We narrowed to 8,324 results for: gnal

1. pCK014

   Plasmid

   #105346

   Purpose

   Bacterial translocation fo proteins into plant cells

   Depositor

   Johannes Stuttmann

   Article

   Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

   Insert

   secretion signal, ccdB cassette
   

   Use

   Bacterial secretion

   Tags

   Expression

   Mutation

   BsaI restriction sites removed

   Promoter

   Available Since

   May 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDH_SBP-EGFP-Ecadherin

   Plasmid

   #65290

   Purpose

   Ecadherin reporter only (no hook) (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Ecadherin
   

   Use

   Lentiviral

   Tags

   EGFP and IL-2 Signal Sequence and Streptavidin Bi…

   Expression

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-Syn-VARNAM

   Plasmid

   #115554

   Purpose

   Red fluorescent voltage indicator for neuronal expression

   Depositor

   Vincent Pieribone

   Article

   Kannan et al Nat Methods. 2018 Dec;15(12):1108-1116.

   Insert

   VARNAM
   

   Use

   AAV

   Tags

   Golgi and ER export signal

   Expression

   Mutation

   Promoter

   human Synapsin

   Available Since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CMV-dSa-VPR mini.-2X snRP1

   Plasmid

   #99688

   Purpose

   Expresses dSa Cas9 fused to optimized combination of truncated activation domains with 34 nt dual snRP1 poly adenylation signal

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9
   

   Use

   AAV

   Tags

   VPR mini

   Expression

   Mutation

   dead Cas9

   Promoter

   Available Since

   Oct. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTEV5_ RpMatB_1

   Plasmid

   #78205

   Purpose

   Expression of double-cysteine variant of Malonyl-coenzyme A synthetase, RpMatB, as scaffold for ATP biosensor

   Depositor

   Martin Webb

   Article

   Vancraenenbroeck et al ACS Chem Biol. 2015 Nov 20;10(11):2650-7

   Insert

   matB (His6 C106A R286C Q457C K488A)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   C106A R286C Q457C K488A

   Promoter

   T7

   Available Since

   July 5, 2016

   Availability

   Academic Institutions and Nonprofits only

6. FKBP_LD 0_TEV

   Plasmid

   #58877

   Purpose

   MESA protease chain with Rapamycin FKBP ectodomain and no linker domain

   Depositor

   Joshua Leonard

   Article

   Daringer et al ACS Synth Biol. 2014 Mar 11.

   Insert

   MESA protease chain with FKBP rapamycin-binding ectodomain, TEV protease and mCherry fusion
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJEP205-AAV-TRE3G-GFP-pA

   Plasmid

   #164789

   Purpose

   AAV vector backbone designed to express GFP from a TRE3G promoter. It also contains a poly-Adenylation Signal (pA)

   Depositor

   Jonathan Ploski

   Article

   Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

   Insert

   Green Fluorescent protein (GFP)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-ICUE3-NLS

   Plasmid

   #138218

   Purpose

   Nuclear-targeted FRET-based cAMP indicator.

   Depositor

   Jin Zhang

   Article

   Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

   Insert

   ICUE3-NLS
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Feb. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPW3756 : CMVd3-ERmembrane-HaloTag-KKMP

   Plasmid

   #185678

   Purpose

   Low-level transient expression of a single HaloTag protein with a C-terminal KKMP ER retention signal, targeted to the ER membrane.

   Depositor

   Peter Walter

   Article

   Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

   Insert

   ERmembrane-HaloTag-KKMP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDH_SBP-mCherry-GPI

    Plasmid

    #65299

    Purpose

    synchronize trafficking of mCherry-GPI from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    GPI anchor
    

    Use

    Lentiviral

    Tags

    IL-2 Signal Sequence and Streptavidin Binding Pro…

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEF-pIgH-ESCargo(FTV,Crimson)

    Plasmid

    #140162

    Purpose

    Expresses a regulatable secretory cargo for mammalian cells

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    piGH-FTVNTT-E2-Crimson-FKBP(LV)(C22V)
    

    Use

    Tags

    ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available Since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. RNF168-Halo HRD

    Plasmid

    #207080

    Purpose

    Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RNF168 locus.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    HaloTag followed by a PolyA signal and PuroR cassette flanked by human RNF168 locus sequences
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    None

    Available Since

    May 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-nDsRedFL-intron

    Plasmid

    #177804

    Purpose

    An intron is inserted into the DsRed gene with a nuclear transfer signal and a 3xFlag tag. The intron can be digested with EcoRV and any pre-miRNA can be inserted.

    Depositor

    Rikako Sanuki

    Article

    Suzuki et al Genes Cells. 2020 Nov;25(11):741-752. do

    Insert

    intron
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. FLAG-hLRP6-opt(20-1613)-9xHis_MESD_pFastBacDual

    Plasmid

    #216377

    Purpose

    Baculovirus transfer vector to co-express FLAG-LRP6-His (human sequence, codon-optimized for insect cell expression) and MESD chaperone (human sequence)

    Depositor

    William Weis

    Insert

    LDL receptor related protein 6 (LRP6 Human)
    

    Use

    Baculovirus

    Tags

    9xHis and HA signal sequence-FLAG-3C cleavage sit…

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin (LRP6)/p10 (MESD)

    Available Since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDS257

    Plasmid

    #34971

    Purpose

    Expresses Mid2(SS/TM), containing only the signal sequence and transmembrane helix, fused to GFP and LOVpep.

    Depositor

    Michael Glotzer

    Article

    Strickland et al Nat Methods. 2012 Mar 4. doi: 10.1038/nm

    Insert

    Mid2(SS/TM)-GFP-LOVpep
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDGB3_alpha1-pAtUBQ10:3xNLS-HA-LRR-NEL-mcherry-Tnos

    Plasmid

    #211841

    Purpose

    Plant transient expression cassette for the E3-DART ligase containing a nuclear localization signal.

    Depositor

    Marcela Rojas-Pierce

    Article

    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

    Insert

    LRR-NEL
    

    Use

    Synthetic Biology

    Tags

    3xNLS-HA and mCherry

    Expression

    Plant

    Mutation

    Promoter

    UBIQUITIN10

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX330-NEAT1m_v1

    Plasmid

    #97083

    Purpose

    Encodes an sgRNA that creates a DSB immediately downstream the poly(A) signal of human NEAT1_1 isoform

    Depositor

    Archa Fox

    Article

    Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

    Insert

    sgRNA NEAT1m_v1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available Since

    July 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-CaMKIIa-C1C2-TS-EYFP

    Plasmid

    #35520

    Depositor

    Karl Deisseroth

    Article

    Kato et al Nature. 2012 Jan 22. doi: 10.1038/nature

    Insert

    ChR1-ChR2 Chimera
    

    Use

    Lentiviral

    Tags

    Trafficking signal and EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKIIa

    Available Since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pME-nlsGFP-P2A

    Plasmid

    #80808

    Purpose

    nuclear localization signal fused to N-terminus of GFP (nlsGFP) without stop codon, followed by P2A

    Depositor

    Philip Washbourne

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    minimal cytomegalovirus immediate early enhancer/promoter
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDH_SBP-mCherry-Ecadherin

    Plasmid

    #65291

    Purpose

    Ecadherin reporter only (no hook) (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Ecadherin
    

    Use

    Lentiviral

    Tags

    IL-2 Signal Sequence and Streptavidin Binding Pro…

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge