We narrowed to 15,882 results for: LEA
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Plasmid#187993PurposeExpresses MUC2 D1D2D' assembly with cleavable His tag in mammalian cellsDepositorInsertMucin2 D1D2D' (MUC2 Human)
Tags6xHistadine followed by TEV cleavage siteExpressionMammalianPromoterCMVAvailable SinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Pur-mCerulean3-spacer-cpVenus[E172]
Plasmid#125204PurposeSB-transposon plasmid for stable expression of mCerulean3-cpVenus[E172] FRET low controlDepositorInsertmCerulean3-spacer-cpVenus[E172]
UseTransposonExpressionMammalianPromoterEF1a/RPBSAAvailable SinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKK-FRET-ORF1-3C-Cerulean_ORF2-TEV-Amber
Plasmid#105806PurposeConcomitant expression of two proteins. One protein expressed with Cerulean, cleavable by 3C; second protein expressed with Amber, cleavable by TEV. Control for protein interaction analysis by FRET.DepositorTypeEmpty backboneUseFlp-in competentTagsORF1: 3C-Cerulean; ORF2: TEV-AmberExpressionMammalianAvailable SinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAB26 (punc-17::GtACR2::mCerulean::βHK::Chrimson)
Plasmid#214889PurposeExpresses the optogenetic tool BiPOLES in cholinergic neurons of C. elegans.DepositorInsertGtACR2_mCerulean_bHK_Chrimson
TagsmCeruleanExpressionWormAvailable SinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAB27 (pmyo-3::GtACR2::mCerulean::βHK::Chrimson)
Plasmid#214890PurposeExpresses the optogenetic tool BiPOLES in BWMs of C. elegans.DepositorInsertGtACR2_mCerulean_bHK_Chrimson
TagsmCeruleanExpressionWormAvailable SinceMarch 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAB31 (punc-47::GtACR2::mCerulean::bHK::Chrimson)
Plasmid#214891PurposeExpresses the optogenetic tool BiPOLES in GABAergic neurons of C. elegans.DepositorInsertGtACR2_mCerulean_bHK_Chrimson
TagsmCeruleanAvailable SinceMarch 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
EV-A71 2A protease; 6xHis-tag, self cleaving
Plasmid#204809PurposeBacterial expresison of Enterovirus A71 2A proteaseDepositorInsertA71 2A protease
ExpressionBacterialAvailable SinceJan. 17, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSems-leader-SNAPf-mEGFPm-IFNAR1(28-557)
Plasmid#192704PurposeExpression of SNAPf and mEGFPm-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopyDepositorInsertIFNAR1 (IFNAR1 Human)
TagsIg k-chain leader sequence, SNAPf-tag, and meGFPm…ExpressionMammalianMutationmeGFPm: gluatmic acid 142 to asparagine and histi…PromoterCMVAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSems-leader-SNAPf-mXFPm-IFNAR1(28-557)
Plasmid#192784PurposeExpression of SNAPf and mXFPm-tagged IFNAR1 at the plasma membrane, e.g. for single molecule fluorescence microscopyDepositorInsertIFNAR1 (IFNAR1 Human)
TagsIg k-chain leader sequence, SNAPf-tag, and mXFPmExpressionMammalianMutationmXFPm: tryptophan 66 to phenylalanine, gluatmic a…PromoterCMVAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/(CAA)26 5′ leader GUG-nLuc-3XFLAG
Plasmid#127325PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from GUG start codon from mRNA with (CAA)26 repeats in 5' UTRDepositorInsertnanoLuciferase
Tags3xFLAGExpressionMammalianPromoterCMVAvailable SinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/(CAA)26 5′ leader AUG-nLuc-3XFLAG
Plasmid#127324PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with (CAA)26 repeats in 5' UTRDepositorInsertnanoLuciferase
Tags3xFLAGExpressionMammalianPromoterCMVAvailable SinceJune 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJFRC81-3xVAS4-Syn21-FLAG-Cerulean-P10
Plasmid#104618PurposeExpresses FLAG tagged Cerulean upon binding of TALE4 to VAS4DepositorInserthsp70-Syn21-FLAG-mCerulean-P10
TagsFLAGExpressionInsectPromoterhsp70Available SinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_HA-p115-TEV-cleaved #957#382
Plasmid#42107DepositorInsertp115
TagsHAExpressionMammalianMutationdeleted 886 to end and added ENLYFQPromoterCMVAvailable SinceFeb. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Cerulean-Epac2(R447)-cpV[E172]
Plasmid#138198PurposeFRET-based Epac2 activation sensor with R447A mutation in Epac2 coding sequence.DepositorInsertCerulean-Epac2(R447A)-cpVenus[E172] (Rapgef4 Synthetic, Mouse)
TagsCerulean and cpV[E172]ExpressionMammalianMutationContains arginine-to-alanine substitution at resi…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
PCDNA3-FlipGFP(Casp3 cleavage seq) T2A mCherry
Plasmid#124428PurposeExpresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in mammalian cellsDepositorInsertFlipGFP(Casp3 cleavage seq) T2A mCherry
ExpressionMammalianAvailable SinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
PCDNA3-FlipGFP(TEV cleavage seq) T2A mCherry
Plasmid#124429PurposeExpresses FlipGFP (TEV cleavage sequence) and T2A mCherry in mammalian cellsDepositorInsertFlipGFP(TEV cleavage seq) T2A mCherry
ExpressionMammalianAvailable SinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
PCDNA3-FlipGFP(Caspase-1 cleavage seq) T2A mCherry
Plasmid#124494PurposeExpresses FlipGFP (caspase-1 cleavage sequence) and T2A mCherryDepositorInsertFlipGFP(Caspase-1 cleavage seq) T2A mCherry
UseSynthetic BiologyAvailable SinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
PCDNA3-FlipYFP(TEV cleavage seq) T2A mCherry
Plasmid#124493PurposeExpresses FlipYFP (TEV cleavage sequence) and T2A mCherryDepositorInsertFlipYFP(TEV cleavage seq) T2A mCherry
UseSynthetic BiologyAvailable SinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIHEU-FlipGFP(Casp3 cleavage seq) T2A mCherry
Plasmid#124434PurposeExpresses FlipGFP (caspase-3 cleavage sequence) and T2A mCherry in DrosophilaDepositorInsertFlipGFP(Casp3 cleavage seq) T2A mCherry
ExpressionInsectAvailable SinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
PCS2-FlipGFP(Casp3 cleavage seq) T2A mCherry
Plasmid#124431PurposeExpresses FlipGFP (caspase-3 cleavage sequence) protease reporter and T2A mCherry in zebrafishDepositorInsertFlipGFP(Casp3 cleavage seq) T2A mCherry
UseZebrafish (if expression level is too high in zeb…Available SinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only