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Showing: 1601 - 1650 of 158755 results
  1. AAV-PR rep/cap

    Plasmid
    #197565
    Purpose
    encodes AAV-PR capsid that transduces pericytes and smooth muscle cells in mice after systemic delivery
    Depositor
    Inserts
    AAV Rep genes
    AAV9 VP1 Cap gene with PR insert
    Use
    AAV
    Tags
    Expression
    Mutation
    contains 7mer insert PRPPSTH between amino acids …
    Promoter
    Available since
    June 12, 2023
    Availability
    Academic Institutions and Nonprofits only
  2. Villinpromoter-blue-FlpOERT2

    Plasmid
    #67278
    Purpose
    4hydroxytamoxifen inducible FlpO recombinase controlled by intestine specific promoter (Villin). FlpO is linked to mTQ2 -a blue fluorescent protein. The gene cassette is in a SB transposon context
    Insert
    mTQ2=mturquoise2 blue fluorescent gene
    Use
    Tags
    linked to FlpO through an P2A ribosomal skipping …
    Expression
    Mutation
    Promoter
    Villin
    Available since
    July 14, 2015
    Availability
    Academic Institutions and Nonprofits only
  3. AICSDP-34: TNNI1-mEGFP

    Plasmid
    #114411
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human TNNI1, via excisable Cas9-excisable hPGK-mCherry selection cassette
    Insert
    TNNI1 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (TNNI1 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
  4. DENV4 Rluc Reporter GND mutant Replicon

    Plasmid
    #118586
    Purpose
    For use as a positive control for translation of the Rluc gene in the transfected cells and negative control for replication.
    Insert
    DENV4 Renilla luciferase gene (Rluc) reporter replicon containing replication-defective GND mutation in the NS5 polymerase gene.
    Use
    Yeast-e. col shuttle vector prs424
    Tags
    Expression
    Mutation
    The conserved GDD in the NS5 polymerase gene muta…
    Promoter
    Available since
    Aug. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
  5. pMH003_phiC31_neo-5xTetO-pEF-H2B-mCitrine-coreHS4-pRSV-H2B-mCherry

    Plasmid
    #179431
    Purpose
    Dual fluorescent reporter construct with single core HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration
    Depositor
    Insert
    TRE-cit-coreHS4-mch (SC)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pEF alpha, pRSV
    Available since
    May 24, 2022
    Availability
    Academic Institutions and Nonprofits only
  6. pMH002_phiC31_neo-5xTetO-pEF-H2B-mCitrine-HS4-pRSV-H2B-mCherry

    Plasmid
    #179430
    Purpose
    Dual fluorescent reporter construct with single HS4 insulator, upstream TRE (Tetracycline Response Element) recruitment site, phiC31 attB for integration
    Depositor
    Insert
    TRE-cit-HS4-mch (SH)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pEF alpha, pRSV
    Available since
    Feb. 28, 2022
    Availability
    Academic Institutions and Nonprofits only
  7. pLY54

    Plasmid
    #130923
    Purpose
    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA2B2 with sfgfp::ASV).
    Depositor
    Inserts
    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    Use
    Synthetic Biology
    Tags
    ASV tag and pspFΔHTH::λN22plus
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9. Synonymous m…
    Promoter
    Anderson promoter: J23106, PpspA-LEA2B2, and Ptet
    Available since
    Sept. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
Showing: 1601 - 1650 of 158755 results