We narrowed to 15,231 results for: LEA

1. pLV-H2B-iRFP670-p2a-mCerulean-Geminin (1–110)-IRES-Neomycin

   Plasmid

   #223959

   Purpose

   Dual fluorescent reporter for histone H2B and for APC/C activity

   Depositor

   Hee Won Yang

   Article

   Kim et al Cell Rep. 2023 Nov 28;42(11):113198. doi

   Insert

   H2B-iRFP670-p2a-mCerulean-Geminin (1–110) (GMNN, H2B Human)
   

   Use

   Lentiviral

   Tags

   iRFP670 and mCerulean

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. SPOV strain SM-6 V-1 NS2B-NS3 protease fusion with non cleavable linker

   Plasmid

   #228656

   Purpose

   Bacterial expression of the fusion of the NS2B-NS3 protease from Spondweni virus for biochemical assay

   Depositor

   ASAP Discovery, Frank von Delft

   Insert

   Spondweni virus portion of NS2B protease fused to NS3
   

   Use

   Tags

   His-Twin Strep-Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 18, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. POWV strain LB NS2B-NS3 protease fusion with non cleavable linker

   Plasmid

   #228654

   Purpose

   Bacterial expression of the fusion of the NS2B-NS3 protease from Powassan virus for biochemical assay

   Depositor

   ASAP Discovery, Frank von Delft

   Insert

   Powassan virus portion of NS2B protease fused to NS3
   

   Use

   Tags

   His-Twin Strep-Tev

   Expression

   Bacterial

   Mutation

   Alanine mutant to stop self-cleaving ..VEREEA*AL.…

   Promoter

   Available since

   Feb. 18, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pLV-H2B-iRFP670-p2a-mCerulean-Cdt1 (1-100)-IRES-puromycin

   Plasmid

   #223965

   Purpose

   Dual fluorescent reporter for histone H2B and for Cdt1

   Depositor

   Hee Won Yang

   Article

   Zhang et al Nat Commun. 2023 Nov 29;14(1):7847. doi:

   Insert

   H2B-iRFP670-p2a-mCerulean-Cdt1 (1-100) (H2B, CDT1 Human)
   

   Use

   Lentiviral

   Tags

   iRFP670 and mCerulean

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. WESSV strain SAH177 NS2B-NS3 protease fusion with non cleavable linker

   Plasmid

   #228659

   Purpose

   Bacterial expression of the fusion of the NS2B-NS3 protease from Wesselsbron virus for biochemical assay

   Depositor

   ASAP Discovery, Frank von Delft

   Insert

   Wesselsbron virus portion of NS2B protease fused to NS3
   

   Use

   Tags

   His-Twin Strep-Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 18, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. SLEV strain Kern217 NS2B-NS3 protease fusion with non cleavable linker

   Plasmid

   #228657

   Purpose

   Bacterial expression of the fusion of the NS2B-NS3 protease from St. Louis encephalitis virus for biochemical assay

   Depositor

   ASAP Discovery, Frank von Delft

   Insert

   SLEV portion of NS2B protease fused to NS3
   

   Use

   Tags

   His-Twin Strep-Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 18, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. MVEV strain MVE-1-51 NS2B-NS3 protease fusion with non cleavable linker

   Plasmid

   #228653

   Purpose

   Bacterial expression of the fusion of the NS2B-NS3 protease from Murray Valley encephalitis virus for biochemical assay

   Depositor

   ASAP Discovery, Frank von Delft

   Insert

   MVEV portion of NS2B protease fused to NS3
   

   Use

   Tags

   His-Twin Strep-Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 18, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. DENV-4 strain H241 NS2B-NS3 protease fusion with non cleavable linker

   Plasmid

   #228652

   Purpose

   Bacterial expression of the fusion of the NS2B-NS3 protease from Dengue virus serotype 4 for biochemical assays

   Depositor

   ASAP Discovery, Frank von Delft

   Insert

   Dengue 4 portion of NS2B protease fused to NS3
   

   Use

   Tags

   His-Twin Strep-Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 18, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pJFRC81-3xVAS1-Syn21-Citrine-HA-P10-3xVAS4-Syn21-FLAG-Cerulean-P10

   Plasmid

   #104619

   Purpose

   Expresses HA tagged Citrine or FLAG tagged Cerulean upon binding of TALE1 to VAS1 or TALE4 to VAS4

   Depositor

   Tudor Fulga

   Article

   Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

   Insert

   3xVAS4-hsp70-Syn21-FLAG-Cerulean-P10
   

   Use

   Tags

   FLAG

   Expression

   Insect

   Mutation

   Promoter

   hsp70

   Available since

   March 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pIRES-mCerulean-FRB-RhoA/N12-RhoA/13C(Q63L)-FKBP-mVenus-CAAX

    Plasmid

    #214282

    Purpose

    Express split-RhoA fragments that are fused with CIDs and FPs

    Depositor

    Cliff Stains

    Article

    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

    Inserts

    Split-RhoA fragments fused with CIDs and FPs (RHOA Human)
    ras homolog family member A (RHOA Human)
    

    Use

    Tags

    mCerulean (on insert 1) and mVenus (on insert 2)

    Expression

    Mammalian

    Mutation

    RhoA-Q63L

    Promoter

    CMV

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. EV-A71 3C protease; aliases: Enterovirus A71 3C protease, non cleavable C-term 6xHis tag

    Plasmid

    #204816

    Purpose

    Bacterial expresison of Enterovirus A71 3C protease

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    EV A71 3C protease
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 17, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. EV-D68 3C protease; aliases: Enterovirus A71 3C protease, non cleavable C-term 6xHis tag

    Plasmid

    #204817

    Purpose

    Bacterial expresison of Enterovirus D68 3C protease

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    EV D68 3C protease
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pIRES-mCerulean-FRB-Cdc42/N12-Cdc42/13C(Q61L)-FKBP-mVenus-CAAX

    Plasmid

    #214278

    Purpose

    Express split-Cdc42 fragments that are fused with CIDs and FPs

    Depositor

    Cliff Stains

    Article

    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

    Inserts

    Split-Cdc42 fragments fused with CIDs and FPs (CDC42 Human)
    Split-Cdc42 fragments fused with CIDs and FPs (CDC42 Human)
    

    Use

    Tags

    mCerulean (on insert 1) and mVenus (on insert 2)

    Expression

    Mammalian

    Mutation

    Cdc42-Q61L

    Promoter

    CMV

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTol2-Hsp70-H2B-mCerulean-lsl-mScarlet-fli1-zCre-I-BFP (JDW 1233)

    Plasmid

    #229841

    Purpose

    A Tol2 based expression vector with the Hsp70 promoter driving a cre dependent switch reporter. Contains an endothelial driven cre

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    loxp-H2B-mCerulean-2xStop-loxP
    

    Use

    Cre/Lox; Tol2 based expression vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. USUV strain Vienna 2001 NS2B-NS3 protease fusion with non cleavable linker

    Plasmid

    #228658

    Purpose

    Bacterial expression of the fusion of the NS2B-NS3 protease from Usutu virus for biochemical assay

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    Usutu virus portion of NS2B protease fused to NS3
    

    Use

    Tags

    His-Twin Strep-Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 18, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. ROCV strain SPH 34675 NS2B-NS3 protease fusion with non cleavable linker

    Plasmid

    #228655

    Purpose

    Bacterial expression of the fusion of the NS2B-NS3 protease from Rocio virus for biochemical assay

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    Rocio virus portion of NS2B protease fused to NS3
    

    Use

    Tags

    His-Twin Strep-Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 18, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. DENV-2 strain Thailand/16681/84 NS2B-NS3 protease fusion with non cleavable linker

    Plasmid

    #228650

    Purpose

    Bacterial expression of the fusion of the NS2B-NS3 protease from Dengue virus serotype 2 for biochemical assays

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    Dengue 2 portion of NS2B protease fused to NS3
    

    Use

    Tags

    His-Twin Strep-Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 18, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. DENV-1 strain US/Hawaii/1944 NS2B-NS3 protease fusion with non cleavable linker

    Plasmid

    #228648

    Purpose

    Bacterial expression of the fusion of the NS2B-NS3 protease from Dengue virus serotype 1 for biochemical assay

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    Dengue 1 portion of NS2B protease fused to NS3
    

    Use

    Tags

    His-Twin Strep-Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 18, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pIRES-mCerulean-FRB-Cdc42/N12-Cdc42/13C(T17N)-FKBP-mVenus-CAAX

    Plasmid

    #214279

    Purpose

    Express split-Cdc42 fragments that are fused with CIDs and FPs

    Depositor

    Cliff Stains

    Article

    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

    Inserts

    Split-Cdc42 fragments fused with CIDs and FPs (CDC42 Human)
    Split-Cdc42 fragments fused with CIDs and FPs (CDC42 Human)
    

    Use

    Tags

    mCerulean (on insert 1) and mVenus (on insert 2)

    Expression

    Mammalian

    Mutation

    Cdc42-T17N

    Promoter

    CMV

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pIRES-mCerulean-FRB-Rac1/N12-Rac1/13C(Q61L)-FKBP-mVenus-CAAX

    Plasmid

    #214280

    Purpose

    Express split-Rac1 fragments that are fused with CIDs and FPs

    Depositor

    Cliff Stains

    Article

    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

    Inserts

    Split-Rac1 fragments fused with CIDs and FPs (RAC1 Human)
    Split-Rac1 fragments fused with CIDs and FPs (RAC1 Human)
    

    Use

    Tags

    mCerulean (on insert 1) and mVenus (on insert 2)

    Expression

    Mammalian

    Mutation

    Rac1-Q61L

    Promoter

    CMV

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge