We narrowed to 4,062 results for: p2a

1. pAAV2-GCaMP5g-P2A-PAmCherry

   Plasmid

   #133411

   Purpose

   Fluorescent reporter for calcium imaging and photoactivation.

   Depositor

   Tim Holy

   Article

   Lee et al Science. 2019 Dec 13;366(6471):1384-1389

   Insert

   GCaMP5g-P2A-PAmCherry
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-xBE4Gam-P2A-Puro

   Plasmid

   #112676

   Purpose

   Lentivirus for constitutive expression of xCas9(3.7)-BE4Gam in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   xCas9(3.7)-BE4Gam
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

   Promoter

   EF1s

   Available since

   July 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA4TO-24xGCN4_v1-P2A-sfGFP

   Plasmid

   #61057

   Purpose

   sfGFP and the 24xGCN4_v1 peptide array are expressed from the same transcript, separated by a P2A site

   Depositor

   Ron Vale

   Article

   Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

   Insert

   24xGCN4_v1 and sfGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

4. CMV:eGFP-p2A-HA-GPR151(p.Arg95Ter)

   Plasmid

   #190134

   Purpose

   expresses eGFP-p2A-HA-GPR151 (pArg95Ter variant) in mammalian cells

   Depositor

   Joshua Knowles

   Article

   Bielczyk-Maczynska et al Nat Commun. 2022 Dec 1;13(1):7408. doi:

   Insert

   eGFP-p2A-HA-GPR151(p.Arg95Ter)
   

   Use

   Lentiviral

   Tags

   HA, eGFP, and p2A

   Expression

   Mammalian

   Mutation

   p.Arg95Ter mutation

   Promoter

   CMV

   Available since

   Dec. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. BE3-P2A-EGFP (pJUL977)

   Plasmid

   #123612

   Purpose

   CAG promoter expression plasmid for rAPOBEC1-XTEN-hSpCas9n(D10A)-UGI-NLS(SV40)-P2A-EGFP.

   Depositor

   Keith Joung

   Article

   Grunewald et al Nature. 2019 Apr 17. pii: 10.1038/s41586

   Insert

   BE3-P2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   D10A in SpCas9

   Promoter

   CAG

   Available since

   April 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMK377 (Hygro-P2A-mCherry2)

   Plasmid

   #121188

   Purpose

   N-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

   Insert

   Hygro-P2A-mCherry2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. peAAV-CKII(0.4)-Chronos_eGFP_GP2A_nls_mScarlet

   Plasmid

   #121542

   Purpose

   Chronos with red nucleus-targeted fluorescence for excitatory neurons

   Depositor

   Matthias Prigge

   Insert

   blue-absorbing channelrhodopsin Chronos with eGFP and nls-mScarlet
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mutation

   Promoter

   CamKII

   Available since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. katushka-FMDV-P2A-pTRIP

   Plasmid

   #163522

   Purpose

   Used to construct lentivirus to express katushka in mammalian cells with option for a second insert

   Depositor

   Thomas Weber

   Insert

   stopless Katushka-P2A
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRepair-MPP8_mAID-P2A-BFP

   Plasmid

   #175036

   Purpose

   Homology Directed Repair construct for C-terminal tagging of mMPP8 with mAID using CRISPR/Cas9

   Depositor

   Kristian Helin

   Article

   Muller et al Nat Commun. 2021 May 24;12(1):3034. doi:

   Insert

   mAID-P2A-BFP
   

   Use

   Hdr donor template

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-SpCas9-P2A-puro

    Plasmid

    #214358

    Purpose

    Expresses SpCas9 and puromycin resistance genes

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SpCas9 and puromycin resistance genes
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. MSCV-LMP2A-IRES-GFP

    Plasmid

    #220352

    Purpose

    Expresses EBV latent gene LMP2A in mammalian cells

    Depositor

    Klaus Rajewsky

    Article

    Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

    Insert

    LMP2A (LMP-2A Epstein-Barr Virus (EBV) strain B95-8)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-xCas9RA-P2A-Puro

    Plasmid

    #110871

    Purpose

    Lentiviral vector for constitutive expression of xCas9(3.7)RA in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    xCas9(3.7)RA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    A262T, R324L, S409I, E480K, E543D, M694I, E1219V

    Promoter

    EF1s

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJL2-mGold-P2A-EBFP2

    Plasmid

    #157998

    Purpose

    Co-expression of mGold and EBFP2 in mammalian cells.

    Depositor

    Francois St-Pierre

    Article

    Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

    Insert

    mGold-P2A-EBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mGold is mVenus with L46F;T63S mutations

    Promoter

    CMV

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMK451 (BSD-P2A-dTAG)

    Plasmid

    #214381

    Purpose

    dTAG for N-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    BSD-P2A-dTAG
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-postASAP-p2a-ChrimsonR

    Plasmid

    #178794

    Purpose

    Expresses voltage sensor targeted to dendrites and spines along with ChrimsonR

    Depositor

    Rafael Yuste

    Article

    Cornejo et al Science. 2022 Jan 7;375(6576):82-86. doi

    Insert

    FingR.PSD95, ASAP2f and ChrimsonR
    

    Use

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    mutations in amino acids L146G, S147T N149R, S150…

    Promoter

    CAG

    Available since

    Dec. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFH2.11_Gag-MCP_ZF2-P2A-eGFP

    Plasmid

    #205526

    Purpose

    Express HIV Gag-MCP_ZF2-P2A-eGFP

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    Gag-MCP_ZF2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMKO.1-Puro-shPP2A-Aalpha

    Plasmid

    #15249

    Depositor

    William Hahn

    Article

    Sablina et al Cell. 2007 Jun 1. 129(5):969-82.

    Insert

    PP2A regulatory subunit A, alpha isoform (PPP2R1A Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 12, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pLenti-ABE7.10-P2A-Puro

    Plasmid

    #112674

    Purpose

    Lentivirus for constitutive expression of ABE7.10 (not codon optimized)

    Depositor

    Lukas Dow

    Insert

    ABE7.10
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    D10A

    Promoter

    EF1s

    Available since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. SOX2-P2A-tagBFP-HDR

    Plasmid

    #163751

    Purpose

    HDR knock-in template for tagging the human endogenous SOX2 gene with a tagBFP fluorescent marker linked by a self-cleaving P2A peptide.

    Depositor

    Jennifer Mitchell

    Article

    Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

    Insert

    SOX2 (SOX2 Human)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    P2A-tagBFP

    Expression

    Mutation

    Does not contain start codon to avoid random inte…

    Promoter

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMK322 (TOP2A-mAC (Hygro))

    Plasmid

    #140653

    Purpose

    TOP2A tagging with mAID-Clover

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    TOP2A (TOP2A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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