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HSF1 KO

Plasmid

#200207

Purpose

gRNA for HSF1 KO

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

HSF1 KO (Hsf1 Mouse)

Expression

Mammalian

Mutation

None

Promoter

U6

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pLV Reep4-V5

Plasmid

#175120

Purpose

Lentiviral expression of mouse Reep4-V5

Depositor

Larry Gerace

Article

Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

Insert

Reep4 (Reep4 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV FHA-ATG12

Plasmid

#27049

Depositor

Jayanta Debnath

Article

Radoshevich et al Cell. 2010 Aug 20. 142(4):590-600.

Insert

autophagy-related 12 (yeast) (Atg12 Mouse)

Use

Retroviral

Tags

HA and flag

Expression

Mammalian

Available Since

Jan. 13, 2011

Availability

Academic Institutions and Nonprofits only
pLNC Wnt-6HA

Plasmid

#18035

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-6 (Wnt6 Mouse)

Use

Retroviral

Tags

HA

Available Since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
Pdx-PH2/pGL3

Plasmid

#12163

Depositor

Domenico Accili

Article

Kitamura et al J Clin Invest. 2002 Dec . 110(12):1839-4

Insert

Pdx1 promoter PH2 domain

Use

Luciferase

Available Since

June 13, 2007

Availability

Academic Institutions and Nonprofits only
pCAG eGFP-Oct6

Plasmid

#203790

Purpose

Competitive assay of Oct4 and Oct6 binding to DNA motifs.

Depositor

Thorsten Wohland

Article

Mistri et al EMBO Rep. 2015 Sep;16(9):1177-91. doi: 1

Insert

Pou3f1 (Pou3f1 Mouse)

Tags

mEGFP

Expression

Mammalian

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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Venus-BimL-pEGFP-C1

Plasmid

#166734

Purpose

Express Venus fused to the N-terminus of the Bcl-2 family protein, BimL

Depositor

David Andrews

Article

Liu et al Elife. 2019 Mar 12;8. pii: 37689. doi: 1

Insert

BimL (Bcl2l11 Mouse)

Tags

Venus

Expression

Mammalian

Promoter

CMV

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pQE60-mPU.1-ETS

Plasmid

#85733

Purpose

Expresses DNA-binding (ETS) domain of murine PU.1, residues 167-272, in M15 cells already pre-transformed with 2nd plasmid, pREP-4

Depositor

Gregory Poon

Article

Poon et al J Biol Chem. 2012 May 25;287(22):18297-3

Insert

ETS domain of transcription factor PU.1 (Spi1 Mouse)

Tags

6xHis tag

Expression

Bacterial

Available Since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2-HA-Mettl3

Plasmid

#196262

Purpose

In vitro transcription/translation of HA-tagged wild-type Mettl3

Depositor

Laura Attardi

Article

Raj et al Mol Cell. 2022 Jul 7;82(13):2370-2384.e1

Insert

Mettl3 (Mettl3 Mouse)

Tags

3xHA

Expression

Mammalian

Promoter

CMV IE94

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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isoform PKC Delta (JCE594)

Plasmid

#182025

Purpose

yeast expression of mouse PKC Delta with yeast PKC1 promoter and ADH1 terminator

Depositor

Juan Carlos Igual

Insert

PKC Delta (Prkcd Mouse)

Expression

Mammalian

Promoter

yeast PKC1 promoter (-600 to -1)

Available Since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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pChuk

Plasmid

#87033

Purpose

express IKKalpha in mammalian cells

Depositor

Georgios Stathopoulos

Article

Marazioti et al Nat Commun. 2018 Feb 14;9(1):672. doi: 1

Insert

IKKalpha (Chuk Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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Anti-GABA(A)R, Gamma2L [N452/81R]

Plasmid

#177554

Purpose

Mammalian Expression Plasmid of anti-GABA(A)R, Gamma2L (Human). Derived from hybridoma N452/81.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GABA(A)R, Gamma2L (Homo sapiens) recombinant mouse monoclonal antibody (GABRG2 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pId1-IRES-YFP

Plasmid

#22667

Depositor

Robert Benezra

Article

Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

Insert

Inhibitor of DNA binding 1 (Id1 Mouse)

Tags

IRES-YFP

Expression

Mammalian

Available Since

Jan. 20, 2010

Availability

Academic Institutions and Nonprofits only

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eGFP-KIF5A-Rigor

Plasmid

#172202

Purpose

Expresses KIF5A G235A rigor mutant tagged with eGFP in mammalian cells

Depositor

Juan Bonifacino

Article

Farias et al Cell Rep. 2015 Nov 10;13(6):1221-1232. d

Insert

KIF5A (Kif5a Mouse)

Tags

eGFP

Expression

Mammalian

Mutation

Changed Glycine 235 to Alanine

Promoter

CMV

Available Since

July 26, 2021

Availability

Academic Institutions and Nonprofits only

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pT7[mRNA]-5'UTR:mMyod1[NM_010866.2]: Hba-a1_3'UTR

Plasmid

#205026

Purpose

In vitro transcription vector for production of synthetic mRNA encoding for mouse Myod1

Depositor

Ori Bar-Nur

Article

Qabrati et al NPJ Regen Med. 2023 Aug 8;8(1):43. doi:

Insert

Myod1 (Myod1 Mouse)

Use

Synthetic Biology; In vitro transcription vector …

Available Since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5 FLAG membrane-bound lipin-1 (TMD)

Plasmid

#120277

Purpose

Expression of ER/Golgi-tethered lipin-1 in mammalian cells

Depositor

Sirio Dupont

Article

Romani et al Nat Cell Biol. 2019 Mar;21(3):338-347. d

Insert

Lipin-1 (Lpin1 Mouse)

Tags

FLAG and in-frame fusion with the TMD Golgi-local…

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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tdEos-Beta-Catenin-20

Plasmid

#57586

Purpose

Localization: Tight Junctions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Insert

Beta-Catenin (Ctnnb1 Mouse)

Tags

tdEos

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2015

Availability

Industry, Academic Institutions, and Nonprofits
KA1869_pGL4-UBCmin-luc2/Fgf4-A'

Plasmid

#124195

Purpose

Luciferase reporter driven by the Fgf4 enhancer

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Insert

Fgf4 enhancer

Use

Luciferase

Promoter

UBCmin

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti-sgNotch1#1/Cre

Plasmid

#193223

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Notch1 gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgNotch1#1 (Notch1 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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Kir6.2 K+ channel scFv [N363/71]

Plasmid

#190546

Purpose

Mammalian Expression of Kir6.2 K+ channel scFV. Derived from hybridoma N363/71.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Kir6.2 K+ channel (Rattus norvegicus) recombinant scFV (Kcnj11 Mouse)

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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