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We narrowed to 9,854 results for: EPO

Showing: 9601 - 9630 of 9854 results
  1. JDW 859 (pTol2-kdrl:V5-mScarlet-I-hsKRAS4A-G12V-ac/mC)

    Plasmid
    #156416
    Purpose
    kdrl promoter driving V5-tagged mScarlet-I fused to human KRAS4A-G12V with an alpha-crystallin mCerulean reporter flanked by Tol2 sites
    Depositor
    Insert
    human mutant KRAS4A-G12V (KRAS Human)
    Use
    Zebrafish transgenesis
    Tags
    mScarlet-I
    Expression
    Mutation
    mutant KRAS4A-G12V
    Promoter
    kdrl
    Available Since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
  2. pGL4.23 C5_15

    Plasmid
    #60322
    Purpose
    This plasmid contains a genomic fragment that is not active in human pancreatic islets, a minimal promoter and a luc2 gene. Can be used as negative control in reporter assays performed in human pancreatic islets.
    Depositor
    Insert
    Non active element (nearest TSS PHLDA1) (PHLDA1 Human)
    Use
    Luciferase
    Tags
    Luciferase
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Dec. 2, 2014
    Availability
    Academic Institutions and Nonprofits only
  3. pLY61

    Plasmid
    #130928
    Purpose
    A CRISPR activation device with the necessary genes (dxcas9 3.7 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA1B1 with sfgfp::ASV).
    Depositor
    Inserts
    dxcas9 (3.7)
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    Use
    Synthetic Biology
    Tags
    ASV tag and pspFΔHTH::λN22plus
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9 for dCas9; Sy…
    Promoter
    Anderson promoter: J23106, PpspA-LEA1B1, and Ptet
    Available Since
    Sept. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
  4. JDW 770 (pTol2-kdrl:EGFP-hsKRAS4B-G12V-ac/Y)

    Plasmid
    #156414
    Purpose
    kdrl promoter driving EGFP fused to human KRAS4B-G12V with an alpha-crystallin Venus (YFP) reporter flanked by Tol2 sites
    Depositor
    Insert
    human mutant KRAS4B-G12V (KRAS Human)
    Use
    Zebrafish transgenesis
    Tags
    EGFP
    Expression
    Mutation
    mutant KRAS4B-G12V
    Promoter
    kdrl
    Available Since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
  5. pCS2-NES-Myc(340-439)-GFP-IRES-mCherry (NES-DBD)

    Plasmid
    #231237
    Purpose
    MYC stability reporter construct of the DNA-binding-domain (residues 340-439) fused to an N-terminal NES for transient expression in mammalian cells
    Depositor
    Insert
    MYC (MYC Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    NES fused to the DNA-binding-domain of MYC (resid…
    Promoter
    Available Since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
  6. pCS2-MYC(Δ1-143; Δ340-439)-GFP-IRES-mCherry (ΔTADΔDBD)

    Plasmid
    #231235
    Purpose
    MYC stability reporter construct with deletion of the N-terminal region (residues 1-143) and DNA-binding-domain (residues 340-439) for transient expression in mammalian cells
    Depositor
    Insert
    MYC (MYC Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    TAD deleted (delta1-143), DBD deleted (delta340-4…
    Promoter
    Available Since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
  7. pCS2-NLS-Myc(340-439)-GFP-IRES-mCherry (NLS-DBD)

    Plasmid
    #231236
    Purpose
    MYC stability reporter construct of the DNA-binding-domain (residues 340-439) fused to an N-terminal NLS for transient expression in mammalian cells
    Depositor
    Insert
    MYC (MYC Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    NLS fused to the DNA-binding-domain of MYC (resid…
    Promoter
    Available Since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
  8. pBactin-DIO-inv[roxSTOProx-EB3-mNeonGreen-bGH]

    Plasmid
    #196887
    Purpose
    Dual Cre/Dre-dependent expression of EB3-mNeon. Used as functional (labelling of microtubule plus ends) Cre-Dre cotransfection reporter
    Depositor
    Insert
    roxSTOProx-EB3 mNeonGreen-bGH (MAPRE3 Human)
    Use
    Cre/Lox; Dre/rox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Chicken bactin (plus Chicken bactin intron)
    Available Since
    Aug. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
  9. pLenti NLuc398-G8NCRD IRES G8NCRD-CLuc394

    Plasmid
    #128386
    Purpose
    Expresses a split firefly luciferase reporter based on the N terminal carbohydrate recognition domain of human galectin 8, which concentrates inside endosomes following endosomal disruption
    Depositor
    Inserts
    NLuc-3xGGGGS-Gal8-NCRD (LGALS8 Synthetic, Human)
    Gal8-NCRD-3xGGGGS-CLuc398 (LGALS8 Human)
    Use
    Lentiviral, Luciferase, and Synthetic Biology
    Tags
    C-terminal luc2 fragment and N-terminal luc2 frag…
    Expression
    Mammalian
    Mutation
    N terminal carbohydrate recognition domain fused …
    Promoter
    EF1A and IRES
    Available Since
    Jan. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
  10. pEN435 - pCAGGS-TagBFP-hGeminin-2A-mCherry-hCdt1-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt Tigre targeting

    Plasmid
    #92139
    Purpose
    Targeting vector to introduce a Fucci cell cyle reporter cassette mCherry(G1)/BFP(S/G2) at the mouse TIGRE acceptor locus using puromycin selection
    Depositor
    Insert
    chromatin licensing and DNA replication factor 1 (GMNN, CDT1 Human)
    Use
    Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
  11. AIMTOR A757

    Plasmid
    #140829
    Purpose
    AIMTOR BRET Biosensor containing a mutated non-phosphorylable ULK1 peptide to use as a control constuct in parrallel with AIMTOR T757
    Depositor
    Insert
    AIMTOR A757 (ULK1 Human)
    Use
    Tags
    NES Nuclear export signal
    Expression
    Mammalian
    Mutation
    The insert comprises only a small sequence of hum…
    Promoter
    Available Since
    July 10, 2020
    Availability
    Academic Institutions and Nonprofits only
  12. pNJP

    Plasmid
    #115494
    Purpose
    Expression vector for NLS-iRFP-NLS, JNK KTR-mCherry, and mKO-MK2 in mammalian cells
    Depositor
    Insert
    NLS-iRFP-NLS-P2A-JNK KTR-mCherry-P2A-mKO-MK2 (MAPK14, MAPK8 Synthetic, Human, Mouse)
    Use
    Tags
    iRFP, mCherry, and mKO
    Expression
    Mammalian
    Mutation
    mCherry S227F, mCherry G229R, mKO V211G
    Promoter
    CAG
    Available Since
    Dec. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
  13. pCDNA3-Flag-SPRTN-E112A

    Plasmid
    #110215
    Purpose
    Expression in mammalian cells of of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, and Flag-tag at N-terminus.
    Insert
    SPRTN (SPRTN Human)
    Use
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    E112A, P296L (see depositor comments below)
    Promoter
    Available Since
    May 23, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 9601 - 9630 of 9854 results