We narrowed to 11,278 results for: Vars;

1. CMV-mito-Citroff1-S245

   Plasmid

   #134306

   Purpose

   mitochondria-targeted inverse-response citrate sensor variant with medium binding affinity

   Depositor

   Robert Campbell

   Article

   Zhao et al ACS Cent Sci. 2020 Aug 26;6(8):1441-1450

   Insert

   Citroff1 S245A
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(+) mFis1 V1

   Plasmid

   #44600

   Depositor

   David Chan

   Article

   Loson et al Mol Biol Cell. 2013 Jan 2.

   Insert

   mFis1 variant 1 (Fis1 Mouse)
   

   Expression

   Mammalian

   Available Since

   April 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCFD2-dU6:2gRNA

   Plasmid

   #49409

   Purpose

   expression of gRNA under control of the Drosophila U6:2 promoter

   Depositor

   Simon Bullock

   Article

   Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

   Insert

   dU6-2:gRNA
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   dU6-2

   Available Since

   Jan. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJH645

   Plasmid

   #172169

   Purpose

   Reporter plasmid for pylBCD variant in pBAD backbone

   Depositor

   Matthew Bennett

   Article

   Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

   Insert

   Pem7 luxCDA(B.1TAG)E; Plpp pylS; PproK pylT
   

   Expression

   Bacterial

   Mutation

   Inserted amber codon immediately after initiator …

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCFD1-dU6:1gRNA

   Plasmid

   #49408

   Purpose

   expression of gRNA under control of the Drosophila U6:1 promoter

   Depositor

   Simon Bullock

   Article

   Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

   Insert

   dU6-1:gRNA
   

   Use

   CRISPR

   Expression

   Insect

   Promoter

   dU6-1

   Available Since

   Jan. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-sCAG-eGFP-RAB11

   Plasmid

   #203799

   Purpose

   AAV vector plasmid expressing human RAB11 fused to eGFP under a short variant of the CAG (sCAG) promoter

   Depositor

   Bart Nieuwenhuis, Richard Eva

   Insert

   RAB11 (RAB11A Human)
   

   Use

   AAV

   Tags

   eGFP

   Expression

   Mammalian

   Available Since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET21b-MBPx-MBPx-SpyTag003

   Plasmid

   #216291

   Purpose

   Expresses tandem MBPx linked to-SpyTag003 in bacterial cells. MBPx is a variant of MBP (A312V I317V Δ172-173 Δ175-176) with enhanced affinity for maltose.

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   MBPx-MBPx-SpyTag003
   

   Use

   Affinity Reagent/ Antibody

   Tags

   His6 and SpyTag003

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. U6-(BbsI)sgRNA_CAG-Venus-bpA

   Plasmid

   #86985

   Purpose

   For cloning and expression of sgRNA together with Venus expression

   Depositor

   Ralf Kuehn

   Article

   Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

   Insert

   Venus
   

   Expression

   Mammalian

   Mutation

   Venus GFP variant

   Promoter

   CAG

   Available Since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBac{3XP3::DsRed, Pactin::Ptrsps}

   Plasmid

   #86862

   Purpose

   Expresses DsRed in eyes and ubiquitous expression of P-transposase

   Depositor

   David Stern

   Article

   Stern et al G3 (Bethesda). 2017 Apr 3;7(4):1339-1347

   Insert

   pACNNTNPII-S129A
   

   Expression

   Insect

   Mutation

   S129A

   Available Since

   June 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. scFv-IFP2.0N

    Plasmid

    #215746

    Purpose

    Expresses single chain Fragment variable (scFv) fused to N-terminal part of split IFP2.0

    Depositor

    Albert Jeltsch

    Article

    Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

    Insert

    IFP2.0N
    

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. P305-FBP_4

    Plasmid

    #139695

    Purpose

    T-DNA encoding Fungal Bioluminescent Pathway Components, complete pathway

    Depositor

    Daniel Voytas

    Article

    Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

    Insert

    pLHB1B1:NnCPH:tSlH4, p35S_Short_TMV 5':AsNpga:tAtug7, pCmYLCV:NnH3H:tAtuOCS, pCmYLCV:NnHisps:tHsp, pAtuMas:NnLuz:trbcSE9
    

    Use

    Luciferase

    Expression

    Plant

    Promoter

    various

    Available Since

    May 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDL17_Cas9-His Δcys M1C

    Plasmid

    #206251

    Purpose

    Bacterial expression of SpCas9 Δcys M1C variant under T7 promoter

    Depositor

    Amit Choudhary

    Article

    Lim et al Nat Commun. 2020 Aug 13;11(1):4043. doi:

    Insert

    SpCas9
    

    Use

    CRISPR

    Tags

    6x-His and SV40 NLS

    Expression

    Bacterial

    Mutation

    M1C, C80S, C574S

    Promoter

    T7

    Available Since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTEV5_ RpMatB_1

    Plasmid

    #78205

    Purpose

    Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor

    Depositor

    Martin Webb

    Article

    Vancraenenbroeck et al ACS Chem Biol. 2015 Nov 20;10(11):2650-7

    Insert

    matB (His6 C106A R286C Q457C K488A)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    C106A R286C Q457C K488A

    Promoter

    T7

    Available Since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pCMV-AAAAA-nCas9(D10A)-UGI

    Plasmid

    #187409

    Purpose

    DddA AAAAA variant

    Depositor

    Jin-Soo Kim

    Article

    Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

    Insert

    DddA AAAAA
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLP-pR-himar-apraR

    Plasmid

    #199996

    Purpose

    Landing Pad with T7 RNAP circuit variant T15. pC194+Tn903 seed promoter. pR-himar transposase. In dap auxotrophic conjugation donor

    Depositor

    Farren Isaacs

    Article

    Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

    Insert

    Landing Pads
    

    Expression

    Bacterial

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. 1/8-NORAD-(A)4x

    Plasmid

    #199209

    Purpose

    1/8 truncation of NORAD mRNA tagged with 4 copies of Riboglow (variant A)

    Depositor

    Esther Braselmann

    Article

    Sarfraz et al Nat Commun. 2023 Feb 16;14(1):867. doi:

    Insert

    1/8-NORAD
    

    Tags

    Riboglow RNA tag

    Expression

    Mammalian

    Mutation

    4 copies of Riboglow tag after stop codon

    Promoter

    CMV

    Available Since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. 1/2-NORAD-(A)4x

    Plasmid

    #199208

    Purpose

    1/2 truncation of NORAD mRNA tagged with 4 copies of Riboglow (variant A)

    Depositor

    Esther Braselmann

    Article

    Sarfraz et al Nat Commun. 2023 Feb 16;14(1):867. doi:

    Insert

    1/2-NORAD
    

    Tags

    Riboglow RNA tag

    Expression

    Mammalian

    Mutation

    4 copies of Riboglow tag after stop codon

    Promoter

    CMV

    Available Since

    June 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. UAS:Zebrabow-V

    Plasmid

    #118218

    Purpose

    For broad or variegated gal4 inducible expression of Brainbow in zebrafish

    Depositor

    Albert Pan, Alex Schier

    Article

    Pan et al Development. 2013 Jul;140(13):2835-46. d

    Insert

    Brainbow 1.0 L
    

    Use

    Zebrafish expression

    Available Since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBac{3XP3::EGFP, Pactin::Ptrsps}

    Plasmid

    #86861

    Purpose

    Expresses EGFP in eyes and ubiquitous expression of P-transposase

    Depositor

    David Stern

    Article

    Stern et al G3 (Bethesda). 2017 Apr 3;7(4):1339-1347

    Insert

    pACNNTNPII-S129A
    

    Expression

    Insect

    Available Since

    June 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. px458 EQR

    Plasmid

    #101731

    Purpose

    Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 EQR
    

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    EQR (D1135E, R1335Q, and T1337R mutations in Cas9)

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. pcDNA3.1_hMed25

    Plasmid

    #206958

    Purpose

    The ORF of human Med25 cDNA amplified from the RT human liver RNA was cloned into pGEMTE vector and then subclone at XhoI/XbaI sites of the pcDNA3.2(+), the mammalian expression vector.

    Depositor

    Catharine Ross, Reza Zolfaghari

    Insert

    Homo sapiens mediator complex subunit 25 (MED25), transcript variant 1, mRNA (MED25 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. pARR5::LUC

    Plasmid

    #141170

    Purpose

    Luminescent reporter for cytokinin signaling

    Depositor

    Patrick Schäfer, Vardis Ntoukakis

    Article

    Lehmann et al PLoS One. 2020 Jun 4;15(6):e0234154. doi

    Insert

    AtARR5 promoter
    

    Use

    Luciferase

    Expression

    Plant

    Promoter

    AtARR5, AT3G48100

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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23. CAG:: ChR2HA-2a-hM4D

    Plasmid

    #52520

    Purpose

    Co-expresses HA tagged channelrhodopsin and hM4D (Gi)

    Depositor

    Scott Sternson

    Article

    Stachniak et al Neuron. 2014 May 21;82(4):797-808. doi:

    Insert

    ChR2-2a-hM4D
    

    Tags

    2xHA on ChR2

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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24. pGP-RSET-iGABASnFR2n-6xHis

    Plasmid

    #218886

    Purpose

    Bacterial expression of improved GABA sensor (negative change in fluorescence)

    Depositor

    GENIE Project

    Article

    Kolb et al bioRxiv 2025.03.25.644953

    Insert

    iGABASnFR2n
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    S99A F104H R168P

    Promoter

    T7

    Available Since

    May 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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25. pRAB18::LUC

    Plasmid

    #141168

    Purpose

    Luminescent reporter for abscisic acid signaling

    Depositor

    Patrick Schäfer, Vardis Ntoukakis

    Article

    Lehmann et al PLoS One. 2020 Jun 4;15(6):e0234154. doi

    Insert

    AtRAB18 promoter
    

    Use

    Luciferase

    Expression

    Plant

    Promoter

    AtRAB18, AT5G66400

    Available Since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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26. pET30 GLTP

    Plasmid

    #170733

    Purpose

    The plasmids contain inserts (ORFs) for expressing various members of the GlycoLipid Transfer Protein (GLTP) superfamily.

    Depositor

    Rhoderick Brown

    Article

    Malinina et al Nature. 2004 Aug 26;430(7003):1048-53. d

    Insert

    GLTP (GLTP Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    July 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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27. pYC34

    Plasmid

    #63915

    Purpose

    Recipient overexpressing vector to construct mCherry tagged fusions

    Depositor

    Irene Castano Navarro

    Article

    Yanez-Carrillo et al Fungal Genet Biol. 2015 May 15. pii: S10

    Type

    Empty backbone

    Tags

    mCherry

    Expression

    Yeast

    Promoter

    TEF1 (Saccharomyces cerevisiae)

    Available Since

    April 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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28. px330 VRER

    Plasmid

    #101729

    Purpose

    Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VRER
    

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VRER (D1135V, G1218R, R1335E, and T1337R mutation…

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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29. AAV-hRedOpsin-TGFB2

    Plasmid

    #164428

    Purpose

    AAV plasmid expressing TGFB2 in photoreceptors

    Depositor

    Connie Cepko

    Article

    Wang et al J Clin Invest. 2020 Aug 3;130(8):4360-43

    Insert

    Tgfb2 (Tgfb2 Mouse)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    human red opsin

    Available Since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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30. pKar2-msGFP-HDEL

    Plasmid

    #117668

    Purpose

    Localisation of Endoplasmic Reticulum

    Depositor

    Benjamin Glick, Pau Ferrer

    Article

    Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

    Insert

    pKar2-msGFP-HDEL
    

    Expression

    Yeast

    Mutation

    Contains the Kar2 promoter and signal sequence fr…

    Promoter

    pKar2

    Available Since

    March 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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