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pEF mito-cGFP (matrix)

Plasmid

#188904

Purpose

Expresses mitochondrial GFP

Depositor

Edwin Chapman

Article

Vevea et al J Neurosci. 2023 May 10;43(19):3421-3438

Insert

mitochondrial GFP

Use

Tags

Mitochondrial leader sequence: MSVLTPLLLRGLTGSARR…

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-Cas13d-T2A-Citrine

Plasmid

#224565

Purpose

Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), Cas13d, and Citrine reporter.

Depositor

Erica Hutchins

Article

Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

Insert

Cas13d-T2A-Citrine

Use

CRISPR

Tags

T2A-Citrine

Expression

Mutation

Promoter

Available since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pTriEx-RhoA-wt_mScarlet-i_SGFP2

Plasmid

#85071

Purpose

Green/red FRET biosensor to monitor RhoA nucleotide loading state and activity

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

RhoA Biosensor wildtype with mScarlet-i and SGFP2 (RHOA Human)

Use

Tags

6xHis and mScarlet-i and SGFP2

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pTriEx-NTOM20-mVenus-Zdk2

Plasmid

#81011

Purpose

Expression of Zdk2 anchored to mitochondria

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

Zdk2

Use

Tags

6x His tag, FLAG, NTOM20, and mVenus

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

Available since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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Plasmid L5 attB:Psmyc triple red

Plasmid

#169413

Purpose

Chromosomally-Integrated Fluorescent Mycobacterium tuberculosis Reporter Constructs for triple red

Depositor

Clifton Barry III

Article

Kolbe et al Front Microbiol. 2020 Dec 9;11:591866. d

Inserts

mScarlet proper
COmScarlet
mRuby3

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pMOS017: ratiometric pHluorin (cytosolic)

Plasmid

#163052

Purpose

ratiometric pHluorin (cytosolic)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

ratiometric pHluorin

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pKH3-TRIM28-C65A/C68A

Plasmid

#92199

Purpose

Mammalian overexpression vector for TRIM28; catalytically inactive (mutated on C65A and C68A)

Depositor

Huda Zoghbi

Article

Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

Insert

Tif1b (TRIM28 Human, Synthetic)

Use

Tags

HA

Expression

Mammalian

Mutation

Changed Cysteines 65 and 68 to Alanines.

Promoter

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pFastBac1-OSF-TRIM5alpha(human)

Plasmid

#79034

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

OSF-TRIM5alpha(human) (TRIM5 Human)

Use

Tags

OneSTrEP-FLAG

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

March 3, 2017

Availability

Academic Institutions and Nonprofits only

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C1-mCitrine-FKBP-EZRabd(t567D)

Plasmid

#155227

Purpose

Acute increase in MPA when used with FRB-FKBP dimerizaton system upon addition with Rapamycin

Depositor

Tobias Meyer

Article

Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

Insert

mCitrine fused to FKBP and the actin BD of Ezrin (T567D), aa552-586 for use with FRB and rapamycin induced dimerization system

Use

Tags

mCitrine

Expression

Mammalian

Mutation

Changed Thr 567 to Asp in original Ezrin

Promoter

Available since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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LMPd Ametrine Got2 shRNA#3

Plasmid

#220593

Purpose

Retroviral vector with Ametrine marker for expression shRNA with an "UltramiR" microRNA scaffold

Depositor

Russell Jones

Article

Ma et al bioRxiv [Preprint]. 2023 Jun 11:2023.06.

Insert

Got2 shRNA (Got2 Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-mAmetrine1.1-WPRE

Plasmid

#193340

Purpose

AAV-mediated expression of mAmetrine1.1 under the TRE promoter in mammalian cells

Depositor

Takeshi Imai

Article

Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

Insert

mAmetrine1.1

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pDestTol2CG2-mnx1:ReaChR-citrine

Plasmid

#169046

Purpose

Destination transgenesis vector for expressing ReaChR fused with citrine in zebrafish spinal motor neurons using 3 tandem repeats of the mnx1 enhancer.

Depositor

Paola Arlotta

Article

Harris et al Dev Cell. 2020 Jun 8;53(5):577-588.e7. d

Insert

ReaChR-Citrine

Use

Tags

Citrine

Expression

Mammalian

Mutation

Promoter

3xMnx1

Available since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-mtagBFP-2A STINGHAQ

Plasmid

#102587

Purpose

Lentivector to express STING HAQ and mTagBFP

Depositor

Nicolas Manel

Article

Cerboni et al J Exp Med. 2017 Jun 5;214(6):1769-1785.

Insert

STING (STING1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

R71H-G230A-R293Q (HAQ)

Promoter

SFFV

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LOV2 I427T

Plasmid

#81035

Purpose

Mammalian expression of LOV I427T, a mutant with super short relaxation time

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV I427T

Use

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

I427T

Promoter

Available since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pTRIP-SFFV-Hygro-2A-TMRPSS2

Plasmid

#170390

Purpose

Generation of stable lines to enhance SARS-CoV-2 infectivity in ACE2 expressing cells

Depositor

Nir Hacohen

Article

Filbin et al Cell Rep Med. 2021 May 18;2(5):100287. d

Insert

TMPRSS2 (TMPRSS2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

SFFV

Available since

June 23, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-FLAG-dTomato-centrin-1

Plasmid

#73332

Purpose

Lentiviral vector encoding centrin-1 with an N-terminal FLAG and dTomato tag

Depositor

Manuel Thery

Insert

centrin-1 (CETN1 Human)

Use

Lentiviral

Tags

FLAG and dTomato

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pTriEx-NLyn-Venus-Zdk1

Plasmid

#81042

Purpose

mammalian expression of plasma-membrane anchored Zdk1

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

Zdk1

Use

Tags

6x His tag, FLAG, NLyn, and mVenus

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

Available since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-PA-Rac1-T17N

Plasmid

#22029

Purpose

Expression of photoactivatable Rac1 (dominant-negative mutant).

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

PA-Rac1-T17N (RAC1 Human, Avena sativa (oat))

Use

Tags

6xHis and mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

LOV(Leu404-Leu546). Rac1 starts at Ile4 and conta…

Promoter

CMV, p10

Available since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only

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AAV-flex-oChIEF-citrine

Plasmid

#50973

Purpose

flexed oChIEF-citrine construct in an AAV transfer vector containing AAV2 ITR

Depositor

Roger Tsien

Article

Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

Insert

ChIEF-citrine

Use

AAV

Tags

citrine

Expression

Mutation

inverted and inserted between two lox sites

Promoter

human synapsin promoter

Available since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pMOS018: ratiometric pHluorin (mitchondrial)

Plasmid

#163053

Purpose

ratiometric pHluorin (mitchondrial)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

ratiometric pHluorin

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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