Skip to main content
Addgene
Showing: 1551 - 1600 of 9150 results

  1. pTJV1Sc-rpoB

    Plasmid
    #166978
    Purpose
    Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance
    Depositor
    Christopher Reisch
    Article
    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00
    Inserts
    Ec86 Retron (ECD_00831 )
    lambda bet
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    J23101 and VanCC
    Available since
    May 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    166978-plasmid-map-sequence-id-331328

    Enlarge

  4. pSS9

    Plasmid
    #71655
    Purpose
    Contains 600 bp homology arms to SS9 for one-step markerless genome integration in E. coli
    Depositor
    Ryan Gill
    Article
    Bassalo et al ACS Synth Biol. 2016 Apr 22.
    Insert
    gfpuv
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    T7A1
    Available since
    Jan. 22, 2016
    Availability
    Academic Institutions and Nonprofits only
    71655-plasmid-map-sequence-id-257561

    Enlarge

  11. pBDK

    Plasmid
    #135190
    Purpose
    Integration and excision of kanamycin cassette for the E. coli genome via CreI, SceI, and lamba Red system. Contains SacB selection
    Depositor
    Hongqing Fang
    Article
    Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d
    Insert
    KanR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    cat
    Available since
    March 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    135190-plasmid-map-sequence-id-273774

    Enlarge

  12. pMAL_dstβL

    Plasmid
    #73805
    Purpose
    Plasmid for selection for insertion and homodimerization
    Depositor
    Sarel Fleishman
    Article
    Elazar et al Elife. 2016 Jan 29;5. pii: e12125. doi:
    Inserts
    CAT Marker
    ToxR
    beta lactamase
    spectomycin resistance
    CLS TM domain
    Use
    Tags
    Part Of the dstbl chiemra, Part of the dsTbL chim…
    Expression
    Bacterial
    Mutation
    Promoter
    NaN and ToxR promotor
    Available since
    March 14, 2016
    Availability
    Academic Institutions and Nonprofits only

  14. pHL 1075

    Plasmid
    #53004
    Purpose
    pCon:fhlA::mCherry, pLlacO-1:sodB::gfp
    Depositor
    Han Lim
    Article
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    fhlA
    sodB
    Use
    Synthetic Biology
    Tags
    GFP and mCherry
    Expression
    Bacterial
    Mutation
    contains −309 to +96 bp relative to start codon a…
    Promoter
    pCon and pLlacO-1
    Available since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  17. pKL001

    Plasmid
    #98917
    Purpose
    Constitutively expresses the CaPR construct, the voltage indicator PROPS tethered to the calcium sensor GCaMP6f, under the Sigma 70 promoter in E. coli.
    Depositor
    Joel Kralj
    Article
    Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11
    Insert
    CaPR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    BBa_J23118 - Strong Constitutive
    Available since
    Aug. 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    98917-plasmid-map-sequence-id-189879

    Enlarge

  20. pDEINO3

    Plasmid
    #179487
    Purpose
    Multi-host shuttle plasmid for E. coli, D. radiodurans, and S. cerevisiae; contains an oriT for transfer via conjugation
    Depositor
    Bogumil Karas
    Article
    Brumwell et al ACS Synth Biol. 2022 Mar 18;11(3):1068-1
    Insert
    TetR/A
    Use
    Synthetic Biology; Bacterial and yeast cloning
    Tags
    Expression
    Mutation
    Promoter
    Available since
    May 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    179487-plasmid-map-sequence-id-360433

    Enlarge

  21. pCDF-NtXNtS1R1R2B2

    Plasmid
    #160882
    Purpose
    Expression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1, bsd2 and raf2 in E. coli.
    Depositor
    Maureen Hanson
    Article
    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do
    Insert
    rbcS-S1, rbcX, raf1, raf2, bsd2
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    rbcS-S1: T7, chaperones: T7
    Available since
    Dec. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    160882-plasmid-map-sequence-id-318842

    Enlarge

  24. pASKt15C+SrtA7

    Plasmid
    #64986
    Purpose
    Expresses the P94R/E105K/E108A/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/
    Insert
    sortase A
    Use
    Tags
    His6 tag and S tag
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-59, P94R, E105K, E108A, D16…
    Promoter
    tetracycline promoter/operator
    Available since
    June 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    64986-plasmid-map-sequence-id-109956

    Enlarge

  29. pANY6

    Plasmid
    #112198
    Purpose
    This plasmid is used for efficient cloning of DNA, efficient expression of protein in E. coli, and ELP-based protein purification.
    Depositor
    Yingfeng An
    Article
    Gao et al J Agric Food Chem. 2018 Jun 20;66(24):61
    Type
    Empty backbone
    Use
    Tags
    ELP-tag
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 24, 2018
    Availability
    Academic Institutions and Nonprofits only
    112198-plasmid-map-sequence-id-218116

    Enlarge

  33. pASKt15C+SrtA5

    Plasmid
    #64985
    Purpose
    Expresses the P94R/D160N/D165A/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/
    Insert
    sortase A
    Use
    Tags
    His6 tag and S tag
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-59, P94R, D160N, D165A, K19…
    Promoter
    tetracycline promoter/operator
    Available since
    June 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    64985-plasmid-map-sequence-id-109954

    Enlarge

  37. pASKt15C+SrtA7woH

    Plasmid
    #65020
    Purpose
    Expresses the P94R/E105K/E108A/D160N/D165K/K190E/K196T mutant of Staphylococcus aureus sortase A in E. coli
    Depositor
    Teruyuki Nagamune
    Article
    Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/
    Insert
    sortase A
    Use
    Tags
    S tag
    Expression
    Bacterial
    Mutation
    deleted amino acids 1-59, P94R, E105K, E108A, D16…
    Promoter
    tetracycline promoter/operator
    Available since
    June 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    65020-plasmid-map-sequence-id-109964

    Enlarge

Showing: 1551 - 1600 of 9150 results