged WzzE from Pectobacterium atrosepticum in E.coli

Depositor

James Naismith

Article

Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

Insert

WzzE

Use

Tags

His and mCherry

Expression

Bacterial

Mutation

Valine insertion at position 2 of WzzE.

Promoter

T7

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPglL-strep-mCherry

Plasmid

#196664

Purpose

Expression of C-terminally distrep tag II-mCherry-tagged PglL from Neisseria meningitidis in E.coli

Depositor

James Naismith

Article

Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

Insert

PglL

Use

Tags

TEV-cleavable distrep tag II and mCherry

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pPglL-strep-mClover3

Plasmid

#196665

Purpose

Expression of C-terminally distrep tag II-mClover3-tagged PglL from Neisseria meningitidis in E.coli

Depositor

James Naismith

Article

Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

Insert

PglL

Use

Tags

TEV-cleavable di-strep tag II and mClover3

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pWzyE-GST-distrepII

Plasmid

#196666

Purpose

Expression of C-terminally GST-distrep tag II-tagged WzyE from Pectobacterium atrosepticum in E.coli

Depositor

James Naismith

Article

Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

Insert

WzyE

Use

Tags

GST and TEV-cleavable di-strep tag II

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pWzyE-strep-mClover3

Plasmid

#196668

Purpose

Expression of C-terminally distrep tag II-mClover3-tagged WzyE from Pectobacterium atrosepticum in E.coli

Depositor

James Naismith

Article

Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

Insert

WzyE

Use

Tags

TEV-cleavable di-strep tag II and mClover3

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-GSDMD_C39A-Myc

Plasmid

#201167

Purpose

Expression of GSDMD gene in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Devant et al Cell Rep. 2023 Jan 31;42(1):112008. doi:

Insert

Gasdermin D (Gsdmd Mouse)

Use

Retroviral

Tags

Myc

Expression

Mutation

C39A

Promoter

MESV

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
p426-Cup1p-FusionRed

Plasmid

#188392

Purpose

Cu2+-dependent expression of red fluorescent protein in yeast cells

Depositor

Michihiko Kataoka

Article

Utsumi et al PLoS One. 2023 Apr 13;18(4):e0283002. do

Insert

FusionRed

Use

Tags

Expression

Yeast

Mutation

Promoter

CUP1 promoter

Available since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-EGFP-C1-HsNot1iso1_4-302_S

Plasmid

#147536

Purpose

Mammalian Expression of HsNot1iso1_4-302

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

HsNot1iso1_4-302 (CNOT1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-EGFP-C1-HsNot1iso1_1356-2370_S

Plasmid

#147539

Purpose

Mammalian Expression of HsNot1iso1_1356-2370

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

HsNot1iso1_1356-2370 (CNOT1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmNot1_1147-1717-K1277A_R

Plasmid

#147431

Purpose

Insect Expression of DmNot1_1147-1717-K1277A

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot1_1147-1717-K1277A (Not1 Fly)

Use

Tags

Expression

Insect

Mutation

2 non silent mutations compared to the sequence g…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmNot1_1149-2505-dsRNAres_R

Plasmid

#147432

Purpose

Insect Expression of DmNot1_1149-2505-dsRNAres

Depositor

Elisa Izaurralde

Article

Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

Insert

DmNot1_1149-2505-dsRNAres (Not1 Fly)

Use

Tags

Expression

Insect

Mutation

6 silent mutations compared to the sequence given…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge