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Plasmid#222861PurposeThis plasmid codes for the OgeuIscB nickaseDepositorInsertOgeuIscB Nickase
UseCRISPRTags3X HA and Nucleoplasmin NLS and ITR2ExpressionMammalianMutationE193A for nickase mutationPromoterCMVAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-fDIO-ArgiNLS-AausFP1
Plasmid#220605PurposeFlp-dependent expression of a single-cell discriminating version of AausFP1 fluorescent protein.DepositorInsertAausFP1
UseAAVTagsArgiNLSExpressionMammalianMutationPromoterCAGAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-fDIO-ArgiNLS-mKate2
Plasmid#220607PurposeFlp-dependent expression of a single-cell discriminating version of mKate2 fluorescent protein.DepositorInsertmKate2
UseAAVTagsArgiNLSExpressionMammalianMutationPromoterCAGAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-fDIO-ArgiNLS-oScarlet
Plasmid#220606PurposeFlp-dependent expression of a single-cell discriminating version of oScarlet fluorescent protein.DepositorInsertoScarlet
UseAAVTagsArgiNLSExpressionMammalianMutationPromoterCAGAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-ArgiNLS-mVenus-Q69M(ME)
Plasmid#220597PurposeConstitutive expression of a single-cell discriminating version of mVenus-Q69M (ME) fluorescent protein.DepositorInsertmVenus-Q69M (ME)
UseAAVTagsArgiNLSExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FLEX-ArgiNLS-EGFP
Plasmid#220601PurposeCre-dependent expression of a single-cell discriminating version of EGFP fluorescent proteinDepositorInsertEGFP
UseAAV and Cre/LoxTagsArgiNLSExpressionMammalianMutationPromoterCAGAvailable sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro CAG eGFP NLS
Plasmid#170830PurposeDonor plasmid for knocking eGFP NLS into AAVS1 safe harbor locusDepositorInserteGFP
UseCRISPR, Synthetic Biology, and TALENTagsNLSExpressionMammalianMutationPromoterCAGAvailable sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-IDR4-mCh-Cry2WT-NLS
Plasmid#221920PurposeExpression of CBP IDR4 fragment fused to optogenetic protein mCh-Cry2WTDepositorInsertCBP IDR4 (aa 662-787) (CREBBP Human)
UseLentiviralTagsmCherryExpressionMammalianMutationinsertion of the IDR4 region (aa 662-787); insert…PromoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-CFID-mCh-Cry2WT-NLS
Plasmid#221923PurposeExpression of CBP CFID fragment fused to optogenetic protein mCh-Cry2WTDepositorInsertCBP CFID (aa 1892-2441) (CREBBP Human)
UseLentiviralTagsmCherryExpressionMammalianMutationinsertion of the CBP-FUS interaction domain (CFID…PromoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-IDR3-mCh-Cry2WT-NLS
Plasmid#221919PurposeExpression of CBP IDR3 fragment fused to optogenetic protein mCh-Cry2WTDepositorInsertCBP IDR3 (aa 441-587) (CREBBP Human)
UseLentiviralTagsmCherryExpressionMammalianMutationinsertion of the IDR3 region (aa 441 - 587); inse…PromoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-IDR7-mCh-Cry2WT-NLS
Plasmid#221922PurposeExpression of CBP IDR7 fragment fused to optogenetic protein mCh-Cry2WTDepositorInsertCBP IDR7 (aa 2109-2434) (CREBBP Human)
UseLentiviralTagsmCherryExpressionMammalianMutationinsertion of the IDR7 region (aa 2109 - 2434); in…PromoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-IDR6-mCh-Cry2WT-NLS
Plasmid#221921PurposeExpression of CBP IDR6 fragment fused to optogenetic protein mCh-Cry2WTDepositorInsertCBP IDR6 (aa 1856-2090) (CREBBP Human)
UseLentiviralTagsmCherryExpressionMammalianMutationinsertion of the IDR6 region (aa 1856-2090); inse…PromoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-IDR1-mCh-Cry2WT-NLS
Plasmid#221918PurposeExpression of CBP IDR1 fragment fused to optogenetic protein mCh-Cry2WTDepositorInsertCBP IDR1 (aa 72-175) (CREBBP Human)
UseLentiviralTagsmCherryExpressionMammalianMutationinsertion of the IDR1 region (aa 72 - 175); inser…PromoterAvailable sinceJuly 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
L1P137 - pAHP6-PhiC31-NLS-tUBQ1
Plasmid#219702PurposeLevel 1 pAHP6-PhiC31-NLS-tUBQ1, for xylem pole specific expression of PhiC31 integraseDepositorInsertpAHP6:PhiC31-NLS:tUBQ1
UseTagsExpressionPlantMutationPromoterAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-QUAS:NLS-mApple-he1.1:CFP
Plasmid#184814PurposeUsed to generate the Tg(QUAS:NLS-mApple; he1.1:CFP)c718 transgenic lineDepositorInsertQUAS:NLS-mApple-pA; he1.1:CFP
UseZebrafish tol2 transgenesisTagsNLS-mAppleExpressionMutationPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-QUAS:NLS-GFP-he1.1:CFP
Plasmid#184815PurposeUsed to generate the Tg(QUAS:NLS-GFP; he1.1:CFP)c682 transgenic lineDepositorInsertQUAS:NLS-GFP-pA; he1.1:CFP
UseZebrafish tol2 transgenesisTagsNLS-GFPExpressionMutationPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-2xflox-BRX-eGFP-NLS
Plasmid#217535PurposeAAV vector to drive the expression of eGFP in genetically defined cell populations under the control of the 4xBRE regulatory element of SMAD1 transcription factor and miniXon splicing casette in vivoDepositorArticleInsert4X BRE reporter and miniXon casette (Smad1 Mouse)
UseAAVTagsExpressionMammalianMutationPromoterBMP response element (BRE)Available sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-TET-NUbo-E3(48)-NLS-VSV
Plasmid#212746PurposeDoxycycline-inducible expression of NUbo-E3(48)-NLS-VSV in budding yeastDepositorInsertE3(48)
UseIntegrative vectorTagsNLS-VSV and NUboExpressionYeastMutationPromoterTetAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-TET-NUbo-E3(63)-NLS-VSV
Plasmid#212756PurposeDoxycycline-inducible expression of NUbo-E3(63)-NLS-VSV in budding yeastDepositorInsertE3(63)
UseIntegrative vectorTagsNLS-VSV and NUboExpressionYeastMutationPromoterTetAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-ADH-NUbo-E3(63)-NLS-VSV
Plasmid#212755PurposeConstitutive expression of NUbo-E3(63)-NLS-VSV in budding yeastDepositorInsertE3(63)
UseIntegrative vectorTagsNLS-VSV and NUboExpressionYeastMutationPromoterADH1Available sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
YIp128-ADH-NUbo-E3(48)-NLS-VSV
Plasmid#212745PurposeConstitutive expression of NUbo-E3(48)-NLS-VSV in budding yeastDepositorInsertE3(48)
UseIntegrative vectorTagsNLS-VSV and NUboExpressionYeastMutationPromoterADH1Available sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
A3Bctd-L7G-Cas9n-UGI-NLS
Plasmid#198886PurposeExpress A3Bctd-L7G BE3-based base editorDepositorInsertApolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal domain (APOBEC3B Human)
UseTagsNLSExpressionMammalianMutationLoop 7 of A3GPromoterCMVAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
A3Bctd-D314E-Cas9n-UGI-NLS
Plasmid#198887PurposeExpress A3Bctd-D314E BE3-based base editorDepositorInsertApolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal domain (APOBEC3B Human)
UseTagsNLSExpressionMammalianMutationD314EPromoterCMVAvailable sinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-NLS-MBP-rATOM(WDR5)
Plasmid#209710PurposeMammalian expression of r-ATOM biosensor for WDR5 with nuclear localization signalDepositorInsertNLS-MBP-rATOM(WDR5)
UseTagsNLS-MBPExpressionMammalianMutationPromoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
PET-B-HypaR-SpCas9-NLS-6xHis
Plasmid#207388PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertB-HypaR-SpCas9
UseCRISPRTags6xHisExpressionBacterialMutationR661A, N692A, M694A, Q695A, H698A, amino acids 10…PromoterT7Available sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
PET-HypaR-SpCas9-NLS-6xHis
Plasmid#207387PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertHypaR-SpCas9
UseCRISPRTags6xHisExpressionBacterialMutationR661A, N692A, M694A, Q695A, H698APromoterT7Available sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
PET-SpCas9-HF1-NLS-6xHis
Plasmid#207382PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertSpCas9-HF1
UseCRISPRTags6xHisExpressionBacterialMutationN497A, R661A, Q695A, Q926APromoterT7Available sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
PET-B-HiFi SpCas9-NLS-6xHis
Plasmid#207378PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertB-HiFi SpCas9
UseCRISPRTags6xHisExpressionBacterialMutationR691A, amino acids 1005-1013 replaced with two gl…PromoterT7Available sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
PET-Blackjack SpCas9-NLS-6xHis
Plasmid#207375PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInsertBlackjack SpCas9
UseCRISPRTags6xHisExpressionBacterialMutationamino acids 1005-1013 replaced with two glycinePromoterT7Available sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-safb∆sap-NLS-3XFLAG-V5
Plasmid#196091PurposeExpresses mouse SAFB lacking the n-terminal SAP domain in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tagDepositorInsertSAFB (Safb Mouse)
UseTagsC-terminal 3xFLAG and C-terminal V5ExpressionBacterial and MammalianMutationRemoved amino acids 31-65 (SAP domain) of mouse S…PromoterCAGGSAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-safb∆dd1-NLS-3XFLAG-V5
Plasmid#196092PurposeExpresses mouse SAFB lacking the first computationally called disordered domain in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tagDepositorInsertSAFB (Safb Mouse)
UseTagsC-terminal 3xFLAG and C-terminal V5ExpressionBacterial and MammalianMutationRemoved amino acids 96-285 (predicted disordered …PromoterCAGGSAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-safb∆rrm-NLS-3XFLAG-V5
Plasmid#196093PurposeExpresses mouse SAFB lacking the rrm domain in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tagDepositorInsertSAFB (Safb Mouse)
UseTagsC-terminal 3xFLAG and C-terminal V5ExpressionBacterial and MammalianMutationRemoved amino acids 428-506 (RRM) of mouse SAFB p…PromoterCAGGSAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-safb∆dd3-NLS-3XFLAG-V5
Plasmid#196094PurposeExpresses mouse SAFB lacking the third computationally called disordered domain in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tagDepositorInsertSAFB (Safb Mouse)
UseTagsC-terminal 3xFLAG and C-terminal V5ExpressionBacterial and MammalianMutationRemoved amino acids 638-925 (predicted disordered…PromoterCAGGSAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-SAFB-DD3-NLS-3XFLAG-V5
Plasmid#196095PurposeExpresses only the third computationally called disordered domain of mouse SAFB in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tagDepositorInsertSAFB (Safb Mouse)
UseTagsC-terminal 3xFLAG and C-terminal V5ExpressionBacterial and MammalianMutationContains only AA 619-925 (NLS and predicted disor…PromoterCAGGSAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT-ChemoG-NAD-NLS3x
Plasmid#193822PurposeCMV overexpression of ChemoG-NAD in the nucleus of mammalian cell linesDepositorInsertChemoG-NAD-NLS3x
UseTagsExpressionMammalianMutationEGFP:A206K, T225R; HT7: L271EPromoterCMVAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/TO-ChemoL-NAD-NLS3x
Plasmid#193828PurposeCMV overexpression of ChemoL-NAD in the nucleus of mammalian cell linesDepositorInsertChemoL-NAD-NLS3x
UseTagsExpressionMammalianMutationEGFP:A206K, T225R; HT7: L271EPromoterCMVAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-alphaCaMKII-dsRed-Express-3XNLS-pA
Plasmid#183802PurposeAAV vector to express nuclear localized dsRed-Express from CaMKIIa promoterDepositorInsertdsRed-Express
UseAAVTagsNLSExpressionMutationPromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
L0-I11 PhiC31-NLS GG1-GG4
Plasmid#195894PurposeLevel 0 PhiC31 inegrase NLS with GG1 GG4 Golden gate linkersDepositorInsertPhiC31 integrase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
L0-I12 PhiC31-NLS GG2-GG4
Plasmid#195895PurposeLevel 0 PhiC31 inegrase NLS with GG2 GG4 Golden gate linkersDepositorInsertPhiC31 integrase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-EF1a-ArgSen (-)P2A (-)NLS
Plasmid#184690PurposeLentiviral transfer plasmid for expression of ArgSen (-)P2A (-)NLSDepositorInsertArgSen (-)P2A (-)NLS
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only