We narrowed to 1,479 results for: pbluescript

1. pT2-eab2-[GTR]

   Plasmid

   #46144

   Depositor

   Wenbiao Chen

   Article

   Boniface et al Genesis. 2009 Jul;47(7):484-91. doi: 10.

   Insert

   EGFP/mCherry
   

   Use

   Cre/Lox; Tol2 zebrafish transgenic expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. foxn4

   Plasmid

   #11260

   Depositor

   Lisa Steiner

   Article

   Danilova et al Brain Res Dev Brain Res. 2004 Oct 15. 15

   Insert

   foxn4 (foxn4 Zebrafish)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

3. pMK376 (Hygro-P2A-mClover)

   Plasmid

   #121187

   Purpose

   N-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

   Insert

   Hygro-P2A-mClover
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGTag Hygro EGFP nLAP +0

   Plasmid

   #194305

   Purpose

   CRISPR/Cas9-mediated generic protein tagging

   Depositor

   Frank Schnütgen

   Article

   Thone et al Methods. 2019 Jul 15;164-165:59-66. doi:

   Insert

   Hygro-P2A-nLAP(EGFP)
   

   Use

   Bacterial cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJJS010

   Plasmid

   #188333

   Purpose

   PCR template for repair templates to tag a gene with a mKate2 and mIAA7 AID degron using homology-directed repair for C. elegans.

   Depositor

   Mike Boxem

   Article

   Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

   Insert

   Linker::mKate2::mIAA7
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJJS004

   Plasmid

   #188327

   Purpose

   PCR template for repair templates to tag a gene with a mCherry and mIAA7 AID degron using homology-directed repair for C. elegans.

   Depositor

   Mike Boxem

   Article

   Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

   Insert

   Linker::mCherry::mIAA7
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBS-SKII-3XHA-fSNAP-NAT

   Plasmid

   #188916

   Purpose

   Integration cassette to create C-terminal fusions for fluorescent tagging

   Depositor

   Stephen Buratowski

   Article

   Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

   Insert

   3XHA-fSNAP-NAT
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBS-SKII-3XHA-HALO-Phleomycin

   Plasmid

   #188930

   Purpose

   Integration cassette to create C-terminal fusions for fluorescent tagging

   Depositor

   Stephen Buratowski

   Article

   Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

   Insert

   3XHA-HALO-Phleomycin
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pC-PTP-NEO

   Plasmid

   #51710

   Purpose

   contains a C-terminal tandem affinity purification tag (relevant to any system) within a construct designed for genome integration into trypanosomes.

   Depositor

   Arthur Gunzl

   Article

   Schimanski et al Eukaryot Cell. 2005 Nov;4(11):1942-50.

   Type

   Empty backbone

   Use

   T. brucei genome integration vectors

   Tags

   PTP (ProtC-TEV-ProtA)

   Expression

   Mutation

   Promoter

   Available since

   March 12, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pKSB-611U6-sgRNA3 AGAG AACA

    Plasmid

    #173673

    Purpose

    To clone and express a gRNA in Anopheles

    Depositor

    Eric Marois

    Article

    Klug et al PLoS Pathog. 2022 Oct 12;18(10):e1010881

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBluescriptKS(-)-FRB

    Plasmid

    #104475

    Purpose

    Plasmid carrying FRB with C-terminal HA tag.

    Depositor

    Benjamin Glick

    Article

    Papanikou et al Elife. 2015 Dec 28;4. pii: e13232. doi:

    Insert

    FRB (MTOR Human)
    

    Use

    Phagemid

    Tags

    HA tag

    Expression

    Mutation

    includes only the FRB domain

    Promoter

    Available since

    Jan. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Halo-Erk2 donor

    Plasmid

    #113104

    Purpose

    Donor plasmid for knock-in HaloTag fusing to the N-terminal of mouse Erk2 coding sequence

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    N terminal Erk2 donor region including homology arms and HaloTag
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pPEE17

    Plasmid

    #128361

    Purpose

    Detecting gene essentiality in Cryptococcus neoformans. Targets DNA constructs to the Safe Haven Site 1, a small gene-free region. Contains amdS2 counterselectable marker. This version of the Safe Haven vector includes direct repeats, enabling the formation of popouts on fluoroacetamide.

    Depositor

    James Fraser

    Article

    Erpf et al Fungal Genet Biol. 2019 Jun 17;131:10324

    Insert

    Acetamidase (amdS)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    TEF1

    Available since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. 3185 pBS Bax cDNA

    Plasmid

    #8754

    Depositor

    Stanley Korsmeyer

    Article

    Knudson et al Science 1995 Oct 6;270(5233):96-9.

    Insert

    Bax (Bax Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

15. pBS-SKII-3XHA-fSNAP-Kan

    Plasmid

    #188918

    Purpose

    Integration cassette to create C-terminal fusions for fluorescent tagging

    Depositor

    Stephen Buratowski

    Article

    Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

    Insert

    3XHA-fSNAP-Kan
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMB67

    Plasmid

    #47947

    Purpose

    Expresses C. elegans optimized Cas9 from the hsp-16.48 promoter

    Depositor

    Mike Boxem

    Article

    Waaijers et al Genetics. 2013 Aug 26.

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and egl-13 NLS

    Expression

    Worm

    Mutation

    Promoter

    hsp-16.48

    Available since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMK409 (mAID-Nluc BSD)

    Plasmid

    #121199

    Purpose

    C-terminal tagging

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

    Insert

    mAID-Nluc BSD
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBlue-TAL

    Plasmid

    #49401

    Purpose

    The pBlue-TAL is suitable for TALEN mutagenesis in insects. It is compatible with Golden Gate Cloning Kit. It has Esp3I cleavage sites and a T7 promoter.

    Depositor

    Michal Zurovec

    Article

    Takasu et al PLoS One. 2013 Sep 18;8(9):e73458. doi:

    Type

    Empty backbone

    Use

    TALEN

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Dec. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBS Rax (CC#5)

    Plasmid

    #15138

    Depositor

    Connie Cepko

    Article

    Furukawa et al Proc Natl Acad Sci U S A. 1997 Apr 1. 94

    Insert

    Rax (Rax Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 15, 2007

    Availability

    Academic Institutions and Nonprofits only

20. B1979

    Plasmid

    #59181

    Purpose

    Expresses optimized blasticidin S resistance gene (BSD) for C. elegans

    Depositor

    Craig Mello

    Article

    Kim et al Genetics. 2014 May 30. pii: genetics.114

    Insert

    Blasticidin S resistance
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    rpl-28

    Available since

    Aug. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge