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We narrowed to 8,913 results for: Ott;

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Showing: 6541 - 6560 of 8913 results

  1. pLEX307_CR-PDHA2S291A

    Plasmid
    #115203
    Purpose
    Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291A into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')
    Depositor
    Mohan Babu
    Article
    Moutaoufik et al iScience. 2019 Sep 4;19:1114-1132. doi:
    Insert
    CR (CRISPR/Cas9-resistant)-PDHA2S291A (PDHA2 Human)
    Use
    Lentiviral
    Mutation
    c.321C>T (silent when translated to protein, c…
    Promoter
    EF1alpha
    Available Since
    March 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    115203-plasmid-map-sequence-id-232429

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  12. pcDNA3.3_CoV2_D18

    Plasmid
    #170442
    Purpose
    Express SARS-CoV-2 WT spike protein with an 18aa deletion on the C-terminal tail. Used for SARS2 pseudotyped virus production.
    Depositor
    David Nemazee
    Article
    Rogers et al Science. 2020 Aug 21;369(6506):956-963.
    Insert
    SARS-CoV-2 spike D18 (S SARS-CoV-2, USA-WA1/2020 strain)
    Tags
    NA
    Expression
    Mammalian
    Mutation
    18aa deletion in c-terminal tail
    Promoter
    CMV
    Available Since
    July 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    170442-plasmid-map-sequence-id-337888

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  19. pCMV-T7-TadCBEd-SpCas9(LWKYQS)-P2A-EGFP (BKS1014)

    Plasmid
    #223127
    Purpose
    pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with LWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    human codon optimized TadA-CDd-SpCas9(LWKYQS)-2xUGI-P2A-EGFP
    Use
    CRISPR
    Tags
    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd
    Expression
    Mammalian
    Mutation
    TadA-CDd mutations; nSpCas9(D10A/LWKYQS)=D1135L, …
    Promoter
    CMV and T7
    Available Since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    223127-plasmid-map-sequence-id-447695

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  20. pTBL1269 pLenti-CHYRON17-pEF1a(short)-Luc2-P2A-tdTomato-T2A-TdT

    Plasmid
    #163643
    Purpose
    Makes a lentivirus that expresses hgRNA with 17nt spacer and Luc2, tdTomato, and human TdT.
    Depositor
    Chang Liu
    Article
    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d
    Inserts
    pU6-CHYRON17 hgRNA
    Luc2-P2A-tdTomato-T2A-human TdT (DNTT Human)
    Use
    Lentiviral
    Tags
    Luc2-P2A-tdTomato-T2A
    Mutation
    The SpCas9 sgRNA constant region is mutated to ma…
    Promoter
    EFS and pU6
    Available Since
    Feb. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    163643-plasmid-map-sequence-id-325021

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Showing: 6541 - 6560 of 8913 results