165,528 results

1. KARS_pNIC-Bio3

   Plasmid

   #153051

   Purpose

   Expression construct for producing Avi tagged Lysyl-tRNA synthetase

   Depositor

   Susanne Gräslund

   Insert

   KARS (KARS1 Human)
   

   Tags

   Avi and His6-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGs-NLS-TIR1_P2A_NES-TIR1

   Plasmid

   #117699

   Purpose

   Expression of NLS-(Os)TIR1-HA and Myc-NES-(Os)TIR1from one mRNA

   Depositor

   Joerg Mansfeld

   Article

   Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

   Inserts

   TRANSPORT INHIBITOR RESPONSE 1
   TRANSPORT INHIBITOR RESPONSE 1
   

   Tags

   HA tag, Myc-NES, and NLS

   Expression

   Mammalian

   Mutation

   codon optimized for murine expression

   Promoter

   None, 2A peptide from porcine teschovirus-1 polyp…

   Available Since

   Jan. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. IRES-mCherry

   Plasmid

   #80139

   Purpose

   empty retroviral vector with IRES-mCherry

   Depositor

   Ellen Rothenberg

   Article

   Kueh et al Science. 2013 Aug 9;341(6146):670-3. doi

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   IRES-mCherry

   Expression

   Mammalian

   Available Since

   July 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDRM210 LGP (Luciferase GFP Puro)

   Plasmid

   #174722

   Purpose

   Expresses the firefly luciferase gene, E2A, eGFP, F2A, and the puromycin resistance gene

   Depositor

   Wayne Tilley

   Article

   Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413

   Inserts

   Firefly Luciferase
   eGFP
   pac
   

   Use

   Lentiviral and Luciferase

   Tags

   E2A linker and F2A linker

   Expression

   Mammalian

   Promoter

   MND, MND (off Luc-E2A), and MND (off Luc-E2A-eGFP…

   Available Since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOpen_taq

   Plasmid

   #153315

   Purpose

   Bacterial expression of Taq polymerase

   Depositor

   Drew Endy

   Insert

   Taq polymerase
   

   Expression

   Bacterial

   Promoter

   Tac

   Available Since

   June 2, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pEGFP-Akt1(WT)

   Plasmid

   #86637

   Purpose

   Expresses eGFP-tagged human Akt1(WT)

   Depositor

   Ivan Yudushkin, Thomas Leonard

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt1 (AKT1 Human)
   

   Expression

   Mammalian

   Available Since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPB-CAG-hCas3

   Plasmid

   #134920

   Purpose

   Components for genome editing in mammalian cells with pCAG-All-in-one-hCascade and pBS-U6-crRNA-targeted.

   Depositor

   Tomoji Mashimo

   Article

   Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:

   Insert

   Cas3 with bpNLS
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAK_lentiguide_DR30_Puro

   Plasmid

   #134839

   Purpose

   Lentiviral expression CasRx guideRNA

   Depositor

   Michael Yaffe

   Insert

   CasRx guideRNA backbone
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. SpCas9-CBE6b-V106W

   Plasmid

   #215825

   Purpose

   Express CBE6b V106W (with SpCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

   Insert

   CBE6b V106W-SpCas9-2xUGI (cas9 )
   

   Use

   In vitro transcription template

   Available Since

   March 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. Galt1-Grx1-roGFP1-iE

    Plasmid

    #230029

    Purpose

    Expressing Grx1-roGFP1-iE fluorescent fusion protein in mammalian cells with localization in the Golgi apparatus.

    Depositor

    Pablo Rivera-Fuentes

    Insert

    Galt1-Grx1-roGFP1-iE (B4GALT1 Human, Synthetic)
    

    Expression

    Mammalian

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-CXCL12-sfGFP

    Plasmid

    #98961

    Purpose

    Mammalian expression plasmid for superfolder GFP-fused human chemokine CXCL12

    Depositor

    Erik Procko

    Article

    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

    Insert

    CXCL12 (CXCL12 Human)
    

    Tags

    Superfolder GFP

    Expression

    Mammalian

    Mutation

    Full-length, wildtype

    Promoter

    CMV

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTP1183

    Plasmid

    #104163

    Purpose

    Bacterial expression plasmid of anti-rabbit IgG Fc nanobody TP897 (1x Cysteine)

    Depositor

    Dirk Görlich

    Article

    Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

    Insert

    Anti-rabbit IgG Fc specific nanobody TP897 (1xCysteine)
    

    Tags

    14xHistidine tag and NEDD8 from Brachypodium dist…

    Expression

    Bacterial

    Available Since

    Dec. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDH-EF1-copGFP-T2A-Puro

    Plasmid

    #72263

    Purpose

    Express copGFP and puromycin resistance gene under EF1 promoter

    Depositor

    Kazuhiro Oka

    Insert

    copGFP-T2A-Puro
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    elongation factor-1

    Available Since

    Jan. 11, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pAC-GAL4

    Plasmid

    #24344

    Depositor

    Liqun Luo

    Article

    Potter et al Cell. 2010 Apr 30. 141(3):536-48.

    Insert

    GAL4
    

    Expression

    Insect

    Available Since

    March 31, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-opCas12a-6xNLS

    Plasmid

    #209022

    Purpose

    Lentiviral vector expressing human codon-optimized OpCas12a with 6xNLS

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    opCas12a-6xNLS-Flag-Myc-NeoR
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG and MYC

    Expression

    Mammalian

    Mutation

    E174R/S542R

    Promoter

    EF-1a

    Available Since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA4 HisMax-V5-GFP-RRBP1

    Plasmid

    #92150

    Purpose

    Expresses V5- and EGFP-tagged human RRBP1 (with 54 decapeptide repeats) in mammalian cells.

    Depositor

    Alice Ting

    Article

    Hung et al Elife. 2017 Apr 25;6. pii: e24463. doi:

    Insert

    RRBP1 (RRBP1 Human)
    

    Tags

    Calreticulin signal peptide, EGFP (A207K mutation…

    Expression

    Mammalian

    Mutation

    In RRBP1: N951S and T1329L. E1279 is also missing.

    Promoter

    CMV

    Available Since

    Aug. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. MSCV-IRES-Thy1.1 DEST

    Plasmid

    #17442

    Purpose

    Destination vector derived from MSCV-IRES-Thy1.1. Please note that this plasmid contains attB1 and attB2 sites for use in a BP reaction

    Depositor

    Anjana Rao

    Article

    Wu et al Cell. 2006 Jul 28. 126(2):375-87.

    Type

    Empty backbone

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Feb. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE

    Plasmid

    #32702

    Purpose

    Conditional overexpression vector. Deletion of dsRed by Cre recombinase results in the rapid loss of dsRed and the activation of your gene fused to eGFP expression.

    Depositor

    Hans Clevers

    Article

    Koo et al Nat Methods. 2011 Dec 4. doi: 10.1038/nm

    Insert

    eGFP
    

    Use

    Cre/Lox and Retroviral

    Expression

    Mammalian

    Available Since

    Dec. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBS-L1PA1-CH-mneo

    Plasmid

    #51288

    Purpose

    contains the fully codon optimized ORF1 and ORF2 of the human L1RP

    Depositor

    Astrid Roy-Engel

    Article

    Wagstaff et al PLoS One. 2011 May 10;6(5):e19672. doi:

    Insert

    L1 (L1RE1 Human)
    

    Tags

    mneoI indicator cassette containing an inverted n…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEThT-RvLEAM

    Plasmid

    #90033

    Purpose

    To express His-tagged RvLEAM in bacteria

    Depositor

    Takekazu Kunieda

    Article

    Tanaka et al PLoS One. 2015 Feb 12;10(2):e0118272. eC

    Insert

    RvLEAM
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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