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Showing: 1281 - 1320 of 1645 results

  1. pHL718

    Plasmid
    #62826
    Purpose
    Lim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + PLtetO-1::ipex::mCherry::T1 terminator + ColE1
    Depositor
    Han Lim
    Article
    Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv
    Inserts
    gfp
    mCherry
    Use
    Synthetic Biology
    Tags
    ipex leader and ompC leader
    Expression
    Bacterial
    Mutation
    Promoter
    pLlacO-1 and pLtetO-1
    Available since
    Aug. 14, 2015
    Availability
    Academic Institutions and Nonprofits only

  2. JAB3479

    Plasmid
    #190417
    Purpose
    Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_6XAO-2XPO::TetR-NLS-19St_proT::GFPint-ADHt)
    Depositor
    Jennifer Brophy, Jose Dinneny
    Article
    Brophy et al Science. 2022 Aug 12;377(6607):747-751.
    Insert
    proUBQ10::PIP2A-mCherry-NOSt_6XAO-2XPO::TetR-NLS-19St_proT::GFPint-ADHt
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    May 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    190417-plasmid-map-sequence-id-393617

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  3. JAB3278

    Plasmid
    #190402
    Purpose
    Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-ERF2(AD)-19St_1XAmtRO(M2)::GFPint-NLS-ADHt)
    Depositor
    Jennifer Brophy, Jose Dinneny
    Article
    Brophy et al Science. 2022 Aug 12;377(6607):747-751.
    Insert
    proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-ERF2(AD)-19St_1XAmtRO(M2)::GFPint-NLS-ADHt
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    May 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    190402-plasmid-map-sequence-id-393458

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  4. JAB3616

    Plasmid
    #190426
    Purpose
    Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_6XAmtRO-2XPhlFO::TetR-NLS-19St_proT::GFPint-ADHt)
    Depositor
    Jennifer Brophy, Jose Dinneny
    Article
    Brophy et al Science. 2022 Aug 12;377(6607):747-751.
    Insert
    proUBQ10::PIP2A-mCherry-NOSt_6XAmtRO-2XPhlFO::TetR-NLS-19St_proT::GFPint-ADHt
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    190426-plasmid-map-sequence-id-391175

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  5. JAB2601

    Plasmid
    #190388
    Purpose
    Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_3XAmtRO::GFPint-NLS-ADHt)
    Depositor
    Jennifer Brophy, Jose Dinneny
    Article
    Brophy et al Science. 2022 Aug 12;377(6607):747-751.
    Insert
    proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_3XAmtRO::GFPint-NLS-ADHt
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    190388-plasmid-map-sequence-id-391162

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  6. JAB2600

    Plasmid
    #190387
    Purpose
    Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_2XAmtRO::GFPint-NLS-ADHt)
    Depositor
    Jennifer Brophy, Jose Dinneny
    Article
    Brophy et al Science. 2022 Aug 12;377(6607):747-751.
    Insert
    proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_2XAmtRO::GFPint-NLS-ADHt
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    190387-plasmid-map-sequence-id-391161

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  7. JAB2599

    Plasmid
    #190386
    Purpose
    Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_1XAmtRO::GFPint-NLS-ADHt)
    Depositor
    Jennifer Brophy, Jose Dinneny
    Article
    Brophy et al Science. 2022 Aug 12;377(6607):747-751.
    Insert
    proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_1XAmtRO::GFPint-NLS-ADHt
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    April 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    190386-plasmid-map-sequence-id-389752

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  8. pHL391

    Plasmid
    #62821
    Purpose
    Lim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + PLtetO-1::RBS (st7) hfq::mCherry::T1 terminator + ColE1
    Depositor
    Han Lim
    Article
    Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv
    Inserts
    gfp
    mCherry
    Use
    Synthetic Biology
    Tags
    hfp leader, ompC leader, and st7 RBS
    Expression
    Bacterial
    Mutation
    Promoter
    pLlacO-1 and pLtetO-1
    Available since
    Aug. 14, 2015
    Availability
    Academic Institutions and Nonprofits only

  9. pHL518

    Plasmid
    #62823
    Purpose
    Lim Lab plasmid: AmpR + PLlacO-1::micC::mCherry::T1 terminator + PLtetO-1m9::OmpC::gfp::T1T2 terminator + ColE1
    Depositor
    Han Lim
    Article
    Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv
    Inserts
    mCherry
    gfp
    Use
    Synthetic Biology
    Tags
    m9::ompC leader and micC leader
    Expression
    Bacterial
    Mutation
    Promoter
    pLlacO-1 and pLtetO-1
    Available since
    Aug. 14, 2015
    Availability
    Academic Institutions and Nonprofits only

  10. pLVX-TIGHT-CYCLINF-Tet-OFF

    Plasmid
    #32977
    Depositor
    Michele Pagano
    Insert
    CYCLIN F (CCNF Human)
    Use
    Lentiviral
    Tags
    Flag and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    P-TIGHT
    Available since
    Feb. 13, 2012
    Availability
    Academic Institutions and Nonprofits only

  13. pKG402

    Plasmid
    #69760
    Purpose
    Retroviral expression of CENP-T aa 256-561
    Depositor
    Iain Cheeseman
    Insert
    CENP-T aa 256-561 (CENPT Human)
    Use
    Retroviral
    Tags
    mCherry-TEV-S
    Expression
    Mammalian
    Mutation
    e.coli optimized sequence
    Promoter
    Available since
    Nov. 6, 2015
    Availability
    Academic Institutions and Nonprofits only

  14. pKG210

    Plasmid
    #69761
    Purpose
    Retroviral expression of CENP-T aa 1-375
    Depositor
    Iain Cheeseman
    Insert
    CENP-T aa 1-375 (CENPT Human)
    Use
    Retroviral
    Tags
    LacI-NLS and mCherry
    Expression
    Mammalian
    Mutation
    e.coli optimized sequence
    Promoter
    Available since
    Nov. 6, 2015
    Availability
    Academic Institutions and Nonprofits only

  15. pCVL TLR 3.1 (Sce target) Ef1a iRFP Puro

    Plasmid
    #50417
    Purpose
    Fluorescent reporter for DNA cleavage/repair in mammalian cells expressing iRFP with selectable puromycin marker.
    Depositor
    Andrew Scharenberg
    Inserts
    eGFP with I-Sce I TS
    +3 mCherry
    Use
    Lentiviral
    Tags
    T2A dislinker and iRFP
    Expression
    Mammalian
    Mutation
    embedded I-Sce I TS from 163-185
    Promoter
    SFFV
    Available since
    Jan. 14, 2014
    Availability
    Academic Institutions and Nonprofits only
    50417-plasmid-map-sequence-id-75349

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  16. pKG212

    Plasmid
    #69758
    Purpose
    Retroviral expression of CENP-T aa 1-242
    Depositor
    Iain Cheeseman
    Insert
    CENP-T aa 1-242 (CENPT Human)
    Use
    Retroviral
    Tags
    LacI-NLS and mCherry
    Expression
    Mammalian
    Mutation
    e.coli optimized sequence
    Promoter
    Available since
    Nov. 18, 2015
    Availability
    Academic Institutions and Nonprofits only

  18. pET24a-LaM4-3xTS

    Plasmid
    #162777
    Purpose
    Bacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to three tyrosine sulfation (TS) motifs. LaM4-3xTS also contains a T7, HA, BAP and His6 epitope
    Depositor
    Martin Spiess
    Article
    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii
    Insert
    anti-mCherry nanobody fused to three TS sites, T7, HA, BAP and His6 epitope
    Use
    Tags
    BAP, HA, His6, T7, and TS site from proCCK
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    162777-plasmid-map-sequence-id-321882

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  19. pET24a-LaM4-1xTS

    Plasmid
    #162778
    Purpose
    Bacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to one tyrosine sulfation (TS) motif. LaM4-1xTS also contains a T7, HA, BAP and His6 epitope
    Depositor
    Martin Spiess
    Article
    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii
    Insert
    anti-mCherry nanobody fused to a TS site, T7, HA, BAP and His6 epitope
    Use
    Tags
    BAP, HA, His6, T7, and TS site from proCCK
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    162778-plasmid-map-sequence-id-321887

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  21. pET24a-LaM4-2xTS

    Plasmid
    #162779
    Purpose
    Bacterial expression of a functionalized anti-mCherry (LaM4) nanobody fused to two tyrosine sulfation (TS) motifs. LaM4-2xTS also contains a T7, HA, BAP and His6 epitope
    Depositor
    Martin Spiess
    Article
    Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii
    Insert
    anti-mCherry nanobody fused to two TS sites, T7, HA, BAP and His6 epitope
    Use
    Tags
    BAP, HA, His6, T7, and TS site from proCCK
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    162779-plasmid-map-sequence-id-321884

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  22. p37-2iDMD-LR

    Plasmid
    #88892
    Purpose
    37°C full-length DMD luc-red plasmid. Co-expresses human dystrophin, firefly luciferase and mCherry in mammalian cells. Plasmid carries phiC31 and Bxb1 attB sites and can be grown at 37°C.
    Depositor
    Michele Calos
    Article
    Farruggio et al Biotechnol J. 2017 Jan 31. doi: 10.1002/
    Inserts
    Dystrophin (DMD Human)
    luciferase
    mCherry
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    88892-plasmid-map-sequence-id-451853

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  23. p28-2iDMD-puro

    Plasmid
    #88895
    Purpose
    28°C full-length DMD luc-puro-red plasmid. Co-expresses human DMD, firefly luciferase, puroR and mCherry in mammalian cells. Plasmid carries phiC31 and Bxb1 attB sites, but *cannot* be grown at 37°C.
    Depositor
    Michele Calos
    Article
    Farruggio et al Biotechnol J. 2017 Jan 31. doi: 10.1002/
    Inserts
    Dystrophin (DMD Human)
    luciferase
    puromycin resistance enzyme
    mCherry
    Use
    Tags
    Expression
    Mammalian
    Mutation
    codon optimized
    Promoter
    Available since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    88895-plasmid-map-sequence-id-451851

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  24. Myc var1 K51/52 to NQ

    Plasmid
    #127287
    Purpose
    Expresses Myc with putative Ub site mutated
    Depositor
    Christine Milcarek
    Insert
    Myc (NM_001177352) (Myc Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    K51 and 52 to N and Q by Q5 mutagenesis
    Promoter
    CMV
    Available since
    Nov. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    127287-plasmid-map-sequence-id-250022

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  25. Myc var 1 K396/397 to NQ

    Plasmid
    #127286
    Purpose
    Expresses Myc with putative Ub site mutated
    Depositor
    Christine Milcarek
    Insert
    Myc (NM_001177352) (Myc Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    K396 and 397 to N and Q by Q5 mutagenesi
    Promoter
    CMV
    Available since
    July 15, 2019
    Availability
    Academic Institutions and Nonprofits only
    127286-plasmid-map-sequence-id-249993

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  26. pcDNA3-endo-Nb80

    Plasmid
    #205518
    Purpose
    Endosome-targeted Nb80 nanobody that binds and stabilizes β2AR active conformation, blocking Gαs coupling.
    Depositor
    Jin Zhang
    Article
    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:
    Insert
    endo-Nb80
    Use
    Tags
    2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    205518-plasmid-map-sequence-id-409484

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  27. pcDNA3-nuc-Nb80

    Plasmid
    #205519
    Purpose
    Nuclear-targeted Nb80 nanobody that binds and stabilizes β2AR active conformation, blocking Gαs coupling.
    Depositor
    Jin Zhang
    Article
    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:
    Insert
    nuc-Nb80
    Use
    Tags
    Nuclear localization signal (NLS) and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    205519-plasmid-map-sequence-id-409422

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  28. pcDNA3-endo-Nb37

    Plasmid
    #205514
    Purpose
    Endosome-targeted Nb37 nanobody that binds and stabilizes the active form of Gαs G-protein subunit.
    Depositor
    Jin Zhang
    Article
    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:
    Insert
    endo-Nb37
    Use
    Tags
    2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    205514-plasmid-map-sequence-id-409423

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  29. pcDNA3-nuc-Nb37

    Plasmid
    #205515
    Purpose
    Nuclear-targeted Nb37 nanobody that binds and stabilizes the active form of Gαs G-protein subunit.
    Depositor
    Jin Zhang
    Article
    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:
    Insert
    nuc-Nb37
    Use
    Tags
    Nuclear localization signal (NLS) and mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    205515-plasmid-map-sequence-id-409490

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  33. RFP-(SIM)3

    Plasmid
    #127148
    Purpose
    Mammalian expression plasmid for mCherry-(SIM)3 client to assess SUMO-SIM scaffolds.
    Depositor
    Michael Rosen
    Article
    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1
    Insert
    3 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (PIAS2 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    127148-plasmid-map-sequence-id-255288

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  34. pAS132

    Plasmid
    #211503
    Purpose
    AAV genome to express mCherry under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seq
    Depositor
    Randall Platt
    Article
    Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:
    Insert
    mCherry
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    211503-plasmid-map-sequence-id-417791

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  35. pH3mCHSH2

    Plasmid
    #101053
    Purpose
    This is a Cryptococcus neoformans mCherry expression vector with G418 drug selection marker and C. neoformans SH2 flanking sequences for genome integration.
    Depositor
    Jennifer Lodge
    Article
    Upadhya et al Fungal Genet Biol. 2017 Sep 1. pii: S108
    Insert
    Cryptococcus mCherry expression plasmid
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Cryptococcus Histone3 promoter
    Available since
    Oct. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    101053-plasmid-map-sequence-id-192942

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  36. pAAV-IV-3xFLAG/HA/NBL10

    Plasmid
    #110568
    Purpose
    Cre-dependently expresses large ribosomal subunit protein RPL10A with a 3xFLAG/HA tag
    Depositor
    Alexander Nectow, Jeffrey Friedman
    Insert
    3xFLAG/HA-tagged anti-GFP VHH
    Use
    AAV, Cre/Lox, and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    July 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    110568-plasmid-map-sequence-id-213334

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  38. pUT_mNF-Cas13d-Triplex

    Plasmid
    #227709
    Purpose
    LPUtopia matching RMCE donor plasmid with mCherry-P2A-RfxCas13d Negative-Feedback circuit. Include a 3' UTR Triplex motif as a template backbone for MONARCH 2.0 system.
    Depositor
    Gabor Balazsi
    Article
    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-
    Insert
    mCherry-P2A-RfxCas13d-Triplex
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV-d2i
    Available since
    Nov. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    227709-plasmid-map-sequence-id-450930

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  40. attB53_SNCB+_attB53

    Plasmid
    #183614
    Purpose
    RMCE vector to shuttle puromycin resistance, anti-GFP SynNotch receptor, tagBFP and TRE-mCherry cassettes into attP50-flanked landing pad (Addgene 183609). All cassettes on sense (+) strand.
    Depositor
    Sally Lowell
    Article
    Malaguti et al Development. 2022 May 26. pii: 275525. d
    Inserts
    LaG17 GFP nanobody SynNotch tTA
    TRE-mCherry-SV40pA
    tagBFP
    Use
    Rmce
    Tags
    Myc and human CD8a signal sequence
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    183614-plasmid-map-sequence-id-361603

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Showing: 1281 - 1320 of 1645 results