165,562 results

1. pcDNA3.1(+)-RIα-EGFP

   Plasmid

   #181840

   Purpose

   GFP-tagged PKA RIα subunit for mammalian expression.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   RIα-EGFP (PRKAR1A Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. VPX-mRNA

   Plasmid

   #227679

   Purpose

   plasmid for VPX mRNA IVT

   Depositor

   Scot Wolfe

   Article

   Liu et al Nat Biotechnol. 2024 Sep 25. doi: 10.103

   Insert

   VPX (HIV2gp3 )
   

   Expression

   Mammalian

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. p53-TRE-mStrawberry reporter

   Plasmid

   #158684

   Purpose

   This plasmid is a p53 pathway reporter. It has two copies of wild-type p53 binding sites promoter driving the expression of mStrawberry-pGK-BSD

   Depositor

   Ravid Straussman

   Article

   Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

   Insert

   mStrawberry
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. DYRK1A/pTRE3G-FL-hXIST

   Plasmid

   #149608

   Purpose

   Express doxycycline-inducible full length of human XIST cDNA

   Depositor

   Jeanne Lawrence

   Article

   Jiang et al Nature. 2013 Aug 15;500(7462):296-300. d

   Insert

   X-inactive specific transcript (XIST Human)
   

   Expression

   Mammalian

   Promoter

   pTRE3G

   Available Since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. Tn7 integration plasmid

   Plasmid

   #154134

   Purpose

   Suicidal integration plasmid with the Tn7 arms, streptomycin and kanamycin resistance genes and the restriction site to clone the barcode-carrying cassette.

   Depositor

   Shimon Bershtein

   Article

   Jasinska et al Nat Ecol Evol. 2020 Feb 24. pii: 10.1038

   Inserts

   left and right end of Tn7 arm
   Spectinomycin resistant gene
   

   Expression

   Bacterial

   Promoter

   Spectinomycin resistance gene promoter and no

   Available Since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCI-Caspase 1

   Plasmid

   #41552

   Depositor

   Kate Fitzgerald

   Article

   Hornung et al Nature. 2009 Mar 26. 458(7237):514-8.

   Insert

   Caspase-1 (CASP1 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV immediate-early enhancer/promoter

   Available Since

   Dec. 20, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. Active Wnt10B-V5

   Plasmid

   #43823

   Depositor

   Xi He

   Article

   MacDonald et al J Biol Chem. 2014 May 19. pii: jbc.M114.

   Insert

   WNT10B (WNT10B Human)
   

   Tags

   V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLEXSY-hyg2.1_LtIFT43_Flag

   Plasmid

   #194433

   Purpose

   Expression of a flag tagged version of L. tarentolae intraflagellar transport protein-43 (IFT43) in Leishmania tarentolae

   Depositor

   Alan Brown

   Article

   Meleppattu et al Cell. 2022 Dec 22;185(26):4986-4998.e12.

   Insert

   Intraflagellar transport complex subunit 43
   

   Use

   Leishmania tarentolae expression

   Tags

   3X Flag and Linker

   Promoter

   L.donovani promoter elements

   Available Since

   June 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR223-ERBB3

   Plasmid

   #23874

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   ERBB3 (ERBB3 Human)
   

   Use

   Gateway donor vector

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3 mHIF-1α MYC (P402A/P577A/N813A)

    Plasmid

    #44028

    Depositor

    Celeste Simon

    Article

    Hu et al Mol Biol Cell. 2007 Nov;18(11):4528-42.

    Insert

    mHIF-1α (P402A/P577A/N813A) (Hif1a Mouse)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    P402A, P577A, N813A

    Promoter

    CMV

    Available Since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. CMV-CASANOVA

    Plasmid

    #113035

    Purpose

    CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    CASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAS134

    Plasmid

    #211505

    Purpose

    AAV genome to express Venus under control of EF1a. Use for tri-color experiments to define injection conditions for AAV-Perturb-seq

    Depositor

    Randall Platt

    Article

    Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:

    Insert

    Venus
    

    Use

    AAV, CRISPR, Cre/Lox, and Mouse Targeting

    Expression

    Mammalian

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. VPS13A^Halo

    Plasmid

    #118759

    Purpose

    VPS13A internally tagged with Halo at residue 1372.

    Depositor

    Pietro De Camilli

    Article

    Kumar et al J Cell Biol. 2018 Oct 1;217(10):3625-363

    Insert

    VPS13A (VPS13A Human)
    

    Tags

    Halo tag inserted at position 1372

    Expression

    Mammalian

    Available Since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAVS1-Puro Xlone-eGFP

    Plasmid

    #136936

    Purpose

    donor plasmid for inducible transgene expression in human cells

    Depositor

    Xiaoping Bao

    Article

    Chang et al Biotechnol Bioeng. 2020 Apr 11. doi: 10.

    Insert

    all-in-one tet-on system
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    TRE3GS

    Available Since

    April 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. A1-20B1(P004)

    Plasmid

    #184056

    Purpose

    This plasmid carries Yn situ preamplifier A1-20B1 sequence and is used for nickase based synthesis.

    Depositor

    C. Ron Yu

    Article

    Wu et al Cell Rep Methods. 2022 Apr 18;2(4):10020

    Insert

    A1-20B1 preamplifier
    

    Use

    Synthetic Biology

    Available Since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBI-CMV1-mir144-CMV2-mir1

    Plasmid

    #164634

    Purpose

    Express miR-144 and miR-1-1 in mammalian cells

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Inserts

    mir144 (MIR144 Human)
    mir-1-1 (MIR1-1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pscAAV-Sox2-mStr

    Plasmid

    #135617

    Purpose

    Donor vector for genomic targeting of a P2A-mStrawberry cassette to the 3' end of the mouse Sox2 locus

    Depositor

    Lin He

    Article

    Chen et al Cell Rep. 2019 Jun 25;27(13):3780-3789.e

    Insert

    P2A-mStrawberry flanked by Sox2 homology arms (Sox2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    P2A-mStrawberry

    Available Since

    May 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR-FUSN-mCh-Cry2olig

    Plasmid

    #101224

    Purpose

    FUS(1-214) fused to mCh-Cry2olig

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

    Insert

    FUS(aa1-214) (FUS Human)
    

    Use

    Lentiviral

    Tags

    mCherry-Cry2olig

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Feb. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCR2.1-InC

    Plasmid

    #83959

    Purpose

    Contains a synthetic insert to serve as internal control DNA template for a Salmonella Real-Time PCR assay.

    Depositor

    Kai-Shun Chen

    Insert

    Internal Control sequence
    

    Expression

    Bacterial

    Available Since

    Nov. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-Flag-IDH1

    Plasmid

    #62906

    Purpose

    Expresses wildtype IDH1 in mammalian cells

    Depositor

    Yue Xiong

    Article

    Zhao et al Science. 2009 Apr 10;324(5924):261-5. do

    Insert

    isocitrate dehydrogenase 1 (NADP+), soluble (IDH1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    April 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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