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DS-NMcas

Plasmid

#48646

Purpose

Bacterial N. meningitidis Cas9 (NM) + tracrRNA expression, cloDF13/spectinomycin

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Inserts

Cas9
NM tracrRNA

Use

CRISPR

Promoter

NM tracrRNA promoter and proC

Available Since

Nov. 4, 2013

Availability

Academic Institutions and Nonprofits only

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pRK5-mWnt7b

Plasmid

#42283

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Wnt7b (Wnt7b Mouse)

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3-LAP2-YFP-NLS

Plasmid

#25847

Depositor

Alice Ting

Article

Uttamapinant et al Proc Natl Acad Sci U S A. 2010 Jun 15. 1

Insert

LAP2-YFP-NLS

Tags

His and NLS

Expression

Mammalian

Available Since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only

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AL13Rb

Plasmid

#161006

Purpose

PP7-tagged reporter without a promoter

Depositor

Krishna Niyogi

Article

Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

Type

Empty backbone

Tags

PP7 and H2B-mScarlet

Expression

Plant

Available Since

Dec. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pGL4_NFAT-RE-CMV-BC0547-luc2

Plasmid

#214665

Purpose

barcoded assay, Ca2+ sensor

Depositor

Michael Wehr

Article

Popovic et al STAR Protoc. 2024 Apr 16;5(2):102987. do

Insert

6x clustered NFAT element with barcode 0547

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pSIR-neo

Plasmid

#51128

Purpose

Self-inactivating retrovirus vector containing a neomycin-resistance gene

Depositor

Hodaka Fujii

Article

Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 19, 2014

Availability

Academic Institutions and Nonprofits only

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pSL4369 (pEffector_TnpB2,ωRNA)

Plasmid

#210871

Purpose

Expresses GstTnpB2 and native ωRNA from constitutive promoters

Depositor

Samuel H. Sternberg

Article

Meers et al Nature. 2023 Sep 27. doi: 10.1038/s41586

Insert

GstTnpB2

Expression

Bacterial

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pCRL1

Plasmid

#153997

Purpose

sgRNA in vitro transcription plasmid.

Depositor

Shengdar Tsai

Article

Lazzarotto et al Nat Protoc. 2018 Nov;13(11):2615-2642. d

Type

Empty backbone

Use

CRISPR; Sgrna under a t7 promoter for in vitro tr…

Promoter

T7

Available Since

June 29, 2020

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-Sapphire

Plasmid

#96950

Purpose

Retroviral plasmid containing Sapphire for labeling of cells

Depositor

Lee Grimes

Article

Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

Insert

Sapphire fluorescent protein

Expression

Mammalian

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pGL0_24 [pBBR1-UP ORI]

Plasmid

#198937

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pBBR1-UP ORI

Use

Synthetic Biology

Mutation

S100L (C299T)

Available Since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pmTurquoise-N1

Plasmid

#60559

Purpose

Expresses the cyan fluorescent protein variant mTurquoise

Depositor

Dorus Gadella

Article

Goedhart et al Nat Methods. 2010 Feb;7(2):137-9. doi: 1

Insert

mTurquoise

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pEPV-DsRed2-ER-5

Plasmid

#170571

Purpose

Expresses DsRed2 targeted to the ER lumen with an N-terminal EPV tripeptide after signal peptide cleavage.

Depositor

Ke Xu

Article

Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

Insert

EPV-DsRed2-ER-5

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

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AL12R

Plasmid

#161007

Purpose

PP7-tagged reporter without a promoter

Depositor

Krishna Niyogi

Article

Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

Type

Empty backbone

Tags

PP7 and H2B-mScarlet

Expression

Plant

Available Since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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mVenus-PA-MKI

Plasmid

#65457

Purpose

photoactivatable myosin light-chain kinase inihibition

Depositor

Klaus Hahn

Article

Yi et al ACS Synth Biol. 2014 Nov 21;3(11):788-95

Insert

MKI

Expression

Mammalian

Promoter

CMV

Available Since

July 16, 2015

Availability

Academic Institutions and Nonprofits only
pGL1_4

Plasmid

#199036

Purpose

Level 1 plasmid, barcoded ORI

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pIP501 ORI plus NGS barcode 4

Use

Synthetic Biology

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pET22-HT/spoR

Plasmid

#73631

Purpose

Produces Escherichia coli guanylate kinase (SpoR), N-terminal His6 tag

Depositor

T. J. Kappock, James Williamson

Article

Schultheisz et al ACS Chem Biol. 2008 Aug 15;3(8):499-511.

Insert

guanylate kinase

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pBEL2108

Plasmid

#201969

Purpose

ORBIT integrating plasmid for C-terminal tagging with eGFP containing a 3C protease cleavage site.

Depositor

Gira Bhabha, Damian Ekiert

Article

Chen et al Res Sq. 2023 Jan 10:rs.3.rs-2412186. doi

Insert

attB-3C-eGFP-4xGly-TEV-Flag-6xHis

Use

Orbit integrating plasmid

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pSL5200 (pDonor_ISGst3)

Plasmid

#210875

Purpose

Harbors a native ISGst3 transposon derived form Geobacillus stearothermophilus DSM 458

Depositor

Samuel H. Sternberg

Article

Meers et al Nature. 2023 Sep 27. doi: 10.1038/s41586

Insert

ISGst3

Expression

Bacterial

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pRK5-mWnt2b

Plasmid

#42275

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Wnt2b (Wnt2b Mouse)

Expression

Mammalian

Available Since

March 28, 2013

Availability

Academic Institutions and Nonprofits only
pE1B-C-F/Sox10-MCS5

Plasmid

#20244

Depositor

William Pavan

Article

Antonellis et al PLoS Genet. 2008 . 4(9):e1000174.

Insert

Sox10-MCS5

Expression

Mammalian

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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