We narrowed to 17,098 results for: igh

Showing: 9601 - 9630 of 17098 results

Results per page:

Sort results by:

pSL1749 (pDonor, R*MmeI)

Plasmid

#160733

Purpose

Mini-transposon donor plasmid for V. cholerae CAST system. Mini-transposon has MmeI site in R end for Tn-seq. pUC19 vector backbone.

Depositor

Samuel H. Sternberg

Article

Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

Insert

VchCAST donor DNA

Expression

Bacterial

Promoter

N/A

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
AB.pCCL.sin.cPPT.GFP.miR-17-3p.sensor.PGK.dNGFR.WPRE

Plasmid

#85866

Purpose

Sensor Plasmid

Depositor

Brian Brown

Article

Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

Insert

miR-17-3p Sensor (MIR17 Human)

Use

Lentiviral

Promoter

PGK

Available Since

Jan. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDGE334

Plasmid

#153243

Purpose

M2E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.CAGFLEX.(cyto).iATPSnFR2.A95A.A119L.HaloTag

Plasmid

#209657

Purpose

Expression of iATPSnFR2, low affinity

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95A.A119L.HaloTag

Use

AAV

Mutation

A95A.A119L

Promoter

CAG

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Bxb1-GA_AIO-TetOn_mScarlet_Divergent

Plasmid

#229791

Purpose

Bxb1-GA donor plasmid with divergent syntax for all-in-one doxycycline-inducible mScarlet expression

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

TRE-mScarlet; rtTA-T2A-mTagBFP2

Use

Synthetic Biology

Tags

NLS

Expression

Mammalian

Promoter

TRE and CAG

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-eNme2-C.NR

Plasmid

#185672

Purpose

Expresses nuclease active eNme2-C.NR in mammalian cells

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

eNme2-C.NR nuclease

Use

CRISPR

Expression

Mammalian

Mutation

eNme2-C S6P/G33E/A520E/S646R/V696F/R711G/V758I/Y7…

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Bxb1-GA_CAG-ITPKA-3x-mScarlet-I

Plasmid

#229782

Purpose

Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of a mScarlet-I F-actin reporter (3 copies)

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

3x mScarlet-I

Use

Synthetic Biology

Tags

ITPKA

Expression

Mammalian

Promoter

CAG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV.hSynapsin.(cyto).iATPSnFR2.A95A.A119L.HaloTag

Plasmid

#209665

Purpose

Expression of iATPSnFR2, low affinity

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95A.A119L.HaloTag

Use

AAV

Mutation

A95A.A119L

Promoter

hSyn

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV_NFL(WT)-FLAG

Plasmid

#182654

Purpose

Expresses wild-type mouse neurofilament light chain with a C-terminal FLAG tag (DYKDDDDK) in mammalian cells.

Depositor

Ivana Nikić-Spiegel

Article

Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

Insert

wild type mouse neurofilament light chain (Nefl Mouse)

Tags

FLAG tag (DYKDDDDK)

Expression

Mammalian

Promoter

CMV

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB511

Plasmid

#74853

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with FLAG (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD/His-B-iRFP

Plasmid

#31855

Purpose

a phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imaging

Depositor

Vladislav Verkhusha

Article

Filonov et al Nat Biotechnol. 2011 Jul 17. doi: 10.103

Insert

iRFP

Expression

Bacterial

Mutation

S13L, A92T, V104I, V114I, E161K, Y193K, F198Y, D2…

Promoter

araBAD

Available Since

Sept. 16, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-CibN-dCas9-CibN

Plasmid

#60553

Purpose

Expresses CibN-dCas9-CibN in mammalian cells

Depositor

Charles Gersbach

Article

Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

Insert

dCas9-CibN

Use

CRISPR

Tags

FLAG and SV40 NLS

Expression

Mammalian

Mutation

inactivating Cas9 mutations D10A and H840A

Promoter

CMV

Available Since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pJM100 (pUC18T-miniTn7T-gm-araC-ParaBAD)

Plasmid

#110554

Purpose

miniTn7 delivery plasmid with araC-ParaBAD inducible promoter

Depositor

Joanna Goldberg

Article

Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

Insert

araC-ParaBAD

Expression

Bacterial

Promoter

AraC-ParaBAD

Available Since

May 23, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-MSP1D1deltaH4-H6

Plasmid

#71717

Purpose

Membrane scaffold protein (MSP), helix 4 to 6 deletion

Depositor

Gerhard Wagner

Article

Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

Insert

Apolipoprotein A1 (APOA1 Human)

Expression

Bacterial

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB404

Plasmid

#74798

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with sGFP (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

No Promoter

Available Since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
mTagBFP2-TFR-20

Plasmid

#55327

Purpose

Localization: Transferin Receptor, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

TFR (TFRC Human)

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pKLV2-EF1adCas9VP64T2ABsd-W

Plasmid

#112919

Purpose

Lentiviral vector expressing Lentiviral vector expressing Lentiviral vector expressing dCas9VP64 under the control of the long human EF1a promoter

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

dCas9VP64-T2A-Blast

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

Human EF1a promoter

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
PB/pHSP-tTA-TRE-tTA-pEF1α-PuroR

Plasmid

#206213

Purpose

PiggyBac-based transposon vector plasmid which encodes an expression unit of tetracycline-responsive transactivator under control of HSP promoter with transcriptional amplification

Depositor

Masamichi Kamihira

Article

Kitano et al Cytotechnology. 2021 Jun;73(3):353-362.

Insert

tetracycline-responsive transactivator

Expression

Mammalian

Available Since

Oct. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
TALEN-CCR5R18-NtermN1

Plasmid

#51445

Purpose

Expresses TALEN targeting right CCR5A site (R18) with N1 N-terminal domain

Depositor

David Liu

Article

Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

Insert

TALEN CCR5 Right (R18) N1 N-term

Use

TALEN

Tags

3xFLAG and Fok1 KK variant

Expression

Mammalian

Mutation

K150Q

Promoter

CMV

Available Since

April 1, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF1-Pls4-sfGFP

Plasmid

#205751

Purpose

Expresses sfGFP under IPTG inducible promoter Pls4 in high copy pTRKH2 gram-positive shuttle vector. Used in Lactobacillus gasseri. High strength.

Depositor

Jerome Bonnet

Article

Fristot et al ACS Synth Biol. 2024 Mar 15;13(3):951-95

Type

Empty backbone

Use

Shuttle vector gram+ gram-

Expression

Bacterial

Promoter

Pls4 (Phyperspank mutant, IPTG inducible)

Available Since

July 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC19 PKC delta

Plasmid

#8427

Depositor

Frederic Mushinski

Article

Mischak et al Biochemistry 1991 Aug 13;30(32):7925-31.

Insert

Mouse Protein Kinase C delta (Prkcd Mouse)

Expression

Bacterial

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pX-evoCas9

Plasmid

#107550

Purpose

Expresses the M495V/Y515N/K526E/R661Q (evoCas9) SpCas9 variant in mammalian cells

Depositor

Anna Cereseto

Article

Casini et al Nat Biotechnol. 2018 Jan 29. pii: nbt.40

Insert

Humanized S. pyogenes Cas9

Use

CRISPR

Tags

3XFLAG

Expression

Mammalian

Mutation

M495V/Y515N/K526E/R661Q

Promoter

CBh

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pShuttle-CMV-iRFP

Plasmid

#31856

Purpose

a phytochrome-based near-infrared fluorescent protein, also known as iRFP713, used for in vivo imaging

Depositor

Vladislav Verkhusha

Article

Filonov et al Nat Biotechnol. 2011 Jul 17. doi: 10.103

Insert

iRFP

Use

Adenoviral

Expression

Mammalian

Mutation

T2A, S13L, A92T, V104I, V114I, E161K, Y193K, F198…

Promoter

CMV

Available Since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
SAP145

Plasmid

#11239

Depositor

Robin Reed

Article

Gozani et al Genes Dev. 1996 Jan 15. 10(2):233-43.

Insert

SAP145 (SF3B2 Human)

Expression

Bacterial

Mutation

First 7 amino acids are missing compared to NP_00…

Available Since

Jan. 19, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
Bxb1-GT_AIO-TetOn_Divergent Donor

Plasmid

#229784

Purpose

Bxb1-GT donor plasmid with divergent syntax for all-in-one doxycycline-inducible expression (empty)

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

LacZ; rtTA-T2A-mTagBFP2

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE and CAG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Bxb1-GA_AIO-TetOn_Divergent Donor

Plasmid

#229786

Purpose

Bxb1-GA donor plasmid with divergent syntax for all-in-one doxycycline-inducible expression (empty)

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

LacZ; rtTA-T2A-mTagBFP2

Use

Synthetic Biology

Expression

Mammalian

Promoter

TRE and CAG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGWB417

Plasmid

#74811

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 4xMyc (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pSPAsp-hp

Plasmid

#48122

Purpose

Shuttle vector E. coli/B.meg.; encoding the predicted optimal signal peptide for protein secretion using Bacillus megaterium under control of the xylose inducible promoter

Depositor

Dieter Jahn

Article

Stammen et al Appl Environ Microbiol. 2010 Jun;76(12):

Insert

predicted optimal signal peptide for Bacillus megaterium protein secretion

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest-521

Plasmid

#159688

Purpose

Gateway destination vector for E. coli protein expression

Depositor

Dominic Esposito

Article

Tran et al Nat Commun. 2021 Feb 19;12(1):1176. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-iLACCO1.1

Plasmid

#218822

Purpose

Mammalian expression of genetically encoded green fluorescent L-lactate biosensor with low affinity

Depositor

Robert Campbell

Article

Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

Insert

iLACCO1.1

Expression

Mammalian

Available Since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge