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Plasmid#101011PurposeConstruct for expression of a calcium sensitive luciferase reporter, GFP-aequorin, and hygromycin resistance gene by a bidirectional promoterDepositorTypeEmpty backboneUseAlgae, nannochloropsisTagsExpressionMutationPromoterRibiAvailable sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only
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pNOC-stacked-aequorin
Plasmid#101010PurposeConstruct for expression of aequorin, calcium sensitive luciferase reporter and hygromycin resistance gene by a bidirectional promoterDepositorTypeEmpty backboneUseAlgae, nannochloropsisTagsExpressionMutationPromoterRibiAvailable sinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHnCBS1D
Plasmid#52065Purposeexpresses nine genes from the H. neapolitanus carboxysome operon and CSOS1D; IPTG-inducibleDepositorInsertCbbL, CbbS, CSOS2, CSOS3, CSOS4A, CSOS4B, CSOS1C, CSOS1A, CSOS1B, CSOS1D
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pet11a-ABLE
Plasmid#158627PurposeAmp resistant IPTG inducible 6xHis-TEV-ABLE protein for E. coli expressionDepositorInsertApixaban-Binding Helical Bundle
UseTags6x His-tag and TEV cleavage tagExpressionBacterialMutationPromoterT7Available sinceMarch 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBz118_His_CsoS2
Plasmid#140841PurposeHalothiobacillus neapolitanus CsoS2 with N-terminal His-tag, pET-14b backboneDepositorInsertCsoS2
UseTagsHexahistidine tagExpressionBacterialMutationPromoterT7Available sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNOC-stacked-GeNL-Ca
Plasmid#101003PurposeConstruct for fusion expression of GeNL calcium reporter and neomycin/G418 resistance gene by a bidirectional promoterDepositorTypeEmpty backboneUseAlgae, nannochloropsis expressionTagsExpressionMutationPromoterAvailable sinceMay 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPB_mU6_enh-gRNA_Puro-T2A-BFP
Plasmid#235571PurposeEnhanced guide RNA expression & genomic integration. Target gRNA sequences are cloned in via the BstXI and BlpI sites.DepositorInsertsgRNA backbone
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLz37_CB_operon_CsoS2_N1-N4
Plasmid#140856PurposeHalothiobacillus neapolitanus carboxysome operon with full length CsoS2. For heterologous expression of carboxysomes in E. coli.DepositorInsertCbbL, CbbS, CsoS2 (full-length), CsoSCA, CsoS4A, CsoS4B, CsoS1C, CsoS1A, CsoS1B, CsoS1D
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBz15_Strep_Rubisco
Plasmid#140839Purposewild-type Halothiobacillus neapolitanus Form I Rubisco with StrepII tag (CbbL-StrepII, CbbS), pET-14b backboneDepositorInsertForm IA Rubisco; CbbL, CbbS
UseTagsStrep IIExpressionBacterialMutationPromoterT7Available sinceJune 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNOC-CRISPR-HA
Plasmid#99370PurposeExpresses Cas9-HA and sgRNA. Hygromycin resistance.DepositorTypeEmpty backboneUseAlgae, nannochloropsis expressionTagsCas9-HAExpressionMutationPromoterRibi promoterAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-λN-βLac
Plasmid#162039PurposeExpresion of the fusion protein of the λN domain of the λ phage and ampR gene (encoding the enzyme β-lactamase without the native signal sequence)DepositorInsertλN domain of the λ phage and a β-lactamase fusion protein
UseTags6xHis tagExpressionBacterialMutationPromoterT7Available sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPB_TRE3G_scFV-sfGFP-Prdm9CD
Plasmid#235577PurposeDox-inducible expression of the catalytic-domain (CD) of epigenetic effector Prdm9 fused with scFV to programme H3K4me3DepositorInsertPrdm9 (Prdm9 Mouse)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPB_TRE3G_scFV-sfGFP-Prdm9CD-CatMut
Plasmid#235586PurposeDox-inducible expression of control catalytic-mutant Prdm9 CD fused with scFVDepositorInsertPrdm9 (Prdm9 Mouse)
UseCRISPRTagsExpressionMutationG282APromoterAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPB_TRE3G_scFV-sfGFP-Setd2CD
Plasmid#235579PurposeDox-inducible expression of the catalytic-domain (CD) of epigenetic effector Setd2 fused with scFV to programme H3K36me3DepositorInsertSetd2 (Setd2 Mouse)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPB_TRE3G_scFV-sfGFP-Ring1bCD
Plasmid#235574PurposeDox-inducible expression of the catalytic-domain (CD) of epigenetic effector Ring1b fused with scFV to programme H2AK119ub.DepositorInsertRing1b (Rnf2 Mouse)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNOC-CRISPR-GFP
Plasmid#100009PurposeExpresses Cas9-GFP and sgRNA in Nannochloropsis oceanica, contains hygromycin resistance cassette, for integration into geneomeDepositorTypeEmpty backboneUseAlgae, nannochloropsis expressionTagsCas9-GFPExpressionMutationPromoterRibi promoterAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNOC-ARS-CRISPR-v2
Plasmid#99863PurposeExpresses Cas9-Nlux and sgRNA. Compact backbone contains CEN/ARS. Hygromycin selection marker.DepositorTypeEmpty backboneUseAlgae, nannochloropsisTagsExpressionMutationPromoterRibiAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
p1A
Plasmid#162692PurposeExpresses H. neapolitanus CbbLS and S. elongatus Prk. Rescues CCMB1 in M9 glycerol at 10% CO2DepositorInsertH. neapolitanus CbbLS and S. elongatus Prk
UseTagsN terminal 6x His tag on PRKExpressionBacterialMutationPromoterPLtet0-1 promoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNSA-ARS-CRISPR-BlastR
Plasmid#101009PurposeExpresses Cas9-Nlux and sgRNA and contains CEN/ARS. CCMP537 LDSP promoter driven Blasticidin resistance gene.DepositorTypeEmpty backboneUseAlgae, nannochloropsisTagsExpressionMutationPromoterAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-B4E
Plasmid#162067PurposeFor expresion of the B4E fusion protein, consists of the ampR gene (encoding the enzyme β-lactamase without the native signal sequence) fused to residues 28-215 of the murine eIF4EDepositorInsertampR-eIF4E fusion protein
UseTags6xHis tagExpressionBacterialMutationeIF4E incorporating the K119A mutation, which is …PromoterT7Available sinceFeb. 25, 2021AvailabilityAcademic Institutions and Nonprofits only