We narrowed to 11,282 results for: Vars;

1. pJOG639

   Plasmid

   #105390

   Purpose

   Synthetic biology

   Depositor

   Johannes Stuttmann

   Article

   Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

   Insert

   promotor CaBs3 (1000bp), variant
   

   Use

   Synthetic Biology

   Mutation

   TALE-binding site; BsaI/ BpiI restriction sites r…

   Available Since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJOG142

   Plasmid

   #105397

   Purpose

   Synthetic biology

   Depositor

   Johannes Stuttmann

   Article

   Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

   Insert

   mYFP (GFP variant) fused to PKI nuclear export signal
   

   Use

   Synthetic Biology

   Mutation

   BsaI/ BpiI restriction sites removed

   Available Since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJOG143

   Plasmid

   #105398

   Purpose

   Synthetic biology

   Depositor

   Johannes Stuttmann

   Article

   Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

   Insert

   mYFP (GFP variant) fused to SV40 nuclear localization signal
   

   Use

   Synthetic Biology

   Mutation

   BsaI/ BpiI restriction sites removed

   Available Since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. P0050::kaiBC S431A T432A NS1

   Plasmid

   #101837

   Purpose

   drives expression of kaiBC S431A T432A variant from an rpaA-independent promoter in NS1 locus

   Depositor

   Erin O'Shea

   Article

   Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

   Insert

   kaiBC S431A T432A with p0050 promoter
   

   Use

   Cyanobacteria cloning vector

   Mutation

   S431A T432A

   Available Since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. P0050::kaiBC S431E T432E NS1

   Plasmid

   #101836

   Purpose

   drives expression of kaiBC S431E T432E variant from an rpaA-independent promoter in NS1 locus

   Depositor

   Erin O'Shea

   Article

   Puszynska et al Elife. 2017 Apr 21;6. pii: e23210. doi:

   Insert

   kaiBC S431E T432E with p0050 promoter
   

   Use

   Cyanobacteria cloning vector

   Mutation

   S431E T432E

   Available Since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pACYC_HA-ZmSAE1_ZmSAE2(TRUNC)-FLAG

   Plasmid

   #91945

   Purpose

   Bacterial expression of maize SAE1 and truncated SAE2

   Depositor

   Richard Vierstra

   Article

   Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

   Inserts

   HA-ZmSAE1
   ZmSAE2-FLAG
   

   Tags

   FLAG and HA

   Expression

   Bacterial

   Mutation

   Truncation of ZmSAE2 to mimic a splice variant

   Available Since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. p3R112C/T180C-GG

   Plasmid

   #85093

   Purpose

   Host vector to fuse a gene at the C-terminus of PCNA3 variant (R112C/T180C)

   Depositor

   Teruyuki Nagamune

   Article

   Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

   Insert

   DNA polymerase sliding clamp 3
   

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   changed Arginine 112 to Cysteine and Threonine 18…

   Promoter

   T7 promoter

   Available Since

   Nov. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. p1G108C-GG

   Plasmid

   #85092

   Purpose

   Host vector to fuse a gene at the C-terminus of PCNA1 variant (G108C)

   Depositor

   Teruyuki Nagamune

   Article

   Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

   Insert

   DNA polymerase sliding clamp 1
   

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   changed Glycine 108 to Cysteine

   Promoter

   T7 promoter

   Available Since

   Nov. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. MSCV-6NBC-GFP-PGK-Puro-Barcode9

   Plasmid

   #83686

   Purpose

   individual 6N barcode to make barcoded cell line variants

   Depositor

   Monte Winslow

   Article

   Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

   Insert

   Barcode9
   

   Use

   Retroviral

   Mutation

   please view depositor comments below

   Available Since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. MSCV-6NBC-GFP-PGK-Puro-Barcode7

    Plasmid

    #83684

    Purpose

    individual 6N barcode to make barcoded cell line variants

    Depositor

    Monte Winslow

    Article

    Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

    Insert

    Barcode7
    

    Use

    Retroviral

    Mutation

    L119P in GFP

    Available Since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX-KG/Net1A

    Plasmid

    #28261

    Depositor

    Jeffrey Frost

    Article

    Carr et al J Biol Chem. 2009 Sep 4. 284(36):24269-8

    Insert

    Net1A (Net1 Mouse)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    April 11, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pSB700 Cerulean-Weissman scaffold

    Plasmid

    #79377

    Purpose

    Cas9 gRNA variant to recruit ms2 proteins

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    scaffold
    

    Use

    CRISPR

    Expression

    Mammalian

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSB700 Cerulean-Zhang2.0

    Plasmid

    #79378

    Purpose

    Cas9 gRNA variant to recruit ms2 proteins

    Depositor

    George Church

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    zhang 2.0
    

    Use

    CRISPR

    Expression

    Mammalian

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJRH-1362 scFv entry plasmid

    Plasmid

    #201912

    Purpose

    Entry vector for cloning single chain variable fragments displayed on the human CD8a hinge and transmembrane domain (CAG promoter)

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Insert

    Stuffer sequence (drop out for scFv cloning) + CD8 hinge and transmembrane domain
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. tau/pET29b

    Plasmid

    #16316

    Depositor

    Peter Klein

    Article

    Hedgepeth et al Dev Biol. 1997 May 1. 185(1):82-91.

    Insert

    tau (MAPT Human)
    

    Expression

    Bacterial

    Available Since

    Dec. 21, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAF0451 Si74_attP-mCherry donor

    Plasmid

    #193470

    Purpose

    Promoterless mcherry donor for Si74

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Si74_attP
    

    Expression

    Mammalian

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAF0474 Ef1a-Si74_attB acceptor

    Plasmid

    #193475

    Purpose

    Acceptor plasmid for 3 plasmid recombination assay

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Si74_attB
    

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGP-CAG-iGABASnFR2-WPRE-bGH-polyA

    Plasmid

    #218868

    Purpose

    Mammalian expression of improved GABA sensor (positive change in fluorescence)

    Depositor

    GENIE Project

    Article

    Kolb et al bioRxiv 2025.03.25.644953

    Insert

    iGABASnFR2
    

    Expression

    Mammalian

    Mutation

    S99A F102Y F104Y L178S

    Promoter

    CAG

    Available Since

    May 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAF0443 Nm60_attP-mCherry donor

    Plasmid

    #193471

    Purpose

    Promoterless mcherry donor for Nm60

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Nm60_attP
    

    Expression

    Mammalian

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAF0466 Ef1a-Nm60_attB acceptor

    Plasmid

    #193476

    Purpose

    Acceptor plasmid for 3 plasmid recombination assay

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Nm60_attB
    

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pAF0185 Cp36_attP-mCherry donor

    Plasmid

    #193468

    Purpose

    Promoterless mcherry donor for Cp36

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Cp36_attP
    

    Expression

    Mammalian

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. pAF0286 Ef1a-Cp36_attB-BFP acceptor

    Plasmid

    #193474

    Purpose

    Acceptor plasmid for 3 plasmid recombination assay

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Cp36_attB
    

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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23. pCK007 pME KalTa4

    Plasmid

    #195965

    Purpose

    Gateway pME Gal4 variant

    Depositor

    Alexa Burger, Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    KalTA4 (Gal4)
    

    Use

    Other

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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24. pCDNA3 Flag Jnk2a1

    Plasmid

    #13754

    Depositor

    Roger Davis

    Article

    Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

    Insert

    Jnk2a1 (MAPK9 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

25. pCDNA3 Flag Jnk1a2

    Plasmid

    #13751

    Depositor

    Roger Davis

    Article

    Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

    Insert

    Jnk1a2 (MAPK8 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

26. pNB23

    Plasmid

    #103058

    Purpose

    medium copy plasmid (pBR322 backbone) expressing the unstable GFP variant GFP-OVA under control of the hydrogen peroxide-inducible promoter katGp

    Depositor

    Dirk Bumann

    Article

    Burton et al Cell Host Microbe. 2014 Jan 15;15(1):72-

    Insert

    GFP-OVA
    

    Expression

    Bacterial

    Promoter

    hydrogen peroxide-inducible promoter katGp

    Available Since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pUAS-V5-miniTurboID-NES

    Plasmid

    #116905

    Purpose

    Expresses miniTurboID under Gal4/UAS control. miniTurboID is tagged at the N-terminus with V5 and a nuclear export signal (NES) at the C-terminus.

    Depositor

    Norbert Perrimon

    Article

    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

    Insert

    miniTurboID
    

    Use

    Drosophila expression

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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28. pAF0687 Pa01_attP-mCherry donor

    Plasmid

    #193466

    Purpose

    Promoterless mcherry donor for Pa01

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Pa01_attP
    

    Expression

    Mammalian

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGP-AAV-syn-iGABASnFR2-WPRE

    Plasmid

    #218874

    Purpose

    AAV-mediated expression of improved GABA sensor (positive change in fluorescence)

    Depositor

    GENIE Project

    Article

    Kolb et al bioRxiv 2025.03.25.644953

    Has Service

    AAV1 and AAV5

    Insert

    iGABASnFR2
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    S99A F102Y F104Y L178S

    Promoter

    Synapsin

    Available Since

    June 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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30. pAF0689 Ef1a-Pa01_attB-BFP acceptor

    Plasmid

    #193472

    Purpose

    Acceptor plasmid for 3 plasmid recombination assay

    Depositor

    Patrick Hsu

    Article

    Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

    Insert

    Pa01_attB
    

    Expression

    Mammalian

    Promoter

    Ef1a

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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