We narrowed to 53,191 results for: Ran

1. gfasPurple chromoprotein

   Plasmid

   #117849

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses gfasPurple chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   promoter, RBS, gfasPurple
   

   Use

   Synthetic Biology; Escherichia coli

   Tags

   Expression

   Mutation

   BioBrick sites removed

   Promoter

   Available since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPFO*

   Plasmid

   #120400

   Purpose

   Expresses Perfringolysin O cholesterol sensor, has N-terminal His6-tag and three point mutations, A71T, C459A, and Y181A. Y181A prevents PFO's lytic activity at 4°C.

   Depositor

   Arun Radhakrishnan

   Article

   Das et al Proc Natl Acad Sci U S A. 2013 Jun 25;11

   Insert

   Mutant form of Perfringolysin O
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   Amino acids 1-27 are deleted, starts at amino aci…

   Promoter

   Available since

   Jan. 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAS_4xMx PylT_GFP 150TAG

   Plasmid

   #140016

   Purpose

   Plasmid with 4xMxPylT cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   150TAG, K46E (Please see depositor comments below)

   Promoter

   EF1

   Available since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-CMV-mOC-STAMP-GFP

   Plasmid

   #73579

   Purpose

   Lentivirus expression of mOC-STAMP

   Depositor

   Paul Odgren

   Article

   Witwicka et al PLoS One. 2015 Jun 4;10(6):e0128275. doi

   Insert

   OC-STAMP (Ocstamp Mouse)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2016

   Availability

   Academic Institutions and Nonprofits only

5. GST-Rab8a-wt

   Plasmid

   #86078

   Purpose

   A bacterial expression plasmid encoding Rab8a wild type with N-terminus GST.

   Depositor

   Lei Lu

   Article

   Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

   Insert

   hRab8A (RAB8A Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.2/DEST/hTfR-HA

   Plasmid

   #69610

   Purpose

   human transferrin receptor with an HA tag fused at the C-terminus

   Depositor

   Robin Shaw

   Article

   Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

   Insert

   human transferrin receptor (TFRC Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNLF1W

   Plasmid

   #136404

   Purpose

   Expression of N-terminal NanoLuc luciferase fused proteins in mammalian cells. Genes can be cloned using Gateway cloning system. Cloned vectors can be used for NanoBRET assay.

   Depositor

   Sebastian Maurer

   Article

   Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   NanoLuc luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti-CMV-cGAS-N-HA

   Plasmid

   #130913

   Purpose

   Expresses human cGAS N terminus(aa1-159)-HA; Puromycin selection marker

   Depositor

   Jonathan Kagan

   Article

   Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

   Insert

   cGAS N terminus
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pNvT-MHC::mCh

   Plasmid

   #67943

   Purpose

   Transgenesis vector consisting of a 1.6-kb fragment of MyHC1 promoter region upstream of the start codon and an mCherry reporter gene, flanked by inverted binding sites of meganuclease I-SceI

   Depositor

   Ulrich Technau

   Article

   Renfer et al Proc Natl Acad Sci U S A. 2010 Jan 5;107

   Insert

   mCherry
   

   Use

   Transgenic reporter in the sea anemone, nematoste…

   Tags

   Expression

   Mutation

   Promoter

   Myosin Heavy Chain1 (MyHC1)

   Available since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTDH3-dCas9-Mxi1

    Plasmid

    #46921

    Purpose

    Yeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS and Mxi1 domain controlled by TDH3 promoter

    Depositor

    Stanley Qi, Jonathan Weissman

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Insert

    dCas9-Mxi1
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. siRNA-resistant ss-SigmaR1DTM-mNeonGreen R119A F191A

    Plasmid

    #226576

    Purpose

    Encodes mutant siRNA resistant SigmaR1 protein (transmembrane region deletion + substitutions) labeled with mNeonGreen

    Depositor

    Gia Voeltz

    Article

    Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

    Insert

    SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    R119A, F191A

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMuLE ENTR SV40 PuroR L3-L2

    Plasmid

    #62164

    Purpose

    MuLE (Multiple Lentiviral Expression) Entry vector containing a SV40 promoter and Puromycin module. Compatible with MultiSite Gateway cloning

    Depositor

    Ian Frew

    Article

    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

    Insert

    Puromycin
    

    Use

    Mule gateway entry vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. asPink chromoprotein

    Plasmid

    #117838

    Purpose

    BioBrick pSB1C3 plasmid that constitutively overexpresses asPink chromoprotein in E. coli

    Depositor

    Anthony Forster

    Article

    Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

    Insert

    promoter, RBS, asPink
    

    Use

    Synthetic Biology; Escherichia coli

    Tags

    Expression

    Mutation

    BioBrick sites removed

    Promoter

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHJ12: CadC-VHH-Caffeine (No linker)

    Plasmid

    #108244

    Purpose

    Membrane bound transcription activator CadC fusing VHH-Caffeine. Upon ligand binding, CadC-VHH-Caffeine can be dimerized and activate down stream reporter gene sfGFP expression

    Depositor

    Jerome Bonnet

    Article

    Chang et al ACS Synth Biol. 2017 Oct 30. doi: 10.102

    Insert

    Membrane bound transcription activator CadC fusing with VHH-Caffeine antibody without external linker
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    pLacO1

    Available since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. p(A)n-PAUSE

    Plasmid

    #118068

    Purpose

    Transcription Blocker - Synthetic polyadenylation signal & RNA polymerase II transcriptional pause signal from the human α2 globin gene

    Depositor

    Koen Venken

    Article

    Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

    Insert

    Synthetic polyadenylation signal & RNA polymerase II transcriptional pause signal from the human α2 globin gene
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDSP16 (5’Δ Cbr-unc-119(+) + loxN + MCS + 5’Δ mNeonGreen)

    Plasmid

    #193854

    Purpose

    Promoterless insertion vector for split Cbr-unc-19(+)/mNeonGreen landing pad

    Depositor

    Patrick Phillips

    Article

    Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

    Insert

    5' Cbr-unc-119(+) + 5' mNeonGreen
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Cbr-unc-119p

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. p2xFLAGhYAP1

    Plasmid

    #17791

    Depositor

    Marius Sudol

    Article

    Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

    Insert

    Yes-kinase associated protein (YAP1 Human)
    

    Use

    Tags

    2xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 9, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR-CMV-TetO2_3C-Avi-His6__IRES-EmGFP

    Plasmid

    #113888

    Purpose

    Mammalian (inducible) protein expression, in vivo biotinylation, bicistronic expression of cytosolic EmGFP

    Depositor

    A. Radu Aricescu

    Article

    Elegheert et al Nat Protoc. 2018 Nov 19. pii: 10.1038/s4

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3C-Avi-His6

    Expression

    Mutation

    Promoter

    CMV-MIE-TetO2

    Available since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTV-Pou5f1g5prime-1000-24MS96T-3F_NeoR

    Plasmid

    #174883

    Purpose

    Targeting vector for generating Pou5f1 STREAMING-tag KI cell

    Depositor

    Hiroshi Ochiai

    Article

    Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

    Insert

    STREAMING-tag with homology arms for Pou5f1
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMax_GFP_C289T

    Plasmid

    #177826

    Purpose

    Site-specific mutagenesis of GFP for the purposes of creating a site-specific hypoxanthine lesion for the purposes of measuring MPG activity via Host Cell Reactivation (HCR).

    Depositor

    Zachary Nagel, Leona Samson

    Article

    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

    Insert

    GFP_C289T
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    C289T

    Promoter

    CMV

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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