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Plasmid#127760PurposeResearch the effect of gene orientation of transcriptionDepositorInsertmSpinach, MG RNA aptamer in tandem orientation
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEYRC-C1A
Plasmid#127752PurposeResearch the effect of gene orientation of transcriptionDepositorInsertRFP, CFP, Convergent Orientation
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS_KS_attB2_SA(2)_T2AGAL80
Plasmid#125219PurposeArtificial phase 2 exon to include a spliced T2A-GAL80 effector into a locus of interest through RMCE of MiMIC insertion in an intron between coding exons of a geneDepositorInsertGAL80
UseOtherTagsT2AExpressionMutationPromoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pISH-Vmn2r65
Plasmid#108083PurposePreparation of RNA probe for ISH of mouse Vmn2r65 geneDepositorInsertVmn2r65 (Vmn2r65 Mouse)
UseIn vitro transcriptionTagsExpressionMutationPromoterAvailable sinceMay 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pISH-Vmn2r76
Plasmid#108084PurposePreparation of RNA probe for ISH of mouse Vmn2r76 geneDepositorInsertVmn2r76 (Vmn2r76 Mouse)
UseIn vitro transcriptionTagsExpressionMutationPromoterAvailable sinceMay 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pISH-Vmn2r76-VR2
Plasmid#108085PurposePreparation of RNA probe for ISH of mouse Vmn2r76 geneDepositorInsertVmn2r76 (Vmn2r76 Mouse)
UseIn vitro transcriptionTagsExpressionMutationPromoterAvailable sinceMay 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pISH-Meis3
Plasmid#84312PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Meis3. Not for gene expression.DepositorInsertMeis3 (Meis3 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pILGFPB6
Plasmid#83557PurposeUsed to evaluate the expression output of GAL10 promoter with yEGFP used as the reporter gene.DepositorInsertGAL10 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterGAL10Available sinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pISH-Dmbx1
Plasmid#84308PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Dmbx1. Not for gene expression.DepositorInsertDmbx1 (Dmbx1 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceNov. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pISH-Barx2
Plasmid#84307PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Barx2. Not for gene expression.DepositorInsertBarx2 (Barx2 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceNov. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pBW215J105-Amp32C
Plasmid#78650PurposepSB3K3 carrying J105-30hrpR-32hrpS-t-hrpL-30gfp-tDepositorInsertJ105-30hrpR-32hrpS-t-hrpL-30gfp-t
UseSynthetic BiologyTagsExpressionMutationPromotersee insertAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBW212J114-Amp32C
Plasmid#78647PurposepSB3K3 carrying J114-30hrpR-32hrpS-t-hrpL-30gfp-tDepositorInsertJ114-30hrpR-32hrpS-t-hrpL-30gfp-t
UseSynthetic BiologyTagsExpressionMutationPromotersee insertAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
CAG-mCherry-RORbshRNA2
Plasmid#73984PurposeRORb shRNA2 (mir155 backbone) was inserted into the 3'UTR of mCherry.DepositorInsertshRNA that targets the mouse RORb gene
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
CAG-EGFP-RORbshRNA1
Plasmid#73985PurposeRORb shRNA1 (mir155 backbone) was inserted into the 3'UTR of EGFP.DepositorInsertshRNA that targets the mouse RORb gene
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
CAG-EGFP-RORbshRNA2
Plasmid#73986PurposeRORb shRNA2 (mir155 backbone) was inserted into the 3'UTR of EGFP.DepositorInsertshRNA that targets the mouse RORb gene
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
CAG-mCherry-Otx2shRNA2
Plasmid#73980PurposeOtx2 shRNA2 (mir155 backbone) was inserted into the 3'UTR of mCherry.DepositorInsertshRNA that targets the mouse Otx2 gene
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
CAG-mCherry-RORbshRNA1
Plasmid#73983PurposeRORb shRNA1 (mir155 backbone) was inserted into the 3'UTR of mCherry.DepositorInsertshRNA that targets the mouse RORb gene
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMay 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pISH-Pou6f1
Plasmid#74356PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Pou6f1. Not for gene expression.DepositorInsertPOU domain, class 6, transcription factor 1 (Pou6f1 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceApril 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pISH-Pou2f1
Plasmid#74355PurposeUse to synthesize riboprobes for in situ hybridization (ISH) probe for Pou2f1. Not for gene expression.DepositorInsertPOU domain, class 2, transcription factor 1 (Pou2f1 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceApril 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL3-RpS12-Gateway
Plasmid#71508PurposeflySTARR-seq validation vector with Gateway cassette downstream of the firefly luciferase gene and the RpS12 core promoterDepositorTypeEmpty backboneUseFlystarr-seq validation vectorTagsluciferaseExpressionInsectMutationPromoterAvailable sinceDec. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEYmRD3A
Plasmid#127756PurposeResearch the effect of gene orientation of transcriptionDepositorInsertmSpinach,RFP Divergent Orientation
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
AAV-PR rep/cap
Plasmid#197565Purposeencodes AAV-PR capsid that transduces pericytes and smooth muscle cells in mice after systemic deliveryDepositorInsertsAAV Rep genes
AAV9 VP1 Cap gene with PR insert
UseAAVTagsExpressionMutationcontains 7mer insert PRPPSTH between amino acids …PromoterAvailable sinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAVS1-tet-iCas9-BFP2
Plasmid#125519Purposeall-in-one-inducible Cas9DepositorInsertAAVS1-TET-ON 3G-SpCas9-P2A-BFP2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
Villinpromoter-blue-FlpOERT2
Plasmid#67278Purpose4hydroxytamoxifen inducible FlpO recombinase controlled by intestine specific promoter (Villin). FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon contextDepositorInsertmTQ2=mturquoise2 blue fluorescent gene
UseTagslinked to FlpO through an P2A ribosomal skipping …ExpressionMutationPromoterVillinAvailable sinceJuly 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
Donor_ANKRD1_KO_Puro
Plasmid#186667PurposeDonor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. eEF1a promoter, puromycin and polyA sequence is inserted between two homology arms.DepositorInsertANKRD1 KO Puromycin (ANKRD1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Donor_YTHDF2_KO_Hygro
Plasmid#186671PurposeDonor plasmid to endogenously knock out the YTHDF2 in Hek293 cells. Hygromycin and polyA sequence is inserted between two homology arms.DepositorInsertYTHDF2 KO Hygromycin (YTHDF2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
Donor_YTHDF2_KO_Puro
Plasmid#186670PurposeDonor plasmid to endogenously knock out the YTHDF2 in Hek293 cells. Puromycin and polyA sequence is inserted between two homology arms.DepositorInsertYTHDF2 KO Puromycin (YTHDF2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDH-EFIa-Id1-PGK-puro
Plasmid#107735PurposeExpresses Id1 in mammalian cellsDepositorInsertId1 (T Mouse)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-34: TNNI1-mEGFP
Plasmid#114411PurposeHomology arms and linker-mEGFP sequence for C-terminus tagging of human TNNI1, via excisable Cas9-excisable hPGK-mCherry selection cassetteDepositorInsertTNNI1 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (TNNI1 Human)
UseCRISPR; Donor templateTagslinker-mEGFPExpressionMammalianMutationPromoterAvailable sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
DENV4 Rluc Reporter GND mutant Replicon
Plasmid#118586PurposeFor use as a positive control for translation of the Rluc gene in the transfected cells and negative control for replication.DepositorInsertDENV4 Renilla luciferase gene (Rluc) reporter replicon containing replication-defective GND mutation in the NS5 polymerase gene.
UseYeast-e. col shuttle vector prs424TagsExpressionMutationThe conserved GDD in the NS5 polymerase gene muta…PromoterAvailable sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 (-) hYAP 1-2alpha
Plasmid#124143PurposeProtein expression for funtional studies of cell growth promotionDepositorInsertYAP1 (YAP1 Human)
UseUnspecifiedTagsExpressionMutationNone (Wild Type)PromoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX330-sgAAVS1
Plasmid#85802PurposeCas9/CRISPR vector for cut at human AAVS1 gene intron1DepositorInsertPPP1R12C (PPP1R12C Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHL-H1-ccdB-mEF1a-RiH
Plasmid#60601PurposeCloning vector for CRISPR-sgRNA (into the BamHI-EcoRI site), expresses RFP and hygromycin resistance gene.DepositorInsertsRed Fluorescent Protein
Hygromycin resistance gene
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
mARGAna (cassette 1)
Plasmid#191341PurposeSecond-generation mammalian acoustic reporter gene derived from AnabaenaDepositorInsertmARGAna (cassette 1)
UseTagsExpressionMammalianMutationPromoterTRE3GVAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
mARGAna (cassette 2)
Plasmid#191342PurposeSecond-generation mammalian acoustic reporter gene derived from AnabaenaDepositorInsertmARGAna (cassette 2)
UseTagsExpressionMammalianMutationPromoterTRE3GVAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
mARGAna (cassette 1, transient)
Plasmid#197588PurposeSecond-generation mammalian acoustic reporter gene derived from AnabaenaDepositorInsertmARGAna (cassette 1)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
mARGAna (cassette 2, transient)
Plasmid#197589PurposeSecond-generation mammalian acoustic reporter gene derived from AnabaenaDepositorInsertmARGAna (cassette 2)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4TO K164A H165A CoV-2 nsp1 3xFLAG
Plasmid#176060PurposeExpresses K164A K165A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG tag fusion to nsp1.DepositorInsertCoV-2 K164A H165A nsp1 (ORF1ab SARS-CoV-2)
UseTags3xFLAGExpressionMammalianMutationK164A and H165APromoterCMVAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4TO R124A K125A CoV-2 nsp1 3xFLAG
Plasmid#176059PurposeExpressing R124A K125A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG tag fusion to nsp1.DepositorInsertCoV-2 nsp1 R124A/K125A (ORF1ab SARS-CoV-2)
UseTags3xFLAGExpressionMammalianMutationR124A and K125APromoterCMVAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 (-) hYAP 1-2delta
Plasmid#124146PurposeProtein expression for funtional studies of cell growth promotionDepositorInsertYAP1 (YAP1 Human)
UseUnspecifiedTagsExpressionMutationNone (Wild Type)PromoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMH003_phiC31_neo-5xTetO-pEF-H2B-mCitrine-coreHS4-pRSV-H2B-mCherry
Plasmid#179431PurposeDual fluorescent reporter construct with single core HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integrationDepositorInsertTRE-cit-coreHS4-mch (SC)
UseTagsExpressionMammalianMutationPromoterpEF alpha, pRSVAvailable sinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 (-) hYAP 1-1delta
Plasmid#124142PurposeProtein expression for funtional studies of cell growth promotionDepositorInsertYAP1 (YAP1 Human)
UseUnspecifiedTagsExpressionMutationNone (Wild Type)PromoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 (-) hYAP 1-2gamma
Plasmid#124145PurposeProtein expression for funtional studies of cell growth promotionDepositorInsertYAP1 (YAP1 Human)
UseUnspecifiedTagsExpressionMutationNone (Wild Type)PromoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMH002_phiC31_neo-5xTetO-pEF-H2B-mCitrine-HS4-pRSV-H2B-mCherry
Plasmid#179430PurposeDual fluorescent reporter construct with single HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integrationDepositorInsertTRE-cit-HS4-mch (SH)
UseTagsExpressionMammalianMutationPromoterpEF alpha, pRSVAvailable sinceFeb. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Flag-Avi-ires-CHD5
Plasmid#68878PurposeTransient mammalian expression of CHD5 for BirA taggingDepositorInsertCHD5 (CHD5 Human)
UseTagsFLAG/AVIExpressionMammalianMutationPromoterCAGAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 (-) hYAP 1-1alpha
Plasmid#124139PurposeProtein expression for funtional studies of cell growth promotionDepositorInsertYAP1 (YAP1 Human)
UseUnspecifiedTagsExpressionMutationNone (Wild Type)PromoterAvailable sinceApril 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLY54
Plasmid#130923PurposeA CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA2B2 with sfgfp::ASV).DepositorInsertsdcas9
tetR
pspFΔHTH::λN22plus
sfgfp
UseSynthetic BiologyTagsASV tag and pspFΔHTH::λN22plusExpressionBacterialMutationMutations D10A and H840A on WT cas9. Synonymous m…PromoterAnderson promoter: J23106, PpspA-LEA2B2, and PtetAvailable sinceSept. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBABE (hygro) hYAP 1-1delta
Plasmid#124150PurposeProtein expression for funtional studies of cell growth promotionDepositorInsertYAP1 (YAP1 Human)
UseUnspecifiedTagsExpressionMutationNone (Wild Type)PromoterAvailable sinceApril 5, 2019AvailabilityAcademic Institutions and Nonprofits only