Addgene

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EMM71T

Plasmid

#49044

Purpose

Backbone contains SHD/T 0.5 Domain TALE N-terminal TALE C-terminus, LSD1: Isoform 2

Depositor

Bradley Bernstein, Eric Mendenhall

Article

Mendenhall et al Nat Biotechnol. 2013 Sep 8. doi: 10.1038

Type

Empty backbone

Use

Tale lsd1 expression vector

Tags

Expression

Mammalian

Mutation

Promoter

EF1 alpha

Available since

Nov. 5, 2013

Availability

Academic Institutions and Nonprofits only

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Dyn2-pmCherryN1

Plasmid

#27689

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

Dynamin 2 (DNM2 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

May 4, 2011

Availability

Academic Institutions and Nonprofits only

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BIN1-pmCherryN1

Plasmid

#27693

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

BIN1 (Bin1 Mouse)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

May 4, 2011

Availability

Academic Institutions and Nonprofits only
Epsin2-pmCherryC1

Plasmid

#27673

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

Epsin 2 (Epn2 Mouse)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

May 4, 2011

Availability

Academic Institutions and Nonprofits only

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pLVXPuro-mGFP-Sec23B

Plasmid

#66598

Purpose

Expresses Sec23B as a fusion with mGFP

Depositor

David Stephens

Article

Hughes et al J Cell Sci. 2010 Dec 1;123(Pt 23):4032-8

Insert

Sec23B (SEC23B Human)

Use

Lentiviral

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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BiP-mGFP P495L

Plasmid

#62232

Purpose

Mutant of hamster BiP-mGFP unable to bind clients.

Depositor

Erik Snapp

Article

Lai et al Mol Biol Cell. 2010 Jun 15;21(12):1909-2

Insert

hamster BiP-mGFP-KDEL

Use

Tags

mGFP

Expression

Mammalian

Mutation

Promoter

cmv

Available since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pGagMBPHis

Plasmid

#42176

Depositor

James Hurley

Article

Carlson et al Proc Natl Acad Sci U S A. 2012 Oct 16;10

Insert

Gag protein sequence is from HIV-1 clone NL4-3

Use

Tags

6xHIS and MBP

Expression

Bacterial

Mutation

Promoter

Available since

July 25, 2013

Availability

Academic Institutions and Nonprofits only
HA-GFP_rescue_construct

Plasmid

#190641

Purpose

Puromycin-selectable expression of GFP in Drosophila S2 cells

Depositor

David Bartel

Article

Kingston et al Mol Cell. 2022 Sep 15. pii: S1097-2765(2

Insert

EGFP

Use

Tags

3xHA

Expression

Insect

Mutation

Promoter

Actin-5c

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pPOTv7-hygro-BFP

Plasmid

#190260

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Sue Vaughan

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

BFP

Use

For endogenous gene tagging

Tags

Expression

Mutation

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pPOTv7-hygro-mCherry

Plasmid

#190262

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Sue Vaughan

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mCherry

Use

For endogenous gene tagging

Tags

Expression

Mutation

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOTv7-blast-BFP

Plasmid

#190258

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Sue Vaughan

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

BFP

Use

For endogenous gene tagging

Tags

Expression

Mutation

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOTv7-g418-BFP

Plasmid

#190259

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Sue Vaughan

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

BFP

Use

For endogenous gene tagging

Tags

Expression

Mutation

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOTv7-g418-mCherry

Plasmid

#190261

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Sue Vaughan

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mCherry

Use

For endogenous gene tagging

Tags

Expression

Mutation

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPOTv7-hygro-mScarlet

Plasmid

#190263

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Sue Vaughan

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mScarlet

Use

For endogenous gene tagging

Tags

Expression

Mutation

Promoter

read through

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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HA-mRIN2 dRA

Plasmid

#108941

Purpose

Mammalian expression of mRIN2 dRA

Depositor

Guido Serini

Article

Sandri et al Cell Res. 2012 Oct;22(10):1479-501. doi:

Insert

Ras and Rab interactor 2 (Rin2 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Deletion of RA domain (aa 751-836)

Promoter

CMV

Available since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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HA-mRIN2 dRHVPS9

Plasmid

#108942

Purpose

Mammalian expression of mRIN2 dRHVPS9

Depositor

Guido Serini

Article

Sandri et al Cell Res. 2012 Oct;22(10):1479-501. doi:

Insert

Ras and Rab interactor 2 (Rin2 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Deletion of RHVPS9 domain (aa 455-738)

Promoter

CMV

Available since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

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Nbr_C_sgRNA2

Plasmid

#186664

Purpose

Nbr C-tag sgRNA2 plasmid

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

Nbr sgRNA 2 Plasmid (Nbr Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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Cas9_YTHDF2_sgRNA

Plasmid

#186673

Purpose

YTHDF2 sgRNA plasmid

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

YTHDF2 KO sgRNA Plasmid (YTHDF2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS-RaichuEV-Rac1(Y40C)

Plasmid

#164903

Purpose

Expresses a Rac1 FRET sensor RaichuEV-Rac1 which contains a dominant negative mutation Y40C in the Rac1 domain. More useful than T17N mutation due to less harmful to endogenous signaling.

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

RaichuEV-Rac1 (RAC1 Human)

Use

Tags

Expression

Mammalian

Mutation

Y40C mutation in the Rac1 domain whcih make the s…

Promoter

Available since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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CIB-VP16 (B1014)

Plasmid

#92036

Purpose

Expresses CIB1 (full length, with endogenous NLS) fused to VP16 activation domain.

Depositor

Chandra Tucker

Article

Pathak et al Nucleic Acids Res. 2017 Apr 20. doi: 10.

Insert

CIB1 (CIB1 Mustard Weed)

Use

Tags

Fusion of plant CIB1 (intact NLS) with VP16 activ…

Expression

Mammalian

Mutation

Promoter

T7

Available since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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