We narrowed to 13,466 results for: cDNA

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pcDNA5 rtTA BirA eIF4G +MIC

Plasmid

#158790

Purpose

pcDNA5 based transfection plasmid for the exogenous expression of N-terminal BirA-Flag-tagged eIF4G1 including the microexon for generation of N2A FlpIn cell lines for BioID

Depositor

Benjamin Blencowe

Article

Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

Insert

eIF4G1 (with microexon)

Tags

3xFlag and BirA*

Expression

Mammalian

Promoter

miniCMV

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3/flag-pBirA(1-K283)-FRB

Plasmid

#154451

Purpose

expresses flag-pBirA(1-K283)-FRB/biochemical assay

Depositor

Hyun-Woo Rhee

Article

Kwak et al Proc Natl Acad Sci U S A. 2020 May 15. p

Insert

flag-pBirA(1-K283)-FRB

Expression

Mammalian

Promoter

T7 promoter

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Venus-cpVenus-FLARE-EKAR-EV(T/A)

Plasmid

#123340

Purpose

Negative control mutant for Venus-cpVenus-FLARE-EKAR-EV biosensor.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

Venus-cpVenus-FLARE-EKAR-EV(T/A)

Tags

6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

Expression

Mammalian

Mutation

Target Thr residue in substrate domain mutated to…

Promoter

CMV

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA8-Flag-LARS (361-720aa)

Plasmid

#139688

Purpose

Expresses N-teminal Flag tagged aa 361-720 of LARS1

Depositor

Jie Chen

Article

Yoon et al Cell Rep. 2016 Aug 9;16(6):1510-1517. do

Insert

LARS1 (LARS1 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mCherry-mCherry-FLARE-CKAR

Plasmid

#123345

Purpose

Red-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase C activity.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

mCherry-mCherry-FLARE-CKAR

Tags

6xHIS, T7 tag (gene 10 leader), Xpress (TM) tag, …

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Venus-cpVenus-FLARE-CKAR(T/A)

Plasmid

#123344

Purpose

Negative control mutant for Venus-cpVenus-FLARE-CKAR biosensor.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

Venus-cpVenus-FLARE-CKAR(T/A)

Tags

6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

Expression

Mammalian

Mutation

Target Thr residue in substrate domain mutated to…

Promoter

CMV

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Cerulean3-cpCerulean3-FLARE-AKAR

Plasmid

#123336

Purpose

Cyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity. Contains monomeric Cerulean3/cpCerulean3-E173 homoFRET pair.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

Cerulean3-cpCerulean3-FLARE-AKAR

Tags

6xHIS, Cerulean3, T7 tag (gene 10 leader), Xpress…

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Cerulean3-Cerulean3-FLARE-AKAR

Plasmid

#123335

Purpose

Cyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

Cerulean3-Cerulean3-FLARE-AKAR

Tags

6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT-TO-EGFP-UPF1-R615A

Plasmid

#136001

Purpose

DOX-inducible R615A UPF1 inserted with GFP tagged on the N-terminus and used with the Flp-In T-Rex System

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

UPF1

Tags

EGFP

Expression

Mammalian

Mutation

R615A

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT-TO-EGFP-UPF1-DEAA

Plasmid

#136002

Purpose

DOX-inducible DEAA UPF1 inserted with GFP tagged on the N-terminus and used with the Flp-In T-Rex System

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

UPF1

Tags

EGFP

Expression

Mammalian

Mutation

DE647AA

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4/TO-ORF66 200-429-2xStrep

Plasmid

#130955

Purpose

Expresses KSHV ORF66 a.a. 200-429 with a C-terminal 2xStrep tag in mammalian cells

Depositor

Britt Glaunsinger

Article

Didychuk et al J Virol. 2019 Oct 2. pii: JVI.01299-19.

Insert

ORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))

Tags

2xStrep

Expression

Mammalian

Mutation

ORF66 a.a. 200-429

Promoter

CMV

Available Since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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hsa-miR-199-dendra2 PCDNA

Plasmid

#121133

Purpose

mammalian cell expression

Depositor

Qing Deng

Article

Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

Insert

hsa-miR-199

Expression

Bacterial

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/CUG-stop-nLuc-3XFLAG

Plasmid

#127309

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from CUG start codon, but translation terminated immediately by stop codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/NDUFS2 _34-66/myc-His

Plasmid

#127495

Purpose

Expresses myc-tagged _34-66 NDUFS2 in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS2 _34-66 (NDUFS2 Human)

Tags

myc-His

Expression

Mammalian

Mutation

NDUFS2 _34-66

Promoter

CMV

Available Since

July 16, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/NDUFS2 E57A/myc-His

Plasmid

#127497

Purpose

Expresses myc-tagged NDUFS2/E57A in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS2 E57A (NDUFS2 Human)

Tags

myc-His

Expression

Mammalian

Mutation

NDUFS2 E57A

Promoter

CMV

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/NDUFS2 T58A/myc-His

Plasmid

#127498

Purpose

Expresses myc-tagged NDUFS2/T58A in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS2 T58A (NDUFS2 Human)

Tags

myc-His

Expression

Mammalian

Mutation

NDUFS2 T58A

Promoter

CMV

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/NDUFS2 H60A/myc-His

Plasmid

#127499

Purpose

Expresses myc-tagged NDUFS2/H60A in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS2 H60A (NDUFS2 Human)

Tags

myc-His

Expression

Mammalian

Mutation

NDUFS2 H60A

Promoter

CMV

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/NDUFS2 W61A/myc-His

Plasmid

#127500

Purpose

Expresses myc-tagged NDUFS2/W61A in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS2 W61A (NDUFS2 Human)

Tags

myc-His

Expression

Mammalian

Mutation

NDUFS2 W61A

Promoter

CMV

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/NDUFS2 K62A/myc-His

Plasmid

#127501

Purpose

Expresses myc-tagged NDUFS2/K62A in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS2 K62A (NDUFS2 Human)

Tags

myc-His

Expression

Mammalian

Mutation

NDUFS2 K62A

Promoter

CMV

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/NDUFS2 P63A/myc-His

Plasmid

#127502

Purpose

Expresses myc-tagged NDUFS2/P63A in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS2 P63A (NDUFS2 Human)

Tags

myc-His

Expression

Mammalian

Mutation

NDUFS2 P63A

Promoter

CMV

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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