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EF1a_POU5F1_P2A_Hygro_Barcode

Plasmid

#120474

Purpose

Barcoded lentiviral vector to express POU5F1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Publication

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

POU5F1 (POU5F1 Human)

Use

Lentiviral

Promoter

EF1a

Available since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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Ad-EphA3 KD

Plasmid

#102726

Purpose

Expression of Kinase dead EphA3

Depositor

Jin Chen

Publication

Frieden et al Dev Dyn. 2010 Dec;239(12):3226-34. doi:

Insert

EphA3 (Epha3 Mouse)

Use

Adenoviral

Mutation

Kinase Dead (K657M; AAA > ATG)

Available since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pLEX307-mFzd7

Plasmid

#102869

Purpose

Expresses mouse Fzd7 in mammalian cells

Depositor

Henry Ho

Publication

Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

Insert

Frizzled 7 (Fzd7 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1A

Available since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pLEX307-mFzd1

Plasmid

#102864

Purpose

Expresses mouse Fzd1 in mammalian cells

Depositor

Henry Ho

Publication

Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

Insert

Frizzled 1 (Fzd1 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1A

Available since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLEX307-mFzd6

Plasmid

#102868

Purpose

Expresses mouse Fzd6 in mammalian cells

Depositor

Henry Ho

Publication

Susman et al Elife. 2017 Sep 8;6. pii: e26509. doi: 1

Insert

Frizzled 6 (Fzd6 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1A

Available since

Dec. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR221-H1-sgHTT1-U6-sgGFP2-7SK-sgCas9

Plasmid

#87917

Purpose

Gateway cloning

Depositor

Nicole Déglon

Publication

Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sgHTT1, sgGFP2, shCas9

Expression

Mammalian

Promoter

H1, U6, 7SK

Available since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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COPE_pLX307

Plasmid

#98327

Purpose

Lentiviral expression of COPE

Depositor

William Hahn, Sefi Rosenbluh

Publication

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

COPE (COPE Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

E1Fa

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pH3FS-GAL1

Plasmid

#85782

Purpose

N-ICE plasmid pH3FS-GAL1

Depositor

Scott Briggs

Publication

Zhang et al Yeast. 2016 Dec 12. doi: 10.1002/yea.322

Insert

loxP-HphMX-loxP-GAL1p-3FLAG

Use

Cre/Lox

Expression

Yeast

Available since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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LAMP-ReNL_pcDNA3

Plasmid

#89532

Purpose

Red color variant of bright luminescent protein, fused to Lysosomal-associated membrane protein 1

Depositor

Takeharu Nagai

Publication

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ReNL

Tags

LAMP1

Expression

Mammalian

Promoter

CMV

Available since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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Lyn-OeNL_pcDNA3

Plasmid

#89528

Purpose

Orange color variant of bright luminescent protein, targeted to the plasma membrane

Depositor

Takeharu Nagai

Publication

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

OeNL

Tags

Lyn

Expression

Mammalian

Promoter

CMV

Available since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGEMT-T-HSV2.3 TCR alpha chain

Plasmid

#74076

Purpose

gBT-I TCR a chain cDNA in pGEM-T

Depositor

Francis R. Carbone

Publication

Mueller et al Immunol Cell Biol. 2002 Apr;80(2):156-63

Insert

TCR alpha clone HSV2.3 (Tcra Mouse)

Expression

Bacterial

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

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pBabe-ER-E2F2-dRb

Plasmid

#62805

Purpose

expresses an inducible E2F2 mutant that lacks the Rb binding domain

Depositor

Gerardo Ferbeyre

Publication

Vernier et al Genes Dev. 2011 Jan 1;25(1):41-50. doi:

Insert

E2F2 (E2F2 Human)

Use

Retroviral

Tags

HA-Er

Expression

Mammalian

Mutation

deletion of amino acids 302-437, including the Rb…

Available since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Talin-18

Plasmid

#55447

Purpose

Localization: Focal Adhesions, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Publication

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Talin

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only

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mCerulean3-Plastin-C-10

Plasmid

#55438

Purpose

Localization: Fimbrin/Cytoskeleton, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Publication

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Plastin (PLS1 Human)

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pMXs-hs-CDC34

Plasmid

#52731

Purpose

retroviral expression of human CDC34

Depositor

Shinya Yamanaka

Publication

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

CDC34 (CDC34 Human)

Use

Retroviral

Expression

Mammalian

Available since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-HA-ERK5 K84R

Plasmid

#53171

Purpose

Expresses GST-HA tagged human ERK5 K84R from bacterial expression vector

Depositor

Christopher Counter

Publication

Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

Insert

ERK5 (MAPK7 Human)

Tags

GST and HA

Expression

Bacterial

Mutation

K84R

Available since

June 9, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
GFP-Rab10

Plasmid

#31737

Depositor

Ron Vale

Publication

Huckaba et al J Biol Chem. 2011 Mar 4;286(9):7457-67.

Insert

Rab10 (Rab10 Fly)

Tags

GFP

Expression

Insect

Promoter

Actin

Available since

Sept. 21, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
PET-eSpCas9-NLS-6xHis

Plasmid

#207379

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Publication

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

eSpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

K848A, K1003A, R1060A

Promoter

T7

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pRU51

Plasmid

#167677

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Publication

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

PcUB4-2::spCas9

Use

CRISPR

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pRU52

Plasmid

#167678

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Publication

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

PcUB4-2::spCas9

Use

CRISPR

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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