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pRQ-Id1HA

Plasmid

#18112

Depositor

Alex Swarbrick

Article

Swarbrick et al Proc Natl Acad Sci U S A. 2008 Apr 8. 10

Insert

Inhibitor of differentiation 1 (Id1 Mouse)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

Jan. 29, 2009

Availability

Academic Institutions and Nonprofits only

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pCMV5 Flag-Par6 delta 250-295 Flag tagged

Plasmid

#24646

Depositor

Jeff Wrana

Article

Narimatsu et al Cell. 2009 Apr 17. 137(2):295-307.

Insert

Par6c (Pard6a Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

Deletion of the PDZ domain of Par6c (aa 250-295)

Available Since

June 2, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pPU-Empty-miR30-TRIM5

Plasmid

#132936

Purpose

All-in-one control rescue-TRIM5 shRNA knockdown vector

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

control shRNA

Use

Lentiviral and RNAi

Tags

HA

Expression

Mammalian

Promoter

SFFV

Available Since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pMXs_FLAG-SFXN4

Plasmid

#110638

Purpose

Retroviral expression of Flag-SFXN4 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Science. 2018 Nov 16;362(6416). pii: 362

Insert

SFXN4 (SFXN4 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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KTK_136

Plasmid

#180520

Purpose

Empty backbone for D1.1 cloning, contains sfGFP dropout

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Type

Empty backbone

Expression

Bacterial

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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pITGFP89

Plasmid

#83560

Purpose

Used to evaluate the expression output of TEF1 promoter with yEGFP-CLN2PEST (destabilized GFP) used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

TEF1 promoter -yEGFP-CLN2PEST

Expression

Yeast

Promoter

TEF1

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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-962 human cyclin D1 promoter EtsA site mutant pGL3Basic

Plasmid

#32729

Depositor

Frank McCormick

Article

Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

Insert

CCND1 promoter (CCND1 Human)

Use

Luciferase; Luciferase reporter gene

Mutation

-822 EtsA site AGGAA deletion

Promoter

-962 CCND1 promoter

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only
-962 human cyclin D1 promoter EtsB site mutant pGL3Basic

Plasmid

#32730

Depositor

Frank McCormick

Article

Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

Insert

CCND1 (CCND1 Human)

Use

Luciferase

Mutation

-778 EtsB binding site AGGAA deletion

Promoter

-962 CCND1

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only
pSMP-MBD2_2

Plasmid

#36369

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

MBD2 (MBD2 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pUASP dsRed Rab11 CA

Plasmid

#44830

Depositor

Matthew Scott

Article

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab11 (Rab11 Fly)

Use

Drosophila

Tags

dsRed

Expression

Insect

Mutation

Q70L (constitutive active)

Available Since

April 18, 2013

Availability

Academic Institutions and Nonprofits only
NET33 pmRFP-N2 (448)

Plasmid

#61979

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Malik et al Cell Mol Life Sci. 2010 Apr;67(8):1353-6

Insert

NET33 (SCARA5 Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
MigR1-Flag-TRIB2

Plasmid

#131154

Purpose

Retroviral expression of flag-tagged mouse TRIB2

Depositor

Karen Keeshan, Warren Pear

Article

Keeshan et al Cancer Cell. 2006 Nov;10(5):401-11. doi:

Insert

Trib2 (Trib2 Mouse)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pUPD2 AtUBQ10 (GB2478)

Plasmid

#160594

Purpose

UBQ10 promoter from A. thaliana for constitutive expression in plant systems.

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

Insert

AtUBQ10

Use

CRISPR and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pJR2953

Plasmid

#122549

Purpose

Expression of a C. elegan codon optimized florescent protein (CemCardinal2) under the intestinal specific promoter nhx-2

Depositor

Gary Silverman

Article

Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do

Insert

Pnhx-2_CemCardinal2

Expression

Bacterial

Promoter

Pnhx-2

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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sspa-GCaMP6f

Plasmid

#67561

Purpose

short photoactivatable fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

sspa-GCaMP6f

Expression

Mammalian

Mutation

K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

Available Since

July 29, 2015

Availability

Academic Institutions and Nonprofits only
pSMP-MBD2_1

Plasmid

#36368

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

MBD2 (MBD2 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pET51b(+)-NADPH-LUCID-4 (C50 6.5 uM)

Plasmid

#117911

Purpose

Plasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 6.5 uM

Depositor

Kai Johnsson

Article

Yu et al Science. 2018 Sep 14;361(6407):1122-1126

Insert

SNAP_Pro30_[cpNLuc23_24]eDHFR(R44A-H45Q)

Tags

His-tag and Strep-tag

Expression

Bacterial

Mutation

R44A & H45Q on eDHFR

Promoter

T7

Available Since

Oct. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5-Myc-SAMTOR

Plasmid

#100517

Purpose

Expresses Myc tagged SAMTOR in mammalian cells

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

SAMTOR (BMT2 Human)

Tags

Myc

Expression

Mammalian

Available Since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only

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-962 human cyclin D1 promoter EtsC site mutant pGL3Basic

Plasmid

#32731

Depositor

Frank McCormick

Article

Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

Insert

CCND1 promoter (CCND1 Human)

Use

Luciferase

Mutation

-424 EtsC site TTCCT deletion

Promoter

-962 CCND1 promoter

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only
pCDH-EF1a-tetR-T2A-tSapphire

Plasmid

#176312

Purpose

construct expresses tet repressor (tetR) to reduce leakiness together with the fluorochrome t-Sapphire

Depositor

Irmela Jeremias

Article

Liu et al Biomark Res. 2020 Sep 16;8:46. doi: 10.1

Inserts

tetR
T-sapphire

Use

Lentiviral

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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