Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 13,031 results for: Hal

Clear keywords
Showing: 1281 - 1320 of 13031 results

  1. JL054: pPB_NFκB::GFP-GEMINI(A)-P1N4_TRE::HaloTag-GEMINI(A)_UbC::rtTA3-IRES-PuroR

    Plasmid
    #228884
    Purpose
    PiggyBac plasmid expressing the destabilized GFP-GEMINI(A) under the control of a synthetic NFκB-responsive promotor, and rtTA3 and PuroR under the control of a UbC promoter.
    Depositor
    Dingchang Lin
    Article
    Yan et al Nature. 2026 Mar 3. doi: 10.1038/s41586-
    Insert
    GFP-fused GEMINI(A), HaloTag-fuse GEMINI(A)
    Expression
    Mammalian
    Promoter
    pNFκB; TRE
    Available Since
    Feb. 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    228884-plasmid-map-sequence-id-454316

    Enlarge

  2. YY171: pPB_NFκB::GFP-GEMINI(A)_TRE::HaloTag-GEMINI(A)_UbC::rtTA3-IRES-PuroR

    Plasmid
    #228883
    Purpose
    PiggyBac plasmid expressing the GFP-GEMINI(A) under the control of a synthetic NFκB-responsive promotor, and rtTA3 and PuroR under the control of a UbC promoter.
    Depositor
    Dingchang Lin
    Article
    Yan et al Nature. 2026 Mar 3. doi: 10.1038/s41586-
    Insert
    GFP-fused GEMINI(A), HaloTag-fuse GEMINI(A)
    Expression
    Mammalian
    Promoter
    pNFκB; TRE
    Available Since
    Feb. 3, 2026
    Availability
    Academic Institutions and Nonprofits only
    228883-plasmid-map-sequence-id-454808

    Enlarge

  4. pCMV-scFv-GCN4-sfGFP-tdP2A-Frankenbody-FLAG-mScarlet3-IRESv4-HaloTag-tdMCP

    Plasmid
    #241141
    Purpose
    Expresses Anti-GCN4 scFv-sfGFP, anti-FLAG frankenbody-mEGFP, and HaloTag-tdMCP to track mature and nascent SunTag and FLAG-tagged proteins and MS2-tagged mRNA
    Depositor
    Tim Stasevich
    Article
    Galindo et al Mol Biol Cell. 2025 Jun 1;36(6):mr6. doi
    Inserts
    Anti-GCN4 scFv
    anti-FLAG frankenbody
    tdMCP
    Tags
    HaloTag, mEGFP, and sfGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 3, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    241141-plasmid-map-sequence-id-485239

    Enlarge

  5. pCDNA5/FRT-Lyn11-mEGFP-cpHalo∆-(GGS)9-FKBP-P2A-Hpep3-(GGS)3-FRB-mScarlett

    Plasmid
    #205703
    Purpose
    CMV driven co-expression of split HaloTag FKBP- and FRB-fusions (Lyn11-EGFP-FKBP-cpHalo∆ and Hpep1-FRB-mScarlett) in mammalian cell lines
    Depositor
    Kai Johnsson
    Article
    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.
    Insert
    Lyn11-EGFP-FKBP-cpHalo∆-P2A-Hpep1-FRB-mScarlett
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    205703-plasmid-map-sequence-id-423137

    Enlarge

  6. pAAVS1_TREtight-GEM2-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

    Plasmid
    #197061
    Purpose
    Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM2-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)
    Depositor
    Julia Mahamid
    Article
    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.
    Inserts
    Encapsulin
    Adaptor Protein
    Use
    CRISPR
    Tags
    FRB and HaloTag
    Expression
    Mammalian
    Mutation
    Codon-optimised for human cell expression
    Available Since
    Sept. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    197061-plasmid-map-sequence-id-402602

    Enlarge

  7. pAAVS1_TREtight-FRB-GEM23-Halo-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

    Plasmid
    #197065
    Purpose
    Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of FRB-GEM23-Halo and Adaptor Protein (FKBP-SNAP-vhhGFP4)
    Depositor
    Julia Mahamid
    Article
    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.
    Inserts
    I3-01
    Adaptor Protein
    Use
    CRISPR
    Tags
    FRB and HaloTag
    Expression
    Mammalian
    Mutation
    Codon-optimised for human cell expression
    Available Since
    Sept. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    197065-plasmid-map-sequence-id-402601

    Enlarge

  8. pAAVS1_TREtight-GEM22-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

    Plasmid
    #197064
    Purpose
    Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM22-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)
    Depositor
    Julia Mahamid
    Article
    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.
    Inserts
    Encapsulin
    Adaptor Protein
    Use
    CRISPR
    Tags
    FRB and HaloTag
    Expression
    Mammalian
    Mutation
    Codon-optimised for human cell expression
    Available Since
    Sept. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    197064-plasmid-map-sequence-id-402605

    Enlarge

  9. pAAVS1_TREtight-GEM4-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

    Plasmid
    #197062
    Purpose
    Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM4-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)
    Depositor
    Julia Mahamid
    Article
    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.
    Inserts
    Encapsulin
    Adaptor Protein
    Use
    CRISPR
    Tags
    FRB and HaloTag
    Expression
    Mammalian
    Mutation
    Codon-optimised for human cell expression
    Available Since
    Sept. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    197062-plasmid-map-sequence-id-402606

    Enlarge

  10. pAAVS1_TREtight-GEM7-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

    Plasmid
    #197063
    Purpose
    Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM7-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)
    Depositor
    Julia Mahamid
    Article
    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.
    Inserts
    Encapsulin
    Adaptor Protein
    Use
    CRISPR
    Tags
    FRB and HaloTag
    Expression
    Mammalian
    Mutation
    Codon-optimised for human cell expression
    Available Since
    Sept. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    197063-plasmid-map-sequence-id-408409

    Enlarge

  11. KL094 - pTRE3G-Flag-Halo-CTCF_WT-BGHpA-CAGGS-rtta3G-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt TIGRE donor

    Plasmid
    #232930
    Purpose
    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible Flag-Halo-mCTCF WT, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycin selection.
    Depositor
    Danny Reinberg
    Article
    Lucero et al bioRxiv, March 19, 2025
    Insert
    Flag-Halo-CTCF WT
    Use
    Mouse Targeting
    Expression
    Mammalian
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    232930-plasmid-map-sequence-id-466502

    Enlarge

  12. KL095 - pTRE3G-Flag-Halo-CTCF_ZF1_mutant-BGHpA-CAGGS-rtta3G-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt TIGRE donor

    Plasmid
    #232931
    Purpose
    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible Flag-Halo-mCTCF ZF1 mutant, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycin selection.
    Depositor
    Danny Reinberg
    Article
    Lucero et al bioRxiv, March 19, 2025
    Insert
    Flag-Halo-CTCF ZF1 deletion mutant
    Use
    Mouse Targeting
    Expression
    Mammalian
    Mutation
    Deleted amino acids 264-277
    Available Since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    232931-plasmid-map-sequence-id-466490

    Enlarge

  13. BH1352

    Plasmid
    #255502
    Purpose
    DERA enzyme production under control of T7 promoter and inducible lac operator
    Depositor
    Radhakrishnan Mahadevan
    Article
    Kim et al J Biol Chem. 2020 Jan 10;295(2):597-609.
    Insert
    2-deoxyribose-5-phosphate aldolase (DERA) from Halalkalibacterium halodurans (formerly Bacillus halodurans)
    Tags
    6xHis tag-TEV protease recognition site (ENLYFQ/G…
    Expression
    Bacterial
    Mutation
    WT
    Promoter
    T7
    Available Since
    May 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    255502-plasmid-map-sequence-id-509490

    Enlarge

  14. GS2.1/EC

    Plasmid
    #132568
    Purpose
    sgRNA targeting A. thaliana pds3, regulated by A. thaliana U6 polIII promoter. Cas9 with a C-terminal mApple fusion, egg cell-specific expression. Hygromycin selectable marker (hpt).
    Depositor
    Mathias Pribil
    Article
    Behrendorff et al Physiol Plant. 2020 Feb 25. doi: 10.1111
    Insert
    egg cell promoter, Cas9-mApple, pds3 sgRNA
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Promoter
    A. thaliana egg cell promoter
    Available Since
    Dec. 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    132568-plasmid-map-sequence-id-262634

    Enlarge

  15. pMC_HT7_BSDminus_F0

    Plasmid
    #225627
    Purpose
    Donor cassette plasmid for high-throughput tagging, encoding an HaloTag7 synthetic exon in frame 0, as well as a blasticidin resistance gene for selection of genomic integrants.
    Depositor
    Ophir Shalem
    Insert
    HT7 and BSD
    Use
    CRISPR
    Available Since
    April 14, 2026
    Availability
    Academic Institutions and Nonprofits only
    225627-plasmid-map-sequence-id-485001

    Enlarge

  16. pMC_HT7_BSDminus_F1

    Plasmid
    #225628
    Purpose
    Donor cassette plasmid for high-throughput tagging, encoding an HaloTag7 synthetic exon in frame 1, as well as a blasticidin resistance gene for selection of genomic integrants.
    Depositor
    Ophir Shalem
    Insert
    HT7 and BSD
    Use
    CRISPR
    Available Since
    April 14, 2026
    Availability
    Academic Institutions and Nonprofits only
    225628-plasmid-map-sequence-id-485504

    Enlarge

  17. pMC_HT7_BSDminus_F2

    Plasmid
    #225629
    Purpose
    Donor cassette plasmid for high-throughput tagging, encoding an HaloTag7 synthetic exon in frame 2, as well as a blasticidin resistance gene for selection of genomic integrants.
    Depositor
    Ophir Shalem
    Insert
    HT7 and BSD
    Use
    CRISPR
    Available Since
    April 14, 2026
    Availability
    Academic Institutions and Nonprofits only
    225629-plasmid-map-sequence-id-485002

    Enlarge

  18. pVZ_PcoaT_SQE1_THAS1

    Plasmid
    #103859
    Purpose
    Heterologous, cobalt-inducible expression of SQE1 and THAS1 from A. thaliana in Synechocystis sp. PCC6803
    Depositor
    Ilka Axmann
    Article
    Loeschcke et al PLoS One. 2017 Dec 27;12(12):e0189816. d
    Insert
    thalianol synthase 1 (THAS1 Mustard Weed)
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    inserted genes are codon optimized. 2nd amino aci…
    Promoter
    PcoaT (from Synechocystis sp. PCC 6803)
    Available Since
    July 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    103859-plasmid-map-sequence-id-217087

    Enlarge

  20. pEG302_22aa_SunTag_MQ1(Q147L)_g4+g10+g18

    Plasmid
    #172318
    Purpose
    CRISPR dCas9 SunTag system to target a variant of bacterial DNA methyltransferase MQ1 to install CG specific methylation to the FWA locus with three guide RNAs
    Depositor
    Steven Jacobsen
    Article
    Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118
    Insert
    g18_U6_g10_U6_g4_U6_NOS_MQ1(Q147L)_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available Since
    Sept. 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    172318-plasmid-map-sequence-id-343646

    Enlarge

  23. pUE001-Cas1:Dendra2Hfx

    Plasmid
    #234670
    Purpose
    encodes Cas1:Dendra2Hfx fusion in Haloferax volcanii under the tryptophan inducible p.tna promoter, trpA, ampR, shuttle vector for E.coli
    Depositor
    Ulrike Endesfelder
    Article
    Schrage et al bioRxiv 2025.02.24.639823
    Insert
    Cas1
    Use
    Archeal expression in haloferax volcanii
    Tags
    Dendra2Hfx
    Promoter
    p.tna
    Available Since
    March 11, 2026
    Availability
    Academic Institutions and Nonprofits only
    234670-plasmid-map-sequence-id-468194

    Enlarge

  24. pUE001-FtsZ1:Dendra2Hfx

    Plasmid
    #234671
    Purpose
    encodes FtsZ1:Dendra2Hfx fusion in Haloferax volcanii under the tryptophan inducible p.tna promoter, trpA, ampR, shuttle vector for E.coli
    Depositor
    Ulrike Endesfelder
    Article
    Schrage et al bioRxiv 2025.02.24.639823
    Insert
    FtsZ1
    Use
    Archeal expression in haloferax volcanii
    Tags
    Dendra2Hfx
    Promoter
    p.tna
    Available Since
    March 11, 2026
    Availability
    Academic Institutions and Nonprofits only
    234671-plasmid-map-sequence-id-468195

    Enlarge

Showing: 1281 - 1320 of 13031 results