We narrowed to 12,119 results for: cel.2

1. pDY2262 hACTB atgRNA Paired Guide 2

   Plasmid

   #220992

   Purpose

   hACTB atgRNA

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Fell et al Nat Protoc. 2024 Dec 15. doi: 10.1038/s4

   Insert

   ACTB atgRNA (ACTB Human)
   

   Use

   CRISPR

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCDNA3-SREBP-2-GFP (A1134-1135)

   Plasmid

   #204203

   Purpose

   Mammalian expression of SREBP-2

   Depositor

   Allan Weissman

   Article

   Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

   Insert

   SREBP-2 (SREBF2 Human)
   

   Tags

   T7x3 and eGFP

   Expression

   Mammalian

   Mutation

   Gly to Ala 1134, 1135

   Available Since

   April 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLKO.1-Teton-puro-shPP1C #2

   Plasmid

   #198763

   Purpose

   conditional knockdown of PP1C

   Depositor

   Hyonchol Jang

   Article

   Kim et al Cancers (Basel). 2020 Sep 11;12(9):2601.

   Insert

   shPP1C #2 (PPP1CC Human)
   

   Expression

   Mammalian

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. peSpCas9(1.1)-2×sgRNA (empty, donor)

   Plasmid

   #80768

   Purpose

   All-in-one vector for CRISPR/Cas9-mediated homology-independent knock-in system. The plasmid contains eSpCas9(1.1) and two sgRNA expression cassettes. The first gRNA cloning site is empty.

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP-Bcl-2-G128L-A131L-T132L

   Plasmid

   #220304

   Purpose

   mammalian expression of Bcl-2-G128L-A131L-T132L tagged with GFP

   Depositor

   Xinlai Cheng

   Article

   Wang et al Cell Reports Physical Science, Volume 5,

   Insert

   Bcl2 (BCL2 Human)
   

   Tags

   Emerald GFP

   Expression

   Mammalian

   Mutation

   G128L-A131L-T132L

   Promoter

   CMV

   Available Since

   June 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKLV2-U6gRNA5(Control(2))-PGKpuro2AmCherry-W

   Plasmid

   #210613

   Purpose

   Lentiviral vector expressing gRNA targeting a control region at the CXCR4 locus

   Depositor

   Kosuke Yusa

   Article

   Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

   Insert

   Control(2)
   

   Use

   Lentiviral

   Available Since

   Jan. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-30a-NSP14-SARS-CoV-2

   Plasmid

   #236423

   Purpose

   bacterial protein expression of NSP14

   Depositor

   Michele Pagano

   Article

   Rona et al Cell Death Differ. 2022 Feb;29(2):285-29

   Insert

   NSP14 (ORF1ab SARS-CoV-2)
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   May 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. MAC-C Sars-CoV-2 NSP12_P314L

   Plasmid

   #194813

   Purpose

   Sars-CoV-2 NSP12_P314L in MAC-C destination vector

   Depositor

   Markku Varjosalo

   Article

   Varjosalo et al 10 May 2024, Research Square

   Insert

   NSP12_P314L (ORF1ab Sars-CoV-2)
   

   Tags

   MAC-C

   Expression

   Mammalian

   Mutation

   P314L

   Available Since

   May 16, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pLEX307-SARS-CoV-2-3CL-C145A

   Plasmid

   #160279

   Purpose

   Allows for constituitive mammalian expression of the SARS-CoV-2 3CL protease with C145A mutation via transfection or lentivirus

   Depositor

   Alejandro Chavez

   Article

   Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

   Insert

   3CL Protease
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   C145A

   Promoter

   EF1a

   Available Since

   Oct. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-30a-NSP10-SARS-CoV-2

    Plasmid

    #236426

    Purpose

    bacterial protein expression of NSP10

    Depositor

    Michele Pagano

    Article

    Rona et al Cell Death Differ. 2022 Feb;29(2):285-29

    Insert

    NSP10 (ORF1ab SARS-CoV-2)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. MAC-C Sars-CoV-2 N_D3L

    Plasmid

    #194806

    Purpose

    Sars-CoV-2 N_D3L in MAC-C destination vector

    Depositor

    Markku Varjosalo

    Article

    Varjosalo et al 10 May 2024, Research Square

    Insert

    N_N_D3L (N Sars-CoV-2)
    

    Tags

    MAC-C

    Expression

    Mammalian

    Mutation

    N_D3L

    Available Since

    May 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. MAC-C Sars-CoV-2 N_D63G

    Plasmid

    #194807

    Purpose

    Sars-CoV-2 N_D63G in MAC-C destination vector

    Depositor

    Markku Varjosalo

    Article

    Varjosalo et al 10 May 2024, Research Square

    Insert

    N_D63G (N Sars-CoV-2)
    

    Tags

    MAC-C

    Expression

    Mammalian

    Mutation

    D63G

    Available Since

    May 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. MAC-C Sars-CoV-2 N_R203M

    Plasmid

    #194810

    Purpose

    Sars-CoV-2 N_R203M in MAC-C destination vector

    Depositor

    Markku Varjosalo

    Article

    Varjosalo et al 10 May 2024, Research Square

    Insert

    N_R203M (N Sars-CoV-2)
    

    Tags

    MAC-C

    Expression

    Mammalian

    Mutation

    R203M

    Available Since

    May 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. MAC-C Sars-CoV-2 N_S235F

    Plasmid

    #194811

    Purpose

    Sars-CoV-2 N_S235F in MAC-C destination vector

    Depositor

    Markku Varjosalo

    Article

    Varjosalo et al 10 May 2024, Research Square

    Insert

    N_S235F (N Sars-CoV-2)
    

    Tags

    MAC-C

    Expression

    Mammalian

    Mutation

    S235F

    Available Since

    May 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. HA-C Sars-CoV-2 N_T205I

    Plasmid

    #194812

    Purpose

    Sars-CoV-2 N_T205I in MAC-C destination vector

    Depositor

    Markku Varjosalo

    Article

    Varjosalo et al 10 May 2024, Research Square

    Insert

    N_T205I (N Sars-CoV-2)
    

    Tags

    MAC-C

    Expression

    Mammalian

    Mutation

    T205I

    Available Since

    May 16, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pKLV2-U6gRNA5(ZNF398_5-2)-PGKpuroBFP-W

    Plasmid

    #211998

    Purpose

    Express gRNA against ZNF398 with puro and BFP

    Depositor

    Kosuke Yusa

    Article

    Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

    Insert

    sgRNA targeting ZNF398 (ZNF398 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    Human U6 promoter

    Available Since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pKLV2-U6gRNA5(AAVS1-2)-PGKpuroBFP-W

    Plasmid

    #211955

    Purpose

    Express safe-cutter control gRNA with puro and BFP

    Depositor

    Kosuke Yusa

    Article

    Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

    Insert

    safe-cutter control sgRNA_AAVS1-2 (AAVS1 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    Human U6 promoter

    Available Since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLKO.1 puro CXCR4 siRNA-2

    Plasmid

    #12272

    Depositor

    Bob Weinberg

    Article

    Orimo et al Cell. 2005 May 6. 121(3):335-48.

    Insert

    CXCR4 siRNA (CXCR4 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    July 20, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBIG1a_3xFlag-6His-nsp15 (SARS-CoV-2)

    Plasmid

    #169166

    Purpose

    Baculoviral transfer vector for the expression of SARS-CoV-2 nsp15 in insect cells

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2465-2479

    Insert

    3xFlag-6His-nsp15 (ORF1ab SARS-CoV-2, Synthetic)
    

    Tags

    3xFlag-6His

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-bio-myc-mCE 2-210

    Plasmid

    #112715

    Purpose

    Expresses N-terminally bio-myc-tagged mouse CE/Rngtt 2-210 in mammalian cells

    Depositor

    Daniel Schoenberg

    Article

    Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

    Insert

    Rngtt (Rngtt Mouse)
    

    Tags

    biotinylation signal peptide (Tagwerker et al. 20…

    Expression

    Mammalian

    Mutation

    Silent mutation at Arg 132 (CGT to CGC), deleted …

    Promoter

    CMV

    Available Since

    Oct. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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