We narrowed to 59,355 results for: cloning

1. P2A-mCherry-EhT backbone

   Plasmid

   #215549

   Purpose

   An empty backbone for cloning of homology arms; ITR-flanked, EF1alpha-driven expression of hygromycin-NLS-tdTomato

   Depositor

   Kai Wang

   Article

   Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. DL016:CMV::eGFP-iPAK4

   Plasmid

   #177881

   Purpose

   eGFP-IPAK4 fusion with CMV promoter, cloned into a lentivirus backbone

   Depositor

   Adam Cohen

   Article

   Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   iPAK4
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. CMV-HA-hTAF4

   Plasmid

   #17983

   Depositor

   Naoko Tanese

   Article

   Tanese et al Proc Natl Acad Sci U S A. 1996 Nov 26. 9

   Insert

   TAF4 (TAF4 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   missing 5' end

   Available Since

   Aug. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

4. puc19-pCas13a

   Plasmid

   #118963

   Purpose

   Intermediate/cloning vector that can be used for sub-cloning into final destination vectors based on one's requirements.

   Depositor

   Magdy Mahfouz

   Article

   Aman et al Genome Biol. 2018 Jan 4;19(1):1. doi: 10

   Insert

   Cas13a
   

   Expression

   Plant

   Available Since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCPP5052

   Plasmid

   #128729

   Purpose

   Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

   Depositor

   Alan Collmer

   Article

   Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

   Insert

   hopAM1
   

   Expression

   Bacterial

   Available Since

   Aug. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-15b PAI-RBP

   Plasmid

   #45390

   Depositor

   Thomas Gelehrter

   Article

   Heaton et al J Biol Chem. 2001 Feb 2;276(5):3341-7. E

   Insert

   PAI-RBP (SERBP1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   June 20, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pGuide

   Plasmid

   #64711

   Purpose

   Cloning and expression of Streptococcus pyogenes guide RNA

   Depositor

   Kiran Musunuru

   Article

   Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.

   Insert

   Streptococcus pyogenes guide RNA
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   May 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR221-kcnk18

   Plasmid

   #209000

   Purpose

   Riboprobe generation

   Depositor

   GuangJun Zhang

   Article

   Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

   Insert

   kcnk18 (kcnk18 Zebrafish)
   

   Use

   Cloning

   Promoter

   T7

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBT13-splitD

   Plasmid

   #122597

   Purpose

   clone M13 phage by SapI Golden Gate

   Depositor

   David Liu

   Article

   Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

   Insert

   M13 bacteriophage genes
   

   Expression

   Bacterial

   Available Since

   July 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEN_TTmcs

    Plasmid

    #25755

    Purpose

    Entry vector with TRE-tight promoter followed by multiple cloning site.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Type

    Empty backbone

    Use

    Entry vector

    Available Since

    July 14, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pQTEV-TAGLN2

    Plasmid

    #31569

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    transgelin 2 (TAGLN2 Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBT137-splitC

    Plasmid

    #122605

    Purpose

    clone M13 phage by SapI Golden Gate

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    M13 bacteriophage genes
    

    Expression

    Bacterial

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR221_SLC22A2

    Plasmid

    #131964

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Mocking et al Int J Mol Sci. 2022 Jan 21;23(3):1203. d

    Insert

    SLC22A2 (SLC22A2 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 21, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pMG343

    Plasmid

    #176183

    Purpose

    preassembled yeast MoClo level-1 backbone with toxic gene dropout cassette (ccdB and TPK2) and URA3 marker

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Insert

    TPK2+ccdB dropout cassette
    

    Expression

    Yeast

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. CROPseq-Puro-24xMS2

    Plasmid

    #212629

    Purpose

    MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles

    Depositor

    Paul Blainey

    Article

    Najia et al bioRxiv 2024.10.01.616098

    Insert

    EF1a-PuroR-24xMS2-WPRE-hU6-sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCA_e-1

    Plasmid

    #202096

    Purpose

    Receiver cloning vector for even-1 level constructs. Contains lacZ for blue/white screening.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Mutation

    None

    Available Since

    Aug. 23, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pCA_o-1

    Plasmid

    #202091

    Purpose

    Receiver cloning vector for odd-1 level constructs. Contains lacZ for blue/white screening.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Mutation

    None

    Available Since

    Aug. 23, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pBT137-splitD

    Plasmid

    #122606

    Purpose

    clone M13 phage by SapI Golden Gate

    Depositor

    David Liu

    Article

    Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

    Insert

    M13 bacteriophage genes
    

    Expression

    Bacterial

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. PAGFP_NS2B3

    Plasmid

    #195755

    Purpose

    Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.

    Depositor

    Partha Mondal

    Article

    Varghese et al bioRxiv 2022.12.18.520514

    Insert

    Dengue (Type-2) NS2B3
    

    Tags

    PA-GFP

    Expression

    Mammalian

    Mutation

    No mutations

    Promoter

    CMV

    Available Since

    Feb. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJEC819

    Plasmid

    #190815

    Purpose

    CRISPRa backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.

    Depositor

    James Chappell

    Article

    Ameruoso et al Nucleic Acids Res. 2022 Jul 22;50(13):77

    Insert

    dCas9-XTEN-rpoA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    XTEN (linker), followed by the N-terminal domain …

    Promoter

    SP30

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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