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Addgene

We narrowed to 18,017 results for: Gateway

Showing: 1241 - 1280 of 18017 results
  1. pENT-L3-pFOGl-L5

    Plasmid
    #186364
    Purpose
    Entry clone with ORF encoding Ci-FOG cis-regulatory region flanked by Gateway recombination sequences
    Depositor
    Insert
    Friend Of Gata regulatory sequence
    Use
    Protein expression
    Tags
    Expression
    Mutation
    Promoter
    T7 promoter
    Available since
    Oct. 19, 2022
    Availability
    Academic Institutions and Nonprofits only
  2. PDEST-pSP172BSSPE-pFOGc::RfA-ECFP

    Plasmid
    #186403
    Purpose
    Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter ECFP under control of the pFOG ectodermal regulatory sequence.
    Depositor
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
  3. pDEST-pSP172BSSPE-Swa1::RfA-ECFP

    Plasmid
    #186402
    Purpose
    Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter eCFP under control of a reg. sequence in the Swa1 restriction site.
    Depositor
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
  4. pDEST-pSP172BSSPE-pFOGc::RfA-venus

    Plasmid
    #186401
    Purpose
    Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter Venus under control of the pFOG ectodermal regulatory sequence.
    Depositor
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
  5. pDEST-pSP172BSSPE-pFOGc::venus-RfA

    Plasmid
    #186400
    Purpose
    Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter Venus and a gene of interest under control of the pFOG ectodermal regulatory sequence.
    Depositor
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
  6. pDEST-pSP172BSSPE-Swa1::venus-RfA

    Plasmid
    #186398
    Purpose
    Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter Venus and a gene of interest under control of a reg. sequence in the Swa1 restriction site.
    Depositor
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 29, 2022
    Availability
    Academic Institutions and Nonprofits only
  7. pENT-L3-a-element-L4

    Plasmid
    #186366
    Purpose
    Entry clone with ORF encoding Otx cis-regulatory region flanked by Gateway recombination sequences
    Depositor
    Insert
    a-element early Otx neural enhancer
    Use
    Protein expression
    Tags
    Expression
    Mutation
    Promoter
    T7 promoter
    Available since
    Sept. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
  8. pDEST-pSP172BSSPE-Swa1::RfA-HA

    Plasmid
    #186405
    Purpose
    Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter HA tag under control of a reg. sequence in the Swa1 restriction site.
    Depositor
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
  9. PDEST-pSP172BSSPE-pFOGc::RfA-HA

    Plasmid
    #186406
    Purpose
    Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter HA tag under control of the pFOG ectodermal regulatory sequence.
    Depositor
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 9, 2022
    Availability
    Academic Institutions and Nonprofits only
  10. pMpGWB418

    Plasmid
    #68683
    Purpose
    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Modified EAR motif plant-specific repression doma…
    Expression
    Plant
    Mutation
    Promoter
    MpEF1alpha promoter
    Available since
    June 24, 2016
    Availability
    Academic Institutions and Nonprofits only
  11. p663-UBC-V5-TurboID-V5-STOP_IDG-K

    Plasmid
    #158159
    Purpose
    Gateway destination clone of TurboID-V5 (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Insert
    V5-TurboID-V5
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Aug. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
  12. pHAGE-CMV-Flag-PKMYT1_IDG-K

    Plasmid
    #135277
    Purpose
    Gateway destination clone of PKMYT1 (human) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomics
    Depositor
    Insert
    Flag-PKMYT1 (PKMYT1 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
  13. p663-UBC-V5-miniTurbo-V5-STOP_IDG-K

    Plasmid
    #158158
    Purpose
    Gateway destination clone of miniTurbo-V5 (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Insert
    V5-miniTurbo-V5
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Oct. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
  14. p663-UBC-2xV5-TurboID-NES-STOP_IDG-K

    Plasmid
    #158162
    Purpose
    Gateway destination clone of TurboID-NES (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Insert
    2xV5-TurboID-NES
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Oct. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
  15. pDONR221-ACE2

    Plasmid
    #158438
    Purpose
    Gateway Cloning compatible entry vector for the human ACE2 gene.
    Insert
    ACE2 (ACE2 Human)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
  16. R777-E125 Hs.MAPK1

    Plasmid
    #70409
    Purpose
    Gateway ORF clone of human MAPK1 [NM_002745.4] with stop codon (for native or N-terminal fusions)
    Depositor
    Insert
    MAPK1 (MAPK1 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
  17. R777-E083 Hs.FGFR1

    Plasmid
    #70367
    Purpose
    Gateway ORF clone of human FGFR1 [NM_001174063.1] with stop codon (for native or N-terminal fusions)
    Depositor
    Insert
    FGFR1 (FGFR1 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
  18. pDONR221-DPP4

    Plasmid
    #158439
    Purpose
    Gateway Cloning compatible entry vector for the human DPP4 gene.
    Insert
    DPP4 (DPP4 Human)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
  19. p663-UBC-2xV5-miniTurbo-NES-STOP_IDG-K

    Plasmid
    #158161
    Purpose
    Gateway destination clone of miniTurbo-NES (as control) tagged with N-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Insert
    2xV5-miniTurbo-NES
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Aug. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
  20. R777-E101 Hs.HRAS

    Plasmid
    #70385
    Purpose
    Gateway ORF clone of human HRAS [NM_005343.2] with stop codon (for native or N-terminal fusions)
    Depositor
    Insert
    HRAS (HRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
  21. p667-UBC-PKMYT1-V5_IDG-K

    Plasmid
    #135248
    Purpose
    Gateway destination clone of PKMYT1 (human) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Insert
    PKMYT1-V5 (PKMYT1 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    Jan. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
  22. R777-E126 Hs.MAPK1-nostop

    Plasmid
    #70410
    Purpose
    Gateway ORF clone of human MAPK1 [NM_002745.4] without stop codon (for C-terminal fusions)
    Depositor
    Insert
    MAPK1 (MAPK1 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
  23. R777-E006 Hs.AKT3-nostop

    Plasmid
    #70290
    Purpose
    Gateway ORF clone of human AKT3 [NM_005465.4] without stop codon (for C-terminal fusions)
    Depositor
    Insert
    AKT 3 (AKT3 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
  24. R777-E102 Hs.HRAS-nostop

    Plasmid
    #70386
    Purpose
    Gateway ORF clone of human HRAS [NM_005343.2] without stop codon (for C-terminal fusions)
    Depositor
    Insert
    HRAS (HRAS Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
  25. 8522-E13

    Plasmid
    #234297
    Purpose
    Gateway ORF Entry clone of human SMAD3 with stop codon (for native or N-terminal fusions); T179V/S204A/S208A/S213A mutations (All four linker sites dead)
    Depositor
    Insert
    SMAD3 T179V/S204A/S208A/S213A (SMAD3 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    T179V/S204A/S208A/S213A; All four linker sites de…
    Promoter
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
  26. 9336-E23

    Plasmid
    #234306
    Purpose
    Gateway ORF Entry clone of human SMAD3 EPSM-CSD with stop codon (for native or N-terminal fusions); Linker sites dead, C-terminal constitutively active
    Depositor
    Insert
    SMAD3 EPSM-CSD (SMAD3 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    T179V/S204A/S208A/S213A/S423D/S425D; Linker sites…
    Promoter
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
  27. 9336-E21

    Plasmid
    #234304
    Purpose
    Gateway ORF Entry clone of human SMAD3 EPSM-SA with stop codon (for native or N-terminal fusions); Linker and C-terminal sites all dead
    Depositor
    Insert
    SMAD3 EPSM-SA (SMAD3 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    T179V/S204A/S208A/S213A/S422A/S423A/S425A; Linker…
    Promoter
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
  28. 9336-E26

    Plasmid
    #234309
    Purpose
    Gateway ORF Entry clone of human SMAD3 LSD-CSD with stop codon (for native or N-terminal fusions); Linker and C-terminal sites all constitutively active
    Depositor
    Insert
    SMAD3 LSD-CSD (SMAD3 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    T179D/S204D/S208D/S213D/S423D/S425D; Linker and C…
    Promoter
    Available since
    May 5, 2025
    Availability
    Academic Institutions and Nonprofits only
  29. pLX_TRC311

    Plasmid
    #113668
    Purpose
    Gateway destination vector for lentiviral expression
    Depositor
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Sept. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
  30. pLexAgtwy

    Plasmid
    #11345
    Depositor
    Insert
    gateway cassette
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    July 19, 2006
    Availability
    Academic Institutions and Nonprofits only
  31. pEF 3-YFP-2

    Plasmid
    #20106
    Depositor
    Insert
    YFP
    Use
    Synthetic Biology; Plant gateway vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 11, 2009
    Availability
    Academic Institutions and Nonprofits only
Showing: 1241 - 1280 of 18017 results