We narrowed to 1,221 results for: POLI

1. AAV-Syn-NS1

   Plasmid

   #175276

   Purpose

   AAV vector mediating bicistronic expression of NS1 gene of YFV-17D and dTomato with NLS

   Depositor

   Wei Xu

   Article

   Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

   Insert

   Nonstructural protein 1 (NS1) of YFV-17D; NLS-dTomato (POLY Yellow fever virus strain 17D)
   

   Use

   AAV

   Tags

   NLS-dTomato (P2A cleavage)

   Promoter

   Synapsin

   Available Since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28_6xHis-PARP1delZn2delBRCT

   Plasmid

   #173939

   Purpose

   Bacterial expression of PARP1 lacking Zn2 and BRCT domains

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1delZn2delBRCT (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Deletion of residues 97-213 and 374-520; V762A

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28_6xHis-PARP1-E988K

   Plasmid

   #173945

   Purpose

   Bacterial expression of inactive PARP1 mutant (E988K attenuates catalytic and PAR-branching activity of PARP1)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-E988K (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   V762A, E988K

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28_6xHis-PARP1-CATdeltaHD

   Plasmid

   #174811

   Purpose

   Bacterial expression of a hyperactive PARP1 CAT lacking the autoinhibitory HD subdomain

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-CATdeltaHD (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Residues 678-787 replaced by eight-residue linker…

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-TRE-NS1-dTomato

   Plasmid

   #175278

   Purpose

   AAV vector mediating inducible bicistronic expression of NS1 gene of YFV-17D and dTomato

   Depositor

   Wei Xu

   Article

   Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

   Insert

   NS1; dTomato (POLY Yellow fever virus strain 17D)
   

   Use

   AAV

   Tags

   dTomato (from bidirectional promoter)

   Promoter

   bidirectional TRE promoter

   Available Since

   Dec. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-DIO-NS1

   Plasmid

   #175273

   Purpose

   AAV vector mediating Cre-depenent expression of NS1 gene of YFV-17D

   Depositor

   Wei Xu

   Article

   Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

   Insert

   Nonstructural protein 1 (NS1) of YFV-17D (POLY Yellow fever virus strain 17D)
   

   Use

   AAV

   Promoter

   Synapsin

   Available Since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Neurog2

   Plasmid

   #99694

   Purpose

   Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Actc1, vector allows for strong activation of mouse Actc1, can be packaged and delivered as AAV

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9 and gRNA targeting Actc1 (gRNA: GGTATATAAGGGGTTTTAAG) (Actc1 Mouse, Synthetic)
   

   Use

   AAV and CRISPR

   Tags

   VPR mini

   Mutation

   dead Cas9

   Available Since

   Jan. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28_6xHis-PARP1-CAT-L713F

   Plasmid

   #173944

   Purpose

   Bacterial expression of isolated PARP1 CAT domain containing L713F gain-of-function mutation

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-CAT-L713F (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   L713F, V762A

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28_6xHis-PARP1-L713F

   Plasmid

   #174794

   Purpose

   Bacterial expression of a hyperactive PARP1 mutant (L713F destabilizes the autoinhibitory HD subdomain)

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1-L713F (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   L713F, V762A

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28_6xHis-PARP1-C125G

    Plasmid

    #174792

    Purpose

    Bacterial expression of a less active PARP1 mutant (C125G reduces affinity for single-strand DNA break)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-C125G (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    C125G, V762A

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28_6xHis-PARP1-Y986H

    Plasmid

    #174801

    Purpose

    Bacterial expression of a PARP1 mutant that produces highly branched but short PAR chains overall

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-Y986H (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Y986H, V762A

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28_6xHis-PARP1-Y986S

    Plasmid

    #174802

    Purpose

    Bacterial expression of a PARP1 mutant that produces mainly short PAR oligomers

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-Y986S (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Y986H, V762A

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28_6xHis-PARP1-G972R

    Plasmid

    #174800

    Purpose

    Bacterial expression of a PARP1 mutant that produces less PAR oligomers overall

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-G972R (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    G972R, V762A

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28_6xHis-PARP1delWGR-L713F

    Plasmid

    #173941

    Purpose

    Bacterial expression of PARP1 lacking WGR domain but containing L713F gain-of-function mutation

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1delWGR-L713F (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Deletion of residues 521-660; L713F, V762A

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28_6xHis-PARP1-A774L

    Plasmid

    #174796

    Purpose

    Bacterial expression of a hyperactive PARP1 mutant (A774L destabilizes the autoinhibitory HD subdomain)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-A774L (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    A774L, V762A

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28_6xHis-PARP1-W318R

    Plasmid

    #174793

    Purpose

    Bacterial expression of an inactive PARP1 (W318R disrupts interdomain communication and HD subdomain unfolding)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-W318R (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    W318, V762A

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28_6xHis-PARP1-A762V

    Plasmid

    #174795

    Purpose

    Bacterial expression of wild type PARP1 mutant (A762V is reversion of the common V762A polymorphism)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-A762V (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    A762V

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28_6xHis-PARP1delZn1-L713F

    Plasmid

    #173937

    Purpose

    Bacterial expression of PARP1 lacking Zn1 domain but containing L713F gain-of-function mutation

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1delZn1-L713F (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Deletion of residues 1-96; L713F, V762A

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28_6xHis-PARP1delZn3-L713F

    Plasmid

    #173934

    Purpose

    Bacterial expression of PARP1 lacking Zn3 domain but containing L713F gain-of-function mutation

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1delZn3-L713F (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Deletion of residues 214-373; L713F, V762A

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28_6xHis-PARP1-G871L

    Plasmid

    #174798

    Purpose

    Bacterial expression of a hyperactive PARP1 mutant (G871L destabilizes the autoinhibitory HD subdomain)

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-G871L (PARP1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    G871L, V762A

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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