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pAV-U6+27-Linear-3_PolyU(1)_Tornado-Corn

Plasmid

#159503

Purpose

Tests for the impact of 1 uracil in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-3_PolyU(1)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6-27

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Linear-2_PolyU(7)_Tornado-Corn

Plasmid

#159493

Purpose

Tests for the impact of 7 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-2_PolyU(7)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6-27

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Linear-2_PolyU(4)_Tornado-Corn

Plasmid

#159490

Purpose

Tests for the impact of 4 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-2_PolyU(4)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6-27

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Linear-2_PolyU(2)_Tornado-Corn

Plasmid

#159488

Purpose

Tests for the impact of 2 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-2_PolyU(2)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6-27

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Linear-1_PolyU(4)_Tornado-Corn

Plasmid

#159482

Purpose

Tests for the impact of 4 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-1_PolyU(4)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6-27

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Linear-1_PolyU(5)_Tornado-Corn

Plasmid

#159483

Purpose

Tests for the impact of 5 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-1_PolyU(5)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6-27

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Linear-1_PolyU(6)_Tornado-Corn

Plasmid

#159484

Purpose

Tests for the impact of 6 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-1_PolyU(6)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6-27

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Linear-1_PolyU(7)_Tornado-Corn

Plasmid

#159485

Purpose

Tests for the impact of 7 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-1_PolyU(7)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6-27

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Linear-1_PolyU(8)_Tornado-Corn

Plasmid

#159486

Purpose

Tests for the impact of 8 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-1_PolyU(8)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6-27

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pRS314-U6-A34G/A35G/G96C/A97C/U100C/U101C

Plasmid

#81239

Purpose

U6 telestem mutant (A34G/A35G/G96C/A97C/U100C/U101C) for in vivo studies

Depositor

Aaron Hoskins

Article

Rodgers et al Nucleic Acids Res. 2016 Dec 15;44(22):10

Insert

U6 RNA with A34G/A35G/G96C/A97C/U100C/U101C mutation (snR6 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

U6 telestem mutant (A34G/A35G/G96C/A97C/U100C/U10…

Promoter

Available since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-U6-NmeCas9-TLR-MCV1-sgRNA

Plasmid

#117407

Purpose

NmeCas9 sgRNA targeting TLR2.0

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

NmeCas9 sgRNA targeting TLR 2.0

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-U6-CjeCas9-TLR-MCV1-sgRNA

Plasmid

#117408

Purpose

CjeCas9 sgRNA targeting TLR2.0

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

CjeCas9 sgRNA targeting TLR 2.0

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-U6-SauCas9-TLR-MCV1-sgRNA

Plasmid

#117409

Purpose

SauCas9 sgRNA targeting TLR2.0

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

SauCas9 sgRNA targeting TLR 2.0

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-U6-LbCas12a-TLR-MCV1-gRNA

Plasmid

#117410

Purpose

LbCas12a-gRNA targeting TLR2.0

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

LbCas12a gRNA targeting TLR 2.0

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Dec. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pFB-U6-control-gRNA-hSyn-mCherry-WPRE-SV40pA

Plasmid

#128347

Purpose

pAAV encoding control gRNA for CRISPR gRNAs listed above

Depositor

Ryan Drenan

Article

Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

Insert

control (negative)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pFB-U6-Chrnb4-gRNA-hSyn-mCherry-WPRE-SV40pA

Plasmid

#128345

Purpose

pAAV encoding gRNA sequence for loss-of-function indels

Depositor

Ryan Drenan

Article

Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

Insert

Chrnb4 (Chrnb4 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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pFB-U6-Chrna6-gRNA-hSyn-mCherry-WPRE-SV40pA

Plasmid

#128341

Purpose

pAAV encoding gRNA sequence for loss-of-function indels

Depositor

Ryan Drenan

Article

Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

Insert

Chrna6 (Chrna6 Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6(BsmBI)-EF1a-BFP-BSD-Nkx2-5

Plasmid

#117137

Purpose

Expression vector encoding BFP-BSD-Nkx2-5

Depositor

Kosuke Yusa

Article

Yu et al Stem Cells. 2019 Apr 1. doi: 10.1002/ste

Insert

Nkx2-5 CDS (Nkx2-5 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

modified BFP

Promoter

human U6 promoter, human EF1a promoter

Available since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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pSLQ1852-5 pHR: U6-SpsgCD95-3 CMV-EGFP

Plasmid

#84253

Purpose

Expresses Sp sgCD95-3 gRNA with an EGFP fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sp sgCD95-3

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pSLQ1852-3 pHR: U6-SpsgCD95-2 CMV-EGFP

Plasmid

#84252

Purpose

Expresses Sp sgCD95-2 gRNA with an EGFP fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sp sgCD95-2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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Showing: 641 - 660 of 4687 results

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