We narrowed to 15,723 results for: LEA

1. pCS2+ oDam_f_GFPoNLS

   Plasmid

   #85819

   Purpose

   iDamID plasmid. To express transiently the optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

   Depositor

   Joachim Wittbrodt

   Article

   Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

   Insert

   oDam_f_GFPoNLS
   

   Tags

   oNLS (optimized nuclear localization signal) C te…

   Expression

   Bacterial, Insect, Mammalia…

   Mutation

   L122A. Codon optimization compatible to work in M…

   Available Since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGBT9-Hook2

   Plasmid

   #198524

   Purpose

   Expression of GAL4 DNA-binding domain (BD)-Hook2 fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

   Insert

   Hook2 (HOOK2 Human)
   

   Tags

   GAL4-DNA binding domain fragment

   Expression

   Yeast

   Mutation

   His488Gln substitution

   Promoter

   ADH1

   Available Since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. sTR056

   Plasmid

   #177369

   Purpose

   Toehold switch, with an unpaired toehold region at the 5′-end that interacts with the trigger RNA (sTR060, Addgene plasmid # 177370) to unfold the switch.

   Depositor

   Jean-Loup Faulon, Jerome Bonnet

   Article

   Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

   Insert

   sfGFP
   

   Use

   Synthetic Biology; Cell-free protein synthesis

   Available Since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. sTR060

   Plasmid

   #177370

   Purpose

   Trigger RNA (GCAGGGATAAACGAGATAGATAAGATAAGA) that pairs with the toehold region in sTR056 (Addgene plasmid #177369) to restore translational activity.

   Depositor

   Jean-Loup Faulon, Jerome Bonnet

   Article

   Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

   Insert

   Trigger RNA
   

   Use

   Synthetic Biology; Cell-free protein synthesis

   Available Since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. p3xFLAG-MKL1 D301-380

   Plasmid

   #19852

   Depositor

   Ron Prywes

   Article

   Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

   Insert

   MKL1 (MRTFA Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   deleted amino acids 301 to 380

   Available Since

   Dec. 16, 2008

   Availability

   Academic Institutions and Nonprofits only

6. pEGFP-YOD1-MutS2

   Plasmid

   #85667

   Purpose

   Expression of human YOD1 I292Q V295Q mutant with N-terminal GFP tag

   Depositor

   Hemmo Meyer

   Article

   Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

   Insert

   YOD1 (YOD1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   I292Q and V295Q

   Promoter

   CMV

   Available Since

   April 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV-EF1a-V5-Emerin

   Plasmid

   #120246

   Purpose

   Expresses V5-tagged Emerin in lentiviral vector

   Depositor

   Larry Gerace

   Article

   Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

   Insert

   Emerin (Emd Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Promoter

   EF1a

   Available Since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. HOXB1 (human) HIS-tag pET

   Plasmid

   #8520

   Depositor

   Corey Largman

   Article

   Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

   Insert

   HOXB1 (HOXB1 Human)
   

   Tags

   His and T7

   Expression

   Bacterial

   Mutation

   PCR from cDNA clone

   Available Since

   July 6, 2006

   Availability

   Academic Institutions and Nonprofits only

9. pBabe-ER-E2F2-dRb

   Plasmid

   #62805

   Purpose

   expresses an inducible E2F2 mutant that lacks the Rb binding domain

   Depositor

   Gerardo Ferbeyre

   Article

   Vernier et al Genes Dev. 2011 Jan 1;25(1):41-50. doi:

   Insert

   E2F2 (E2F2 Human)
   

   Use

   Retroviral

   Tags

   HA-Er

   Expression

   Mammalian

   Mutation

   deletion of amino acids 302-437, including the Rb…

   Available Since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. lenti-sgRNA(MS2)_zeo_hPDX1_promoter_guide1

    Plasmid

    #176838

    Purpose

    To induce human PDX1 expression by recruiting SAM complex to PDX1 promoter

    Depositor

    Rudolf Jaenisch

    Article

    Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

    Insert

    sgPDX1
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSN69

    Plasmid

    #100125

    Purpose

    expresses putative cMYC DBD with a HA tag

    Depositor

    Mark Ptashne

    Article

    Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

    Insert

    mCerulean-P2A-putative cMYC DBD with a HA tag (MYC Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Insert corresponds to the DBD of cMYC

    Available Since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA4-hB90

    Plasmid

    #18968

    Depositor

    Mariano Viapiano

    Article

    Hu et al J Biol Chem. 2008 Sep 5;283(36):24848-59

    Insert

    Brevican C-terminus (BCAN Human)
    

    Expression

    Mammalian

    Mutation

    Human brevican signal peptide aa 1-23 followed by…

    Available Since

    Dec. 12, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pClneoMyc MOB1 T12A

    Plasmid

    #47936

    Purpose

    expresses Myc tagged human MOB1 containing T12A mutation

    Depositor

    Yutaka Hata

    Article

    Bao et al Genes Cells. 2009 Dec;14(12):1369-81. do

    Insert

    MOB1A (MOB1A Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    T12A

    Promoter

    CMV

    Available Since

    Sept. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3-HA-DNM2-W525L

    Plasmid

    #155263

    Purpose

    Mammalian expression of rat DNM2 fused to HA

    Depositor

    David Rubinsztein

    Article

    Puri et al Dev Cell. 2020 Apr 20;53(2):154-168.e6.

    Insert

    dynamin 2 (Dnm2 Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Tryptophan 525 to Leucine

    Available Since

    June 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFLAG-4S Tetherin

    Plasmid

    #41073

    Depositor

    Paul Spearman

    Article

    Hammonds et al J Virol. 2012 Feb;86(4):2259-72. Epub 20

    Insert

    Tetherin (BST2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S74, S84, 137S, and 144S

    Promoter

    CMV

    Available Since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

16. p2Flag CMV2-ZO-2-N

    Plasmid

    #27418

    Depositor

    Marius Sudol

    Article

    Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

    Insert

    Zonula Occludens 2 (TJP2 Human)
    

    Tags

    2xFlag

    Expression

    Mammalian

    Mutation

    Deletion of the carboxy-terminus of ZO-2, so only…

    Available Since

    April 12, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pX330-NQL007-NANOG-sgRNA

    Plasmid

    #175555

    Purpose

    For transient expression of spCas9-nuclease and a sgRNA targeting the mouse NANOG locus.

    Depositor

    Elzo de Wit

    Article

    Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

    Insert

    spCas9-nuclease and sgRNA against mouse NANOG STOP Codon
    

    Use

    Mouse Targeting

    Expression

    Mammalian

    Available Since

    Nov. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVX-EF1a-EGFP-TA Bik-IRES-Puromycin

    Plasmid

    #133026

    Purpose

    Expresses EGFP-tagged tail anchor sequence of Bik

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Bcl-2-interacting killer (BIK Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-110

    Promoter

    Human elongation factor 1 alpha

    Available Since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMKO.1-shEsco2

    Plasmid

    #160957

    Purpose

    Esco2 shRNA in pMKO.1 retroviral vector

    Depositor

    Matthew Steinhauser

    Article

    Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

    Insert

    Esco2 shRNA (Esco2 Mouse)
    

    Use

    RNAi and Retroviral

    Promoter

    U6

    Available Since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFLAG-137.144S Tetherin

    Plasmid

    #41072

    Depositor

    Paul Spearman

    Article

    Hammonds et al J Virol. 2012 Feb;86(4):2259-72. Epub 20

    Insert

    Tetherin (BST2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    137S, 144S

    Promoter

    CMV

    Available Since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only