We narrowed to 58,550 results for: tra

1. pSL3617 (pCRISPR(BbsI)_Pse)

   Plasmid

   #200902

   Purpose

   Expresses Pseudoalteromonas CRISPR RNA from a U6 promoter. The CRISPR array contains two BbsI sites for spacer cloning.

   Depositor

   Samuel H. Sternberg

   Article

   Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

   Insert

   PseCRISPR (entry)
   

   Expression

   Mammalian

   Available Since

   May 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-C1-Ataxin3Q28

   Plasmid

   #22122

   Depositor

   Henry Paulson

   Article

   Chai et al Proc Natl Acad Sci U S A. 2002 Jul 9. 99

   Insert

   Ataxin 3 (ATXN3 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   Oct. 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNUT N6His hTFNG

   Plasmid

   #67240

   Purpose

   Expresses N-His tagged nonglycosylated human serum transferrin in mammalian cells

   Depositor

   Ross MacGillivray, Anne Mason

   Article

   Mason et al Biochemistry. 2002 Jul 30;41(30):9448-54

   Insert

   human serum transferrin (TF Human)
   

   Tags

   N-terminal signal peptide, 4 aa link, 6 His, Fact…

   Expression

   Mammalian

   Mutation

   Asn413 Asp, Asn611Asp

   Promoter

   SV40

   Available Since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYES260-sc.eEF2 K509A

   Plasmid

   #85120

   Purpose

   yeast expression of mutant EEF2

   Depositor

   Pal Falnes

   Article

   Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

   Insert

   EFT1/EFT2 (EFT2, EFT1 Budding Yeast)
   

   Tags

   6xHis

   Expression

   Yeast

   Mutation

   K509A

   Promoter

   GAL1

   Available Since

   Nov. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHT-6xR.ads-PxylA-DI

   Plasmid

   #47386

   Purpose

   Expresses ads under Pgrac promoter (inducible by IPTG) and expresses dxs and idi under PxylA promoter (inducible by xylose) in bacteria

   Depositor

   Heng-Phon Too

   Article

   Zhou et al Biotechnol Bioeng. 2013 Mar 8. doi: 10.1

   Inserts

   ads
   dxs
   idi
   

   Tags

   6xR and His

   Expression

   Bacterial

   Promoter

   Pgrac and PxylA

   Available Since

   Oct. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. retro-gRNA-eGFP

   Plasmid

   #116926

   Purpose

   Retrovirus expressing eGFP with BBSI cloning sites for sgRNA

   Depositor

   Christophe Benoist, Diane Mathis

   Article

   Magnuson et al Proc Natl Acad Sci U S A. 2018 Nov 6;115

   Insert

   eGFP
   

   Use

   Retroviral

   Promoter

   EF1a

   Available Since

   Oct. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDC-UAS-nBitbow1.0(miniW)

   Plasmid

   #138329

   Purpose

   Drosophila attB-targeting plasmid that contains UAS driving nBitbow1.0 cassette. Expresses combinations of nuclear mAmetrine, mTFP, mNeonGree, mKusabira-Orange2, and mKate2 upon Flp recombination.

   Depositor

   Dawen Cai

   Article

   Veling et al Cell Rep. 2019 Dec 3;29(10):3303-3312.e3

   Inserts

   mAmetrine
   tdKatushka2
   mNeonGreen
   mTFP1
   mKusabira-Orange2
   

   Tags

   human H2B

   Expression

   Insect

   Promoter

   10xUAS

   Available Since

   March 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV-hSyn-Soma-jGCaMP8s

   Plasmid

   #169256

   Purpose

   AAV transfer plasmid for neuronal expression of soma-targeted jGCaMP8s.

   Depositor

   Marianne Fyhn

   Article

   Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

   Has Service

   AAV Retrograde, AAV1, and AAV9

   Insert

   Soma-jGCaMP8s
   

   Use

   AAV

   Tags

   6xHis-tag

   Expression

   Mammalian

   Available Since

   May 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDonor-GDH

   Plasmid

   #140629

   Purpose

   Encodes a mini-transposon derived from V. cholerae Tn6677 and T7-GDH cargo gene.

   Depositor

   Sheng Yang

   Article

   Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

   Insert

   Mini-Tn6677, T7lac-GDH gene cassette
   

   Use

   CRISPR; Transposon

   Expression

   Bacterial

   Available Since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJL NarL YdfI Flag

    Plasmid

    #225650

    Purpose

    Expresses NarL with a mutated DNA binding domain (YdfI). For use with NarX chimeras and reporter plasmids from this study.

    Depositor

    Neha Kamat

    Article

    Peruzzi et al Proc Natl Acad Sci U S A. 2023 May 9;120

    Insert

    NarL-YdfI (narL Synthetic)
    

    Use

    Cell free expression

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    NarL with native DNA-binding domain replaced with…

    Promoter

    T7

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. GFP-rab7 DN

    Plasmid

    #12660

    Depositor

    Richard Pagano

    Article

    Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

    Insert

    RAB7 (Homo sapiens) (RAB7A Human)
    

    Tags

    GFP and HA

    Expression

    Mammalian

    Mutation

    T22N

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-pMecp2-dSaCas9-VP64-pU6-sgRNA

    Plasmid

    #158972

    Purpose

    Vector C encodes pAAV-pMecp2-dSaCas9-VP64-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in neurons

    Depositor

    Chung Tin

    Article

    Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

    Insert

    dSaCas9-VP64
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    pMecp2

    Available Since

    Sept. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. gRNA NIA TLS1

    Plasmid

    #196980

    Purpose

    Expresses two gRNAs targeting Arabidopsis NIA1 gene for knockout. Each gRNA is fused to TLS1 mobility signal that confers graft mobility.

    Depositor

    Friedrich Kragler

    Article

    Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    AtNIA1 gRNAs fused to TLS1 tags
    

    Expression

    Plant

    Promoter

    pU6-26, pU6-29

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMax_mOrange_G299C

    Plasmid

    #177829

    Purpose

    Site-specific mutagenesis of mOrange for the purposes of creating a site-specific G:G mispair for the purposes of measuring MMR activity via Host Cell Reactivation (HCR).

    Depositor

    Zachary Nagel, Leona Samson

    Article

    Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

    Insert

    mOrange_G299C
    

    Expression

    Mammalian

    Mutation

    G299C

    Promoter

    CMV

    Available Since

    Nov. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG/ATP10B-HA

    Plasmid

    #209216

    Purpose

    Mammalian expression of ATP10B

    Depositor

    Hye-Won Shin

    Article

    Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

    Insert

    ATP10B (ATP10B Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. p6352 MSCV-CMV-CMV-Flag-HA-JMJD6

    Plasmid

    #31358

    Depositor

    Peter Howley

    Article

    Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

    Insert

    JMJD6 (JMJD6 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Available Since

    Oct. 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pInducer20 EGFP-TEADi

    Plasmid

    #140145

    Purpose

    Tetracycline-inducible TEADi (GFP-tagged inhibitor of the interaction of YAP1 and TAZ with TEAD transcription factors with nuclear localization signal)

    Depositor

    Ramiro Iglesias-Bartolome

    Article

    Yuan et al Nat Commun. 2020 Mar 19;11(1):1472. doi:

    Insert

    EGFP TEADi
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    March 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. tetO-TCF21

    Plasmid

    #170706

    Purpose

    doxycycline-inducible overexpression of TCF21

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    TCF21 (TCF21 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Expression

    Mammalian

    Promoter

    TRE promoter, Tet-ON

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-pMecp2-dSaCas9-KRAB-pU6-sgRNA

    Plasmid

    #158988

    Purpose

    Vector E encodes pAAV-pMecp2-dSaCas9-KRAB-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR interference in neurons

    Depositor

    Chung Tin

    Article

    Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

    Insert

    dSaCas9-KRAB
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Promoter

    pMecp2

    Available Since

    Aug. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. scFLARE1-p2A-eGFP

    Plasmid

    #158699

    Purpose

    Mammalian expression of scFLARE1 component bearing a p2A-eGFP as transfection marker

    Depositor

    Alice Ting

    Article

    Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

    Insert

    scFLARE1-p2A-eGFP
    

    Use

    AAV

    Tags

    Flag, V5. eGFP

    Expression

    Mammalian

    Mutation

    Truncated form of TEV protease (220-242 amino aci…

    Promoter

    CMV

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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