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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,212 results for: Ncl

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Showing: 641 - 660 of 1212 results

  5. pDH5

    Plasmid
    #83775
    Purpose
    Useful for tagging yeast proteins with C-terminal YFP tag; his selection
    Depositor
    Eric Muller
    Article
    Hailey et al Methods Enzymol. 2002;351:34-49.
    Insert
    YFP
    Tags
    YFP
    Expression
    Yeast
    Mutation
    S65G, V68L, Q69K, S72A, T203Y. Also includes Q80R…
    Available Since
    Oct. 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    83775-plasmid-map-sequence-id-159997

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  6. Gli3ZF in AINGpCAGGS

    Plasmid
    #121972
    Purpose
    Gli3 zinc fingers
    Depositor
    James Briscoe
    Insert
    Gli3ZF (GLI3 Human)
    Use
    Chick electroporation
    Tags
    5xmyc
    Expression
    Mammalian
    Mutation
    N terminus and C terminus deletion only nt 1411-1…
    Available Since
    April 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    121972-plasmid-map-sequence-id-241924

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  8. AAA

    Plasmid
    #78487
    Purpose
    2135nt RNA encoded by chimaeric gene cloned in a pSP65A vector that included about 65 dT residues for transcription of a poly(A) tail
    Depositor
    Allan Jacobson
    Article
    Amrani et al Nature. 2008 Jun 26;453(7199):1276-80. d
    Insert
    CAN1-LUC
    Expression
    Bacterial
    Mutation
    CAN1 contains no stop codon
    Available Since
    Aug. 31, 2016
    Availability
    Academic Institutions and Nonprofits only

  13. pLSLZ.D

    Plasmid
    #51496
    Purpose
    pLSL with Zif268:FokI coding sequence followed by us:NPTII repair template , Rep is not included on this plasmid
    Depositor
    Daniel Voytas
    Article
    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:
    Inserts
    Zif268:FokI
    us:NPTII repair template
    Use
    Plant t-dna plasmid
    Tags
    SV40 NLS
    Promoter
    virion-sense LIR and 2x35S
    Available Since
    May 2, 2014
    Availability
    Academic Institutions and Nonprofits only
    51496-plasmid-map-sequence-id-78596

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  15. pET28-RPL3-72~197-mEGFP(A206K)-TwStrep

    Plasmid
    #208625
    Purpose
    for E. coli expression of truncated human RPL3 (ENST00000216146), aa72~197, fused with mEGFP(A206K) and Twin Strep tag
    Depositor
    Quanfu Ma
    Insert
    human RPL3 (ENST00000216146) aa 72~197 (RPL3 Human)
    Tags
    6xHis, Twin Strep, and mEGFP(A206K)
    Expression
    Bacterial
    Mutation
    only aa 72~197, other residues not included
    Promoter
    T7
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    208625-plasmid-map-sequence-id-433638

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  17. pcDNA3.1-ARL16-His6

    Plasmid
    #216484
    Purpose
    Used for exogenous expression of human ARL16, short form, in mammalian cells. The encoded protein contains a C-terminal, glycine rich linker and His6 tag
    Depositor
    Richard Kahn
    Insert
    ADP-ribosylation factor like 16 (ARL16 Human)
    Tags
    includes GSGSGSHHHHHH (His6) tag at C-terminus
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    216484-plasmid-map-sequence-id-426903

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  18. pcDNA3.1-msEGFP1.8

    Plasmid
    #208619
    Purpose
    Mammalian expression of a EGFP variant. Mutations were adopted from (Addgene#135301, PMC7508230), named msEGFP1.8 because N-ter and C-ter features of that in msGFP2 (PMC7508230) not included.
    Depositor
    Quanfu Ma
    Insert
    msEGFP1.8
    Expression
    Mammalian
    Mutation
    (compared with pEGFP-C1, protein level) S30R, Y39…
    Promoter
    CMV
    Available Since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208619-plasmid-map-sequence-id-418315

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  19. pET24a MDHM1_ARATH

    Plasmid
    #204236
    Purpose
    Bacterial expression of Arabidopsis mitochondrial malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).
    Depositor
    Malate dehydrogenase CUREs Community (MCC)
    Insert
    MDHM1_ARATH (mMDH1 Mustard Weed)
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Does done include the first 52 residues that are …
    Promoter
    Lac
    Available Since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    204236-plasmid-map-sequence-id-413347

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Showing: 641 - 660 of 1212 results