174,739 results
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Plasmid#180264PurposeLentiviral expression vector for dCas9-KRAB in mammalian cellsDepositorInsertdCas9
UseCRISPR and LentiviralTagsKRAB and mCherryExpressionMammalianMutationNuclease dead Cas9PromoterSFFVAvailable SinceFeb. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-28b-RfxCas13d-His
Plasmid#141322PurposePlasmid for bacterial expression and purification of RfxCas13d proteinDepositorInsertRfx-Cas13d
UseCRISPRTags6xHisExpressionBacterialPromoterT7Available SinceAug. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
Mm-PylRS-AF/Pyl-tRNACUA
Plasmid#122650PurposetRNA synthetase/tRNA pair for the incorporation of unnatural amino acid into proteins in mammalian cells in response to the amber codon TAG.DepositorInsertPylRS-AF, Pyl-tRNACUA
TagsFLAGExpressionMammalianMutationTyrosine 306 changed to Alanine, Tyrosine 384 cha…Available SinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-pCMV-PfV-GS-Sapphire
Plasmid#116933PurposeMammalian vector for expression of the 40nm GEMDepositorInsertPfv
TagsSapphireExpressionMammalianPromoterCMVAvailable SinceDec. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
T444T
Plasmid#113081PurposeEnhanced RNAi vector for C. elegans. Contains two T7 polymerase termination sequences adjacent to the T7 promoter sites.DepositorTypeEmpty backboneUseRNAiExpressionBacterialAvailable SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti-FNLS-P2A-Puro
Plasmid#110841PurposeLentiviral vector for constitutive expression of FNLS in mammalian cells (codon optimized)DepositorInsertBE3RA-FNLS
UseLentiviralTags3X FLAGMutationD10A and NLS sequence at the N-terminusPromoterEF1sAvailable SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLentiPGK DEST H2B-iRFP670
Plasmid#90237PurposeLentiviral vector to express H2B-iRFP670 under PGK promoterDepositorInsertH2B
UseLentiviralTagsiRFP670ExpressionMammalianPromoterPGKAvailable SinceMarch 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTP943
Plasmid#104157PurposeBacterial expression plasmid of anti-mouse IgG1 Fab nanobody TP886 (3x Cysteines)DepositorInsertAnti-mouse IgG1 Fab specific nanobody TP886
Tags14xHistidine tag and NEDD8 from Brachypodium dist…ExpressionBacterialAvailable SinceDec. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
TRE-KRAB-dCas9-IRES-GFP
Plasmid#85556Purpose2nd generation lentiviral vector expressing a KRAB-dCas9 fusion protein and GFPDepositorInsertKRAB-dCas9-IRES-GFP
UseCRISPR and LentiviralTags2x NLS, HA Tag, and KRAB domainExpressionMammalianPromoterTRE3GAvailable SinceSept. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-AR V7
Plasmid#86856PurposeFluorescent human androgen-receptor splice variant 7, lacking the ligand-binding domain (fused to EGFP)DepositorInsertAndrogen receptor (AR) (AR Human)
TagsEGFPExpressionMammalianMutationAlternative splice variant 7 (alteration/deletion…PromoterCMVAvailable SinceApril 19, 2017AvailabilityAcademic Institutions and Nonprofits only