We narrowed to 2,903 results for: BFP

1. pKLV2-U6gRNA5(JARID2_5-4)-PGKpuroBFP-W

   Plasmid

   #211966

   Purpose

   Express gRNA against JARID2 with puro and BFP

   Depositor

   Kosuke Yusa

   Article

   Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

   Insert

   sgRNA targeting JARID2 (JARID2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   Human U6 promoter

   Available Since

   Jan. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-pCaMKII-EBFP-tTAn

   Plasmid

   #172124

   Purpose

   Expression of EBFP and tTA-InteinN in CaMKII positive cells

   Depositor

   Chun Xu

   Article

   Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

   Insert

   pCaMKII, EBFP, tTAn-InteinN
   

   Use

   AAV

   Promoter

   EF1a

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMRX-IPU-mTagBFP2-Sec61B

   Plasmid

   #228136

   Purpose

   Expresses mTagBFP2-Sec61B in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Kurikawa et al PLoS Biol. 2024 Sep 17;22(9):e3002777. d

   Insert

   Sec61B (SEC61B Human)
   

   Use

   Retroviral

   Tags

   mTagBFP2

   Expression

   Mammalian

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTag-BFP-C-h-Rab4a-c-Myc

   Plasmid

   #79799

   Purpose

   Express TagBFP labeled human Rab4a (GTPase located on recycling endosomal membranes).

   Depositor

   James Johnson

   Article

   Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

   Insert

   Rab4a (RAB4A Human)
   

   Tags

   TagBFP and myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 22, 2016

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pME V5-mTagBFP2 (JDW 830)

   Plasmid

   #224484

   Purpose

   Gateway compatible middle entry clone containing V5 tagged mTagBFP2 (Cytosolic blue fluorescent reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   V5-mTagBFP2
   

   Use

   Gateway cloning

   Available Since

   Sept. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-TREtight-mTagBFP2-N2cG

   Plasmid

   #192838

   Purpose

   plasmid for making helper virus for monosynaptic tracing using CVS-N2c rabies virus; to be coinjected with pAAV-syn-FLEX-splitTVA-EGFP-tTA

   Depositor

   Ian Wickersham

   Insert

   mTagBFP2 and rabies virus CVS-N2c strain glycoprotein genes (N2cG)
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   TREtight

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair3

   Plasmid

   #98974

   Purpose

   Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 37bp 3' overhangs. TagBFP.

   Depositor

   Jan Karlseder

   Article

   Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

   Insert

   LMNA sgRNAs pair 3 and Cas9(D10A)
   

   Expression

   Mammalian

   Available Since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAGGS-mTagBFP2-T2A-iCre

   Plasmid

   #113837

   Purpose

   Monomeric TagBFP2 and iCre recombinase

   Depositor

   Mario Capecchi

   Insert

   mTagBFP2-T2A-iCre
   

   Expression

   Mammalian

   Available Since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. TagBFP2-C1-SACM1LdeltaTMD-C389S-Fis1tail

   Plasmid

   #220079

   Purpose

   SAC1 with a C389S mutation in it with the TMD deleted and replaced with the Fis1tail to replace the TMD and target the mitochondria instead of the ER with a Blue Fluorophore

   Depositor

   Gerry Hammond

   Article

   Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

   Insert

   SACM1l-C389S-Fis1tail (SACM1L Human)
   

   Expression

   Mammalian

   Mutation

   C389SdeltaTMD

   Available Since

   May 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pME-nCas9n-P2A-TagBFP2-pA

    Plasmid

    #176026

    Purpose

    Multisite gateway vector for expression of nCas9n with blue reporter. Parton lab clone ENT

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    nCas9n-P2A-TagBFP2
    

    Use

    Gateway entry clone

    Available Since

    Oct. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFA6a-link-yomTagBFP-SpHis5

    Plasmid

    #44838

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mTagBFP with SpHis5 selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomTagBFP
    

    Expression

    Yeast

    Available Since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-hSyn1-TagBFP2/CAAX

    Plasmid

    #197590

    Purpose

    Lentiviral transfer vector for the neuronal expression of membrane-bound TagBFP2 (good visualization of neuronal processes)

    Depositor

    Patrik Verstreken

    Insert

    mTagBFP2-CAAX
    

    Use

    Lentiviral

    Tags

    CAAX

    Promoter

    hSynI

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLIX403-EBFP2-ccdB-Puro

    Plasmid

    #158552

    Purpose

    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EBFP2

    Expression

    Mammalian

    Available Since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pVH231-Tier1-PmPGK1-mTagBFP2

    Plasmid

    #169520

    Purpose

    Tier-1 vector encoding PmPGK1-driven mTagBFP2 expression (PmPGK1-mTagBFP2-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    PPGK-driven monomeric Tag blue fluorescent protein 2
    

    Expression

    Mammalian

    Promoter

    PPGK

    Available Since

    June 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJL2-mGold-P2A-EBFP2

    Plasmid

    #157998

    Purpose

    Co-expression of mGold and EBFP2 in mammalian cells.

    Depositor

    Francois St-Pierre

    Article

    Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

    Insert

    mGold-P2A-EBFP2
    

    Expression

    Mammalian

    Mutation

    mGold is mVenus with L46F;T63S mutations

    Promoter

    CMV

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSLQ5135: pCAG-BFP-GuideArray

    Plasmid

    #210606

    Purpose

    Guide array for hyperdCas12a-based transcriptional activator with AAAT synSeparator

    Depositor

    Stanley Qi

    Article

    Liu et al Nat Commun. 2023 Oct 18;14(1):6575. doi:

    Insert

    pCAG-BFP-GuideArray
    

    Use

    CRISPR

    Promoter

    CAG

    Available Since

    Dec. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRepair-MPP8_mAID-P2A-BFP

    Plasmid

    #175036

    Purpose

    Homology Directed Repair construct for C-terminal tagging of mMPP8 with mAID using CRISPR/Cas9

    Depositor

    Kristian Helin

    Article

    Muller et al Nat Commun. 2021 May 24;12(1):3034. doi:

    Insert

    mAID-P2A-BFP
    

    Use

    Hdr donor template

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDONOR-tagBFP-PSM-EGFP

    Plasmid

    #100603

    Purpose

    Donor scaffold with EGFP positive selection module, and tagBFP negative selection module

    Depositor

    Jens Schwamborn

    Article

    Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    EGFP-T2A-Puro and tagBFP

    Available Since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pUC-hU6-crScaffold_SV40-BFP

    Plasmid

    #224860

    Purpose

    crRNA scaffold for RfxCas13d expressed from hU6 promoter and reporter BFP protein expressed from SV40 promoter

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBS-KS-attB1-2-PT-SA-SD-2-mTagBFP2(11x7)

    Plasmid

    #172066

    Purpose

    Protein trap plasmid for inserting mTagBFP2(11x7) into a phase 2 coding intron

    Depositor

    Daichi Kamiyama

    Article

    Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

    Insert

    mTagBFP2(11x7)
    

    Expression

    Insect

    Available Since

    Oct. 20, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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